Human/Mouse SWAP70 Antibody

Catalog #: MAB6735 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6735
MAB6735-SP

Detection of Human and Mouse SWAP70 by Western Blot.

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Human/Mouse SWAP70 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human SWAP70 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 683317

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human SWAP70
Arg386-Glu585
Accession # Q9UH65

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse SWAP70 by Western Blot. Western blot shows lysates of BJAB human Burkitt's lymphoma cell line and L1.2 mouse pro-B cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse SWAP70 Monoclonal Antibody (Catalog # MAB6735) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SWAP70 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SWAP70

SWAP70 (Switch-associated protein 70) is a 67-70 kDa intracellular protein that bears limited resemblance to members of the Dbl-family of GEFs for small Rho GTPases. It is expressed in mast cells, dendritic cells and B cells. In mast cells, it promotes the release of anaphylactic mediators; in dendritic cells, it regulates the appearance of MHC II; and in B cells, it forms a nuclear complex that initiates immunoglobulin class switching. Human SWAP70 is 585 amino acids (aa) in length. It contains one plextrin homology (PH) domain (aa 213-304) plus a coiled-coil region (aa 316-539). There are three potential splice variants. One contains an alternative start site at Met70, another shows a deletion of aa 81-138, and a third possesses a 38 aa substitution for aa 453-585. Over aa 386-585, human SWAP70 shares 96% aa identity with mouse SWAP70.

Long Name

Switch-associated Protein 70 kDa

Entrez Gene IDs

23075 (Human); 20947 (Mouse); 293410 (Rat)

Alternate Names

KIAA0640SWAP-70FLJ39540; SWAP switching B-cell complex 70kDa subunit; SWAP70; switch-associated protein 70

Product Datasheets

Product Datasheet

COA

SDS

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