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Human/Mouse Sulfatase Modifying Factor 1/SUMF1 Antibody

Catalog #: MAB2779 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2779
MAB2779-SP
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Human/Mouse Sulfatase Modifying Factor 1/SUMF1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects recombinant human or mouse Sulfatase Modifying Factor 1/SUMF1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human SUMF2 or recombinant mouse SUMF2 is observed.
Source
Monoclonal Mouse IgG2B Clone # 329005
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Sulfatase Modifying Factor 1/SUMF1 isoform 1
Ser34-Asp374 (Ser63Asn)
Accession # Q8NBK3.3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human and mouse Sulfatase Modifying Factor 1/SUMF1
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human or Recombinant Mouse Sulfatase Modifying Factor 1/SUMF1, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sulfatase Modifying Factor 1/SUMF1

Also known as C-alpha -formylglycine-generating enzyme (FGE), SUMF1 converts the active site Cys residue of sulfatases into formylglycine, which is essential for sulfatase activity. SUMF1 defect causes multiple sulfatase deficiency (MSD). The amino acid sequence of rhSUMF1 is 93%, 92% and 90% identical to that of canine, bovine, and mouse/rat.

Entrez Gene IDs
285362 (Human); 58911 (Mouse); 362409 (Rat)
Alternate Names
AAPA3037; EC 1.8.99.-; FGE1; FGEC-alpha-formylglycine-generating enzyme 1; FGly-generating enzyme; MGC131853; MGC150436; sulfatase modifying factor 1; sulfatase-modifying factor 1; SUMF1; UNQ3037

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