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Human/Mouse SSEA-1 PE-conjugated Antibody

Catalog #: FAB2155P
14 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB2155P-100
FAB2155P-025
Detection of SSEA‑1 in D3 Mouse Cell Line by Flow Cytometry.
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Citations (14)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse SSEA-1 PE-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse SSEA-1.
Source
Monoclonal Mouse IgM Clone # MC-480
Purification
IgM-specific Affinity-purified from hybridoma culture supernatant
Immunogen
F9 mouse teratocarcinoma stem cells
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µL/106 cells
D3 mouse embryonic stem cell line and Whole blood granulocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of SSEA-1 antibody in D3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of SSEA‑1 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Mouse Anti-Human/Mouse SSEA-1 PE-conjugated Monoclonal Antibody (Catalog # FAB2155P, filled histogram) or isotype control antibody (IC015P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of SSEA‑1 in Whole blood granulocytes cells by Flow Cytometry. Whole blood granulocytes cells were stained with Mouse Anti-Human/Mouse SSEA‑1 PE‑conjugated Monoclonal Antibody (Catalog # FAB2155P, filled histogram) or isotype control antibody (Catalog # IC015P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: SSEA-1

Stage-Specific Embryonic Antigen-1 (SSEA-1), an antigenic epitope defined as a Lewis x carbohydrate structure, is expressed during early mouse embryogenesis on murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES), and murine and human germ cells. Expression of SSEA-1 is down-regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression (1, 2).

References
  1. Solter, D. and Knowles, B.B. (1978) Proc. Natl. Acad. Sci. USA 75:5565.
  2. Fox, N. et al. (1983) Cancer Res. 43:669.
Long Name
Stage-specific Embryonic Antigen-1
Alternate Names
alpha-(1,3)-fucosyltransferase; CD15; EC 2.4.1; EC 2.4.1.-; EC 2.4.1.65; ELAM-1 ligand fucosyltransferase; ELFT; FCT3AELAM ligand fucosyltransferase; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase 4; Fucosyltransferase IV; FUC-TIV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; LeX; SSEA1; SSEA-1; stage-specific embryonic antigen 1

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Citations for Human/Mouse SSEA-1 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Expression of the cancer stem cell marker SSEA1 is associated with poor survival in metastatic high-grade serous carcinoma
    Authors: Ben Davidson, Arild Holth, Hiep Phuc Dong
    Virchows Archiv
  2. A dual molecular analogue tuner for dissecting protein function in mammalian cells
    Nat Commun, 2016-05-27;7(0):11742.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Human primordial germ cell commitment in vitro associates with a unique PRDM14 expression profile.
    Authors: Sugawa F, Arauzo-Bravo M, Yoon J, Kim K, Aramaki S, Wu G, Stehling M, Psathaki O, Hubner K, Scholer H
    EMBO J, 2015-03-06;34(8):1009-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. SOX9 accelerates ESC differentiation to three germ layer lineages by repressing SOX2 expression through P21 (WAF1/CIP1)
    Authors: Kohei Yamamizu, David Schlessinger, Minoru S. H. Ko
    Development
  5. 5-Aminoimidazole-4-carboxyamide ribonucleoside induces G(1)/S arrest and Nanog downregulation via p53 and enhances erythroid differentiation.
    Authors: Chae HD, Lee MR, Broxmeyer HE
    Stem Cells, 2012-02-01;30(2):140-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Human Periosteum Is a Source of Cells for Orthopaedic Tissue Engineering: A Pilot Study
    Authors: Michael D. Ball, Ian C. Bonzani, Melissa J. Bovis, Andrew Williams, Molly M. Stevens
    Clinical Orthopaedics & Related Research
  7. Foxm1 transcription factor is required for maintenance of pluripotency of P19 embryonal carcinoma cells
    Authors: Zhongqiu Xie, Guixiang Tan, Miao Ding, Difei Dong, Tuanhui Chen, Xiangxian Meng et al.
    Nucleic Acids Research
  8. A defined glycosaminoglycan-binding substratum for human pluripotent stem cells.
    Authors: Klim JR, Li L, Wrighton PJ
    Nat. Methods, 2010-11-14;7(12):989-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Interwoven Four-Compartment Capillary Membrane Technology for Three-Dimensional Perfusion with Decentralized Mass Exchange to Scale Up Embryonic Stem Cell Culture
    Authors: Jörg C. Gerlach, Marc Lübberstedt, Josefina Edsbagge, Alexander Ring, Mariah Hout, Matt Baun et al.
    Cells Tissues Organs
  10. Self-renewing epiblast stem cells exhibit continual delineation of germ cells with epigenetic reprogramming in vitro.
    Authors: Hayashi K, Surani MA
    Development, 2009-09-30;136(21):3549-56.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  11. Automated, scalable culture of human embryonic stem cells in feeder-free conditions.
    Authors: Thomas RJ, Anderson D, Chandra A, Smith NM, Young LE, Williams D, Denning C
    Biotechnol. Bioeng., 2009-04-15;102(6):1636-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Direct reprogramming of terminally differentiated mature B lymphocytes to pluripotency.
    Authors: Hanna J, Markoulaki S, Schorderet P, Carey BW, Beard C, Wernig M, Creyghton MP, Steine EJ, Cassady JP, Foreman R, Lengner CJ, Dausman JA, Jaenisch R
    Cell, 2008-04-18;133(2):250-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. Regulation of self-renewal and pluripotency by Sox2 in human embryonic stem cells.
    Authors: Fong H, Hohenstein KA, Donovan PJ
    Stem Cells, 2008-04-03;26(8):1931-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Kidney tissue reconstruction by fetal kidney cell transplantation: effect of gestation stage of fetal kidney cells.
    Authors: Kim SS, Gwak SJ, Han J, Park HJ, Park MH, Song KW, Cho SW, Rhee YH, Chung HM, Kim BS
    Stem Cells, 2007-06-01;25(6):1393-401.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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