Home / STAT5a/b / Human/Mouse STAT5a/b Pan Specific Antibody

Human/Mouse STAT5a/b Pan Specific Antibody

Catalog #: AF2168
28 Citations 1 Review Datasheet / COA / SDS
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AF2168
AF2168-SP
Detection of Human/Mouse STAT5a/b by Western Blot.
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Citations (28)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse STAT5a/b Pan Specific Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects endogenous human and mouse STAT5A and STAT5B in Western blots.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Human STAT5a and STAT5b synthetic peptide
aa 219-390
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
20 µg/mL
See below
Chromatin Immunoprecipitation (ChIP)
5 µg/5 x 106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse STAT5a/b antibody by Western Blot. View Larger

Detection of Human/Mouse STAT5a/b by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and M1 mouse myeloid leukemia cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse STAT5a/b Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2168) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for STAT5a at approximately 94 kDa and STAT5b at approximately 92 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Chromatin Immunoprecipitation (ChIP) Detection of STAT5a/b-regulated Genes antibody by Chromatin Immunoprecipitation. View Larger

Detection of STAT5a/b-regulated Genes by Chromatin Immunoprecipitation. BaF3 mouse pro-B cell line treated with 10 ng/mL Recombinant Mouse IL-3 (Catalog # 403-ML) for 1 hour was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. STAT5a/b/DNA complexes were immunoprecipitated using 5 µg Rabbit Anti-Human/Mouse STAT5a/b Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2168) or control antibody (Catalog # AB-105-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Rabbit IgG Secondary Antibody (Catalog # BAF008). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Thebcl-xpromoter was detected by standard PCR.

Simple Western Detection of Human STAT5a/b antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human STAT5a/b by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for STAT5a/b at approximately 91 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human/Mouse STAT5a/b Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2168). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western Blot Detection of Human STAT5a/b by Western Blot View Larger

Detection of Human STAT5a/b by Western Blot Effects of hypoxia generated by using the insert-based enzymatic system on the phosphorylation of cell signalling molecules. Several molecules associated with cellular signalling and survival events are differentially phosphorylated between the hypoxia (H) and normoxia (N) groups at 2 hours after the hypoxic insult. One representative western blotting experiment is shown above the columns. Bars denote mean ± s.e.m. of three experiments; *P<0.05. Image collected and cropped by CiteAb from the following publication (https://journals.biologists.com/dmm/article/doi/10.1242/dmm.013078/258749/An-insert-based-enzymatic-cell-culture-system-to), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAT5a/b

Signal transduction and activator of transcription 5 (STAT5) is a member of the Jak/STAT signal transduction pathway and is activated by a variety of cytokines (IL22, IL6, IFN-α). STAT5 has two isoforms (A and B) that share 93% amino acid identity and bind the DNA consensus site TTCN3GAA. STAT5 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, translocates to the nucleus and activates transcription of specific genes. STAT5 is involved in a wide array of biological processes ranging from regulating apoptosis to adult mammary gland proliferation, differentiation and survival.

Long Name
Signal Transducer and Activator of Transcription 5b
Alternate Names
MGF; STAT5; STAT5A signal transducer and activator of transcription 5A ; STAT5a/b

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Citations for Human/Mouse STAT5a/b Pan Specific Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. An insert-based enzymatic cell culture system to rapidly and reversibly induce hypoxia: investigations of hypoxia-induced cell damage, protein expression and phosphorylation in neuronal IMR-32 cells.
    Authors: Huang Y, Zitta K, Bein B et al.
    Dis Model Mech.
  2. Evaluation of Roasting and Grilling Effects on Chemical Composition, Volatile Profiles, and Toxicity of Stink Bugs (Tessaratoma papillosa): Implications for Utilization as Functional Food Ingredients
    Authors: Li H, Chumroenphat T, Boonarsa P et al.
    Foods (Basel, Switzerland)
  3. Structure and function of a dual antagonist of the human growth hormone and prolactin receptors with site-specific PEG conjugates
    Authors: Basu, R;Brody, R;Sandbhor, U;Kulkarni, P;Davis, E;Swegan, D;Caggiano, LJ;Brenya, E;Neggers, S;Kopchick, JJ;
    The Journal of biological chemistry
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Batf stabilizes Th17 cell development via impaired Stat5 recruitment of Ets1‐Runx1 complexes
    Authors: Duy Pham, Daniel J Silberger, Kim N Nguyen, Min Gao, Casey T Weaver, Robin D Hatton
    The EMBO Journal
  5. Aiolos represses CD4+ T cell cytotoxic programming via reciprocal regulation of TFH transcription factors and IL-2 sensitivity
    Authors: KA Read, DM Jones, S Pokhrel, EDS Hales, A Varkey, JA Tuazon, CD Eisele, O Abdouni, A Saadey, MR Leonard, RT Warren, MD Powell, JM Boss, EA Hemann, JS Yount, G Xin, HE Ghoneim, CJ Lio, AG Freud, PL Collins, KJ Oestreich
    Nature Communications, 2023-03-24;14(1):1652.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  6. Thymoquinone Inhibits JAK/STAT and PI3K/Akt/ mTOR Signaling Pathways in MV4-11 and K562 Myeloid Leukemia Cells
    Authors: Futoon Abedrabbu Al-Rawashde, Abdullah Saleh Al-wajeeh, Mansoureh Nazari Vishkaei, Hanan Kamel M. Saad, Muhammad Farid Johan, Wan Rohani Wan Taib et al.
    Pharmaceuticals (Basel)
  7. Whole‐genome analysis of TET dioxygenase function in regulatory T cells
    Authors: Xiaojing Yue, Daniela Samaniego‐Castruita, Edahí González‐Avalos, Xiang Li, Benjamin G Barwick, Anjana Rao
    EMBO reports
  8. Alprazolam Prompts HIV-1 Transcriptional Reactivation and Enhances CTL Response Through RUNX1 Inhibition and STAT5 Activation
    Authors: Angel Lin, Weam Othman Elbezanti, Alexis Schirling, Adel Ahmed, Rachel Van Duyne, Simon Cocklin et al.
    Frontiers in Neurology
  9. Deconvoluting global cytokine signaling networks in natural killer cells
    Authors: GM Wiedemann, EK Santosa, S Grassmann, S Sheppard, JB Le Luduec, NM Adams, C Dang, KC Hsu, JC Sun, CM Lau
    Nature Immunology, 2021-04-15;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  10. Tackling resistance in chronic myeloid leukemia: Novel cell death modulators with improved efficacy
    Authors: AM Schoepf, S Salcher, P Obexer, R Gust
    European Journal of Medicinal Chemistry, 2021-02-18;216(0):113285.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Lack of NFATc1 SUMOylation prevents autoimmunity and alloreactivity
    Authors: Yin Xiao, Musga Qureischi, Lena Dietz, Martin Vaeth, Subrahmanya D. Vallabhapurapu, Stefan Klein-Hessling et al.
    Journal of Experimental Medicine
  12. Divergent Role for STAT5 in the Adaptive Responses of Natural Killer Cells
    Authors: GM Wiedemann, S Grassmann, CM Lau, M Rapp, AV Villarino, C Friedrich, G Gasteiger, JJ O'Shea, JC Sun
    Cell Reports, 2020-12-15;33(11):108498.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ChIP, Flow Cytometry
  13. Ikaros antagonizes DNA binding by STAT5 in pre-B cells
    Authors: B Heizmann, S Le Gras, C Simand, P Marchal, S Chan, P Kastner
    PLoS ONE, 2020-11-12;15(11):e0242211.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  14. Benzodiazepines Drive Alteration of Chromatin at the Integrated HIV-1 LTR
    Authors: W Elbezanti, A Lin, A Schirling, A Jackson, M Marshall, RV Duyne, F Maldarelli, L Sardo, Z Klase
    Viruses, 2020-02-09;12(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  15. Elevated Prolactin during Pregnancy Drives a Phenotypic Switch in Mouse Hypothalamic Dopaminergic Neurons
    Authors: SH Yip, N Romanò, P Gustafson, DJ Hodson, EJ Williams, IC Kokay, AO Martin, P Mollard, DR Grattan, SJ Bunn
    Cell Rep, 2019-02-12;26(7):1787-1799.e5.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  16. Group 1 Innate Lymphoid Cell Lineage Identity Is Determined by a cis-Regulatory Element Marked by a Long Non-coding RNA
    Authors: Walter K. Mowel, Sam J. McCright, Jonathan J. Kotzin, Magalie A. Collet, Asli Uyar, Xin Chen et al.
    Immunity
  17. Phosphorylated STAT5 directly facilitates parvovirus B19 DNA replication in human erythroid progenitors through interaction with the MCM complex
    Authors: SS Ganaie, W Zou, P Xu, X Deng, S Kleiboeker, J Qiu
    PLoS Pathog., 2017-05-01;13(5):e1006370.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Identification of Novel STAT6-Regulated Proteins in Mouse B Cells by Comparative Transcriptome and Proteome Analysis
    Authors: L Mokada-Gop, A Boeser, CH Lehmann, F Drepper, D Dudziak, B Warscheid, D Voehringer
    J. Immunol, 2017-03-27;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. STAT5 Orchestrates Local Epigenetic Changes for Chromatin Accessibility and Rearrangements by Direct Binding to the TCRgamma Locus.
    Authors: Wagatsuma K, Tani-ichi S, Liang B, Shitara S, Ishihara K, Abe M, Miyachi H, Kitano S, Hara T, Nanno M, Ishikawa H, Sakimura K, Nakao M, Kimura H, Ikuta K
    J Immunol, 2015-07-20;195(4):1804-14.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ChIP
  20. Single-cell mass cytometry reveals intracellular survival/proliferative signaling in FLT3-ITD-mutated AML stem/progenitor cells
    Authors: Lina Han, Peng Qiu, Zhihong Zeng, Jeffrey L Jorgensen, Duncan H Mak, Jared K Burks et al.
    Cytometry Part A
  21. The TNF-family ligand TL1A and its receptor DR3 promote T cell-mediated allergic immunopathology by enhancing differentiation and pathogenicity of IL-9-producing T cells.
    Authors: Richard A, Tan C, Hawley E, Gomez-Rodriguez J, Goswami R, Yang X, Cruz A, Penumetcha P, Hayes E, Pelletier M, Gabay O, Walsh M, Ferdinand J, Keane-Myers A, Choi Y, O'Shea J, Al-Shamkhani A, Kaplan M, Gery I, Siegel R, Meylan F
    J Immunol, 2015-03-18;194(8):3567-82.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  22. Reversal of acquired drug resistance in FLT3-mutated acute myeloid leukemia cells via distinct drug combination strategies.
    Authors: Zhang W, Gao C, Konopleva M, Chen Y, Jacamo R, Borthakur G, Cortes J, Ravandi F, Ramachandran A, Andreeff M
    Clin Cancer Res, 2014-03-11;20(9):2363-74.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. Opposing actions of IL-2 and IL-21 on Th9 differentiation correlate with their differential regulation of BCL6 expression.
    Authors: Liao W, Spolski R, Li P, Du N, West E, Ren M, Mitra S, Leonard W
    Proc Natl Acad Sci U S A, 2014-02-18;111(9):3508-13.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ChIP
  24. Cutaneous T cell lymphoma expresses immunosuppressive CD80 (B7-1) cell surface protein in a STAT5-dependent manner.
    Authors: Zhang, Qian, Wang, Hong Yi, Wei, Fang, Liu, Xiaobin, Paterson, Jennifer, Roy, Darshan, Mihova, Daniela, Woetmann, Anders, Ptasznik, Andrzej, Odum, Niels, Schuster, Stephen, Marafioti, Teresa, Riley, James L, Wasik, Mariusz
    J Immunol, 2014-02-12;192(6):2913-9.
    Species: Human
    Sample Types: Cell Extracts, Cell Lysates
    Applications: ChIP, Functional Assay
  25. Human erythropoietin increases the pro-angiogenic potential of A2780 ovarian adenocarcinoma cells under hypoxic conditions
    Authors: JÁN KRIŠKA, PETER SOLÁR, LENKA VARINSKÁ, ZUZANA SOLÁROVÁ, PATRÍCIA KIMÁKOVÁ, JÁN MOJŽIŠ et al.
    Oncology Reports
  26. Location and the functionality of erythropoietin receptor(s) in A2780 cells
    Authors: Peter Solár, Gabriela Hrčková, Lenka Varinská, Zuzana Solárová, Ján Kriška, Ivana Uhrínová et al.
    Oncology Reports
  27. Growth factor independent-1 induced by IL-4 regulates Th2 cell proliferation.
    Authors: Zhu J, Guo L, Min B, Watson CJ, Hu-Li J, Young HA, Tsichlis PN, Paul WE
    Immunity, 2002-05-01;16(5):733-44.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. Thymoquinone Induces Downregulation of BCR-ABL/JAK/STAT Pathway and Apoptosis in K562 Leukemia Cells
    Authors: Al-Rawashde FA, Wan Taib WR, Ismail I et al.
    Asian Pacific journal of cancer prevention : APJCP

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Human/Mouse STAT5a/b Pan Specific Antibody
By Anonymous on 06/04/2019
Application: ChIP Sample Tested: Alzheimer's disease brain Species: Human