Human/Mouse TGF-beta RI/ALK-5 Antibody Summary
Ala21-Glu121
Accession # BAA05023
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Mouse TGF-beta RI/ALK-5 by Western Blot TGFRI and TGFRII association in fibulin-6 KD cells upon TGF-beta stimulus.scr siRNA or fibulin-6 siRNA transfected nCF, after TGF-beta stimulation are subjected to (a) FACS analysis. No difference in the amount of TGF beta RI and TGF beta RII on the surface of control and fibulin-6 KD cells was observed. (b) Immuno-precipitation of TGFRII from cell lysates of scr transfected and TGF-beta stimulated cells, shows more accumulation of TGFRI after western blotting compared to non TGF-beta treated nCF. (c) Immuno-precipitation of TGFRII followed by WB for TGFRI from cell lysates of scr transfected or fibulin-6 KD cells after TGF-beta stimulation display reduced association of receptors in fibulin-6 KD condition. The control input lanes shows no difference and IgG control is also clean (n = 4, p < 0.05). (d) Phosphorylation status of TGFRI was analyzed by western blot using phospho-TGFRI specific antibody. Densitometric analysis of western blot display decreased phosphorylation of TGFRI in fibulin-6 KD cells after TGF-beta stimulation compared to control cells (n = 5, p < 0.01, nonparametric Mann-Whitney U test). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28209981), licensed under a CC-BY license. Not internally tested by R&D Systems.
View Larger
Detection of Mouse TGF-beta RI/ALK-5 by Western Blot TGFRI and TGFRII association in fibulin-6 KD cells upon TGF-beta stimulus.scr siRNA or fibulin-6 siRNA transfected nCF, after TGF-beta stimulation are subjected to (a) FACS analysis. No difference in the amount of TGF beta RI and TGF beta RII on the surface of control and fibulin-6 KD cells was observed. (b) Immuno-precipitation of TGFRII from cell lysates of scr transfected and TGF-beta stimulated cells, shows more accumulation of TGFRI after western blotting compared to non TGF-beta treated nCF. (c) Immuno-precipitation of TGFRII followed by WB for TGFRI from cell lysates of scr transfected or fibulin-6 KD cells after TGF-beta stimulation display reduced association of receptors in fibulin-6 KD condition. The control input lanes shows no difference and IgG control is also clean (n = 4, p < 0.05). (d) Phosphorylation status of TGFRI was analyzed by western blot using phospho-TGFRI specific antibody. Densitometric analysis of western blot display decreased phosphorylation of TGFRI in fibulin-6 KD cells after TGF-beta stimulation compared to control cells (n = 5, p < 0.01, nonparametric Mann-Whitney U test). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28209981), licensed under a CC-BY license. Not internally tested by R&D Systems.
View Larger
Detection of Mouse TGF-beta RI/ALK-5 by Flow Cytometry TGFRI and TGFRII association in fibulin-6 KD cells upon TGF-beta stimulus.scr siRNA or fibulin-6 siRNA transfected nCF, after TGF-beta stimulation are subjected to (a) FACS analysis. No difference in the amount of TGF beta RI and TGF beta RII on the surface of control and fibulin-6 KD cells was observed. (b) Immuno-precipitation of TGFRII from cell lysates of scr transfected and TGF-beta stimulated cells, shows more accumulation of TGFRI after western blotting compared to non TGF-beta treated nCF. (c) Immuno-precipitation of TGFRII followed by WB for TGFRI from cell lysates of scr transfected or fibulin-6 KD cells after TGF-beta stimulation display reduced association of receptors in fibulin-6 KD condition. The control input lanes shows no difference and IgG control is also clean (n = 4, p < 0.05). (d) Phosphorylation status of TGFRI was analyzed by western blot using phospho-TGFRI specific antibody. Densitometric analysis of western blot display decreased phosphorylation of TGFRI in fibulin-6 KD cells after TGF-beta stimulation compared to control cells (n = 5, p < 0.01, nonparametric Mann-Whitney U test). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28209981), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta RI/ALK-5
Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I receptor, originally known as ALK-5 (Activin receptor‑like kinase) is also a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors.
- Miyazono, K. et al. (1994) Adv. In Immunol. 55:181.
- Massagùe, J. (1998) Ann. Rev. Biochem. 67:753.
Product Datasheets
Citations for Human/Mouse TGF-beta RI/ALK-5 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
11
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Cholesterol Pathway Inhibition Induces TGF-beta Signaling to Promote Basal Differentiation in Pancreatic Cancer
Authors: Gabitova-Cornell L, Surumbayeva A, Peri S et al.
Cancer Cell
-
Cationic proteins from eosinophils bind bone morphogenetic protein receptors promoting vascular calcification and atherogenesis
Authors: Meng Z, Zhang S, Li W et al.
European heart journal
-
Regulating the cell shift of endothelial cell-like myofibroblasts in pulmonary fibrosis
Authors: Wu X, Zhang D, Qiao X et al.
The European respiratory journal
-
TGF-?1 induces PD-1 expression in macrophages through SMAD3-STAT3 cooperative signaling in chronic inflammation
Authors: Lei, Z;Tang, R;Wu, Y;Mao, C;Xue, W;Shen, J;Yu, J;Wang, X;Qi, X;Wei, C;Xu, L;Zhu, J;Li, Y;Zhang, X;Ye, C;Chen, X;Yang, X;Zhou, S;Su, C;
JCI insight
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Role of Nox4 in mitigating inflammation and fibrosis in dextran sulfate sodium-induced colitis
Authors: Lee, Y;Kim, SH;Jeong, H;Kim, KH;Jeon, D;Cho, Y;Lee, D;Nam, KT;
Cellular and molecular gastroenterology and hepatology
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Prevention of bleomycin-induced pulmonary fibrosis by a RANKL peptide in mice
Authors: N Ju, H Hayashi, M Shimamura, S Baba, S Yoshida, R Morishita, H Rakugi, H Nakagami
Scientific Reports, 2022-07-21;12(1):12474.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Fibulin-6 regulates pro-fibrotic TGF-beta responses in neonatal mouse ventricular cardiac fibroblasts.
Authors: Chowdhury A, Hasselbach L, Echtermeyer F, Jyotsana N, Theilmeier G, Herzog C
Sci Rep, 2017-02-17;7(0):42725.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, Western Blot -
Differential TGFbeta pathway targeting by miR-122 in humans and mice affects liver cancer metastasis.
Authors: Yin S, Fan Y, Zhang H, Zhao Z, Hao Y, Li J, Sun C, Yang J, Yang Z, Yang X, Lu J, Xi J
Nat Commun, 2016-03-18;7(0):11012.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Beclin 1 regulates neuronal transforming growth factor-beta signaling by mediating recycling of the type I receptor ALK5.
Authors: O'Brien C, Bonanno L, Zhang H, Wyss-Coray T
Mol Neurodegener, 2015-12-21;10(1):69.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC-Fr -
Dendritic cell SIRT1-HIF1alpha axis programs the differentiation of CD4+ T cells through IL-12 and TGF-beta1.
Authors: Liu G, Bi Y, Xue L, Zhang Y, Yang H, Chen X, Lu Y, Zhang Z, Liu H, Wang X, Wang R, Chu Y, Yang R
Proc Natl Acad Sci U S A, 2015-02-17;112(9):E957-65.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Integrin alpha3beta1 potentiates TGFbeta-mediated induction of MMP-9 in immortalized keratinocytes.
Authors: Lamar JM, Iyer V, DiPersio CM
J. Invest. Dermatol., 2007-08-30;128(3):575-86.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human/Mouse TGF-beta RI/ALK-5 Antibody
Average Rating: 3.7 (Based on 3 Reviews)
Have you used Human/Mouse TGF-beta RI/ALK-5 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
R&D Systems Technical Service is investigating.
