Home / SSEA-4 / Human/Mouse SSEA-4 PE-conjugated Antibody

Human/Mouse SSEA-4 PE-conjugated Antibody

Catalog #: FAB1435P
23 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1435P-100
FAB1435P-025
Detection of SSEA-4 in NTera-2 Human Cell Line by Flow Cytometry.
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Citations (23)
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Human/Mouse SSEA-4 PE-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Recognizes a carbohydrate epitope of SSEA-4 (1, 2).
Source
Monoclonal Mouse IgG3 Clone # MC-813-70
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
2120Ep human embryonal carcinoma cell line
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of SSEA-4 antibody in NTera-2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of SSEA-4 in NTera-2 Human Cell Line by Flow Cytometry. NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA-4 PE-conjugated Mono-clonal Antibody (Catalog # FAB1435P, filled histogram) or isotype control antibody (Catalog # IC007P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: SSEA-4

SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).

References
  1. Shevinsky, L.H. et al. (1982) Cell 30:697.
  2. Kannagi, R. et al. (1983) EMBO J. 2:2355.
  3. Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
  4. Draper, J.S. et al. (2002) J. Anat. 200:249.
Long Name
Stage-specific Embryonic Antigen-4
Alternate Names
SSEA4; SSEA-4; SSEA-4;stage-specific embryonic antigen 4

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Citations for Human/Mouse SSEA-4 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. A Novel Cell-Based Model for a Rare Disease: The Tks4-KO Human Embryonic Stem Cell Line as a Frank-Ter Haar Syndrome Model System
    Authors: L László, H Maczelka, T Takács, A Kurilla, Á Tilajka, L Buday, V Vas, Á Apáti
    International Journal of Molecular Sciences, 2022-08-08;23(15):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Patient-derived iPSCs link elevated mitochondrial respiratory complex I function to osteosarcoma in Rothmund-Thomson syndrome
    Authors: BE Jewell, A Xu, D Zhu, MF Huang, L Lu, M Liu, EL Underwood, JH Park, H Fan, JA Gingold, R Zhou, J Tu, Z Huo, Y Liu, W Jin, YH Chen, Y Xu, SH Chen, N Rainusso, NK Berg, DA Bazer, C Vellano, P Jones, HK Eltzschig, Z Zhao, BA Kaipparett, R Zhao, LL Wang, DF Lee
    PloS Genetics, 2021-12-29;17(12):e1009971.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  3. Generation of macrophages with altered viral sensitivity from genome-edited rhesus macaque iPSCs to model human disease
    Authors: Yoshihiro Iwamoto, Yohei Seki, Kahoru Taya, Masahiro Tanaka, Shoichi Iriguchi, Yasuyuki Miyake et al.
    Molecular Therapy - Methods & Clinical Development
  4. Generation of multiple iPSC clones from a male schizophrenia patient carrying de novo mutations in genes KHSRP, LRRC7, and KIR2DL1, and his parents
    Authors: E Hathy, E Szabó, K Vincze, I Haltrich, E Kiss, N Varga, Z Erdei, G Várady, L Homolya, Á Apáti, JM Réthelyi
    Stem Cell Research, 2020-12-25;51(0):102140.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  5. Highly efficient genome editing for single-base substitutions using optimized ssODNs with Cas9-RNPs
    Authors: S Okamoto, Y Amaishi, I Maki, T Enoki, J Mineno
    Sci Rep, 2019-03-18;9(1):4811.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. The Role of RNA Polymerase II Contiguity and Long-Range Interactions in the Regulation of Gene Expression in Human Pluripotent Stem Cells
    Authors: L Eiselleova, V Lukjanov, S Farkas, D Svoboda, K Stepka, I Koutna
    Stem Cells Int, 2019-02-03;2019(0):1375807.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Enhancing T Cell Receptor Stability in Rejuvenated iPSC-Derived T Cells Improves Their Use in Cancer Immunotherapy
    Authors: A Minagawa, T Yoshikawa, M Yasukawa, A Hotta, M Kunitomo, S Iriguchi, M Takiguchi, Y Kassai, E Imai, Y Yasui, Y Kawai, R Zhang, Y Uemura, H Miyoshi, M Nakanishi, A Watanabe, A Hayashi, K Kawana, T Fujii, T Nakatsura, S Kaneko
    Cell Stem Cell, 2018-11-15;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. A Marfan syndrome human induced pluripotent stem cell line with a heterozygous FBN1 c.4082G>A mutation, ISMMSi002-B, for disease modeling
    Authors: S Klein, JL Dvornik, AR Yarrabothu, C Schaniel
    Stem Cell Res, 2017-07-03;23(0):73-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. An assessment of the effects of ectopic gp91phox expression in XCGD iPSC-derived neutrophils
    Authors: Huan-Ting Lin, Hideki Masaki, Tomoyuki Yamaguchi, Taizo Wada, Akihiro Yachie, Ken Nishimura et al.
    Molecular Therapy - Methods & Clinical Development
  10. An alternative pluripotent state confers interspecies chimaeric competency.
    Authors: Wu J, Okamura D, Li M, Suzuki K, Luo C, Ma L, He Y, Li Z, Benner C, Tamura I, Krause M, Nery J, Du T, Zhang Z, Hishida T, Takahashi Y, Aizawa E, Kim N, Lajara J, Guillen P, Campistol J, Esteban C, Ross P, Saghatelian A, Ren B, Ecker J, Izpisua Belmonte J
    Nature, 2015-05-06;521(7552):316-21.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Characterization of Cell Subpopulations Expressing Progenitor Cell Markers in Porcine Cardiac Valves
    Authors: Huan Wang, Balaji Sridhar, Leslie A. Leinwand, Kristi S. Anseth
    PLoS ONE
  12. The C-terminus of tissue factor pathway inhibitor alpha is required for its interaction with factors V and Va.
    J. Thromb. Haemost., 2012-09-01;10(9):1944-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. GMP-compliant isolation and large-scale expansion of bone marrow-derived MSC.
    Authors: Fekete N, Rojewski M, Furst D, Kreja L, Ignatius A, Dausend J, Schrezenmeier H
    PLoS ONE, 2012-08-14;7(8):e43255.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Cross-presentation of tumour antigens by human induced pluripotent stem cell-derived CD141(+)XCR1+ dendritic cells.
    Gene Ther, 2011-11-10;19(10):1035-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Human dermis harbors distinct mesenchymal stromal cell subsets.
    Authors: Vaculik C, Schuster C, Bauer W, Iram N, Pfisterer K, Kramer G, Reinisch A, Strunk D, Elbe-Burger A
    J. Invest. Dermatol., 2011-11-03;132(3):563-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Brief report: benchmarking human pluripotent stem cell markers during differentiation into the three germ layers unveils a striking heterogeneity: all markers are not equal.
    Stem Cells, 2011-09-01;29(9):1469-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Subpopulations of Human Embryonic Stem Cells With Distinct Tissue-Specific Fates Can Be Selected From Pluripotent Cultures
    Authors: Frank W. King, Carissa Ritner, Walter Liszewski, Helen C.K. Kwan, Anissa Pedersen, Andrew D. Leavitt et al.
    Stem Cells and Development
  18. Efficient, high-throughput transfection of human embryonic stem cells
    Authors: Jennifer C Moore, Kristin Atze, Percy L Yeung, Alana J Toro-Ramos, Cynthia Camarillo, Kevin Thompson et al.
    Stem Cell Research & Therapy
  19. Patient-specific induced pluripotent stem-cell-derived models of LEOPARD syndrome.
    Authors: Carvajal-Vergara X, Sevilla A, D'Souza SL
    Nature, 2010-06-10;465(7299):808-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. Multipotent mesenchymal stem cells from human placenta: critical parameters for isolation and maintenance of stemness after isolation.
    Authors: Semenov O, Koestenbauer S, Riegel M, Zech N, Zimmermann R, Zisch A, Malek A
    Am J Obstet Gynecol, 2009-12-24;202(2):193.e1-193.e1.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  21. Expression of an exogenous human Oct-4 promoter identifies tumor-initiating cells in osteosarcoma.
    Authors: Levings PP, McGarry SV, Currie TP, Nickerson DM, McClellan S, Ghivizzani SC, Steindler DA, Gibbs CP
    Cancer Res., 2009-07-07;69(14):5648-55.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Human embryonic stem cells in culture possess primary cilia with hedgehog signaling machinery.
    Authors: Kiprilov EN, Awan A, Desprat R, Velho M, Clement CA, Byskov AG, Andersen CY, Satir P, Bouhassira EE, Christensen ST, Hirsch RE
    J. Cell Biol., 2008-03-10;180(5):897-904.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  23. T lineage differentiation from human embryonic stem cells.
    Authors: Galic Z, Kitchen SG, Kacena A, Subramanian A, Burke B, Cortado R, Zack JA
    Proc. Natl. Acad. Sci. U.S.A., 2006-07-14;103(31):11742-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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