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Human/Mouse SSEA-1 Antibody

Catalog #: MAB2155
22 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2155
MAB2155-SP
SSEA‑1 in D3 Mouse Cell Line.
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Product Details
Citations (22)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse SSEA-1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse SSEA-1.
Source
Monoclonal Mouse IgM Clone # MC-480
Purification
IgM-specific Affinity-purified from hybridoma culture supernatant
Immunogen
F9 mouse teratocarcinoma stem cells
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
Whole blood granulocytes
Immunoprecipitation
Ballou, B. et al. (1979) Science 206:844.
 
CyTOF-reported
Lujan, E. et al. (2015) Nature 521: 352. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry SSEA‑1 antibody in D3 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

SSEA‑1 in D3 Mouse Cell Line. SSEA‑1 was detected in immersion fixed D3 mouse embryonic stem cell line using 10 µg/mL Human/Mouse SSEA‑1 Monoclonal Antibody (Catalog # MAB2155) for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Flow Cytometry View Larger

Detection of SSEA‑1 in Whole blood granulocytes by Flow Cytometry. Whole blood granulocytes cells were stained with Mouse Anti-Human/Mouse SSEA‑1 Monoclonal Antibody (Catalog # MAB2155, filled histogram) or isotype control antibody (open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0117). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry/ Immunofluorescence Detection of Mouse SSEA-1 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse SSEA-1 by Immunocytochemistry/Immunofluorescence Characteristics of mouse embryonic stem cells (mESCs) cultivated in 2i, R2i, and serum. Alkaline phosphatase (ALP) staining (scale bar: 100 µm) and immunofluorescence (IF) labeling for Oct4, SSEA-1, and Nanog counterstained for DAPI are shown (scale bar: 50 µm). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29845793), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Canine SSEA-1 by Flow Cytometry View Larger

Detection of Canine SSEA-1 by Flow Cytometry Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-8-150), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Canine SSEA-1 by Flow Cytometry View Larger

Detection of Canine SSEA-1 by Flow Cytometry Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-8-150), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Human/Mouse SSEA-1 Antibody by Immunocytochemistry/ Immunofluorescence View Larger

Detection of Mouse Human/Mouse SSEA-1 Antibody by Immunocytochemistry/ Immunofluorescence Characteristics of mouse embryonic stem cells (mESCs) cultivated in 2i, R2i, and serum. Alkaline phosphatase (ALP) staining (scale bar: 100 µm) and immunofluorescence (IF) labeling for Oct4, SSEA-1, and Nanog counterstained for DAPI are shown (scale bar: 50 µm). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29845793), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Canine Human/Mouse SSEA-1 Antibody by Flow Cytometry View Larger

Detection of Canine Human/Mouse SSEA-1 Antibody by Flow Cytometry Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22937862), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Canine Human/Mouse SSEA-1 Antibody by Flow Cytometry View Larger

Detection of Canine Human/Mouse SSEA-1 Antibody by Flow Cytometry Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22937862), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SSEA-1

Stage-Specific Embryonic Antigen-1 (SSEA-1), an antigenic epitope defined as a Lewis x carbohydrate structure, is expressed during early mouse embryogenesis on murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES), and murine and human germ cells. Expression of SSEA-1 is down-regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression (1, 2).

References
  1. Solter, D. and Knowles, B.B. (1978) Proc. Natl. Acad. Sci. USA 75:5565.
  2. Fox, N. et al. (1983) Cancer Res. 43:669.
Long Name
Stage-specific Embryonic Antigen-1
Alternate Names
alpha-(1,3)-fucosyltransferase; CD15; EC 2.4.1; EC 2.4.1.-; EC 2.4.1.65; ELAM-1 ligand fucosyltransferase; ELFT; FCT3AELAM ligand fucosyltransferase; fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific); Fucosyltransferase 4; Fucosyltransferase IV; FUC-TIV; fucT-IV; FUTIV; Galactoside 3-L-fucosyltransferase; Lewis X; LeX; SSEA1; SSEA-1; stage-specific embryonic antigen 1

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Citations for Human/Mouse SSEA-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. Cloning, expression, and functional characterization of in-house prepared human leukemia inhibitory factor
    Authors: Hassan Rassouli, Shiva Nemati, Siamak Rezaeiani, Ali Sayadmanesh, Mohammad Reza Gharaati, Ghasem Hosseini Hosseini Salekdeh et al.
    Cell J
  2. Transcriptomic analysis of feeder-free culture system for maintaining naïve-state pluripotency in human pluripotent stem cells
    Authors: Wataru Isono, Tomoyuki Kawasaki, Justin K. Ichida, Kazunori Nagasaka, Osamu Hiraike, Akihiro Umezawa et al.
    Stem Cell Investigation
  3. A secreted proteomic footprint for stem cell pluripotency
    Authors: Lewis, PA;Silajdži?, E;Smith, H;Bates, N;Smith, CA;Mancini, FE;Knight, D;Denning, C;Brison, DR;Kimber, SJ;
    PloS one
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  4. The Use of Trichostatin A during Pluripotent Stem Cell Generation Does Not Affect MHC Expression Level
    Authors: S Farahi, S Hosseini, H Ghanbarian, SM Hashemi, M Salehi, S Hosseini
    Stem Cells International, 2022-02-15;2022(0):9346767.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC/IF
  5. The H2B ubiquitin-protein ligase RNF40 is required for somatic cell reprogramming
    Authors: W Xie, M Miehe, S Laufer, SA Johnsen
    Cell Death Dis, 2020-04-27;11(4):287.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  6. Phospholipid remodeling is critical for stem cell pluripotency by facilitating mesenchymal-to-epithelial transition
    Authors: Y Wu, K Chen, G Xing, L Li, B Ma, Z Hu, L Duan, X Liu
    Sci Adv, 2019-11-27;5(11):eaax7525.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  7. TRIM28 epigenetic corepressor is indispensable for stable induced pluripotent stem cell formation
    Authors: M Klimczak, P Czerwi?ska, S Mazurek, B Soza?ska, P Biecek, A Mackiewicz, M Wiznerowic
    Stem Cell Res, 2017-07-15;23(0):163-172.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Metabolic Reprogramming, Autophagy, and Reactive Oxygen Species Are Necessary for Primordial Germ Cell Reprogramming into Pluripotency
    Authors: D Sainz de l, A Moratilla, V Aparicio, C Lorca, Y Alcaina, D Martín, MP De Miguel
    Oxid Med Cell Longev, 2017-07-05;2017(0):4745252.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  9. HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia
    Authors: P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu
    Oncotarget, 2017-04-25;8(17):28074-28092.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  10. Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience
    Authors: PA De Sousa, R Steeg, E Wachter, K Bruce, J King, M Hoeve, S Khadun, G McConnachi, J Holder, A Kurtz, S Seltmann, J Dewender, S Reimann, G Stacey, O O'Shea, C Chapman, L Healy, H Zimmermann, B Bolton, T Rawat, I Atkin, A Veiga, B Kuebler, BM Serano, T Saric, J Hescheler, O Brüstle, M Peitz, C Thiele, N Geijsen, B Holst, C Clausen, M Lako, L Armstrong, SK Gupta, AJ Kvist, R Hicks, A Jonebring, G Brolén, A Ebneth, A Cabrera-So, P Foerch, M Geraerts, TC Stummann, S Harmon, C George, I Streeter, L Clarke, H Parkinson, PW Harrison, A Faulconbri, L Cherubin, T Burdett, C Trigueros, MJ Patel, C Lucas, B Hardy, R Predan, J Dokler, M Brajnik, O Keminer, O Pless, P Gribbon, C Claussen, A Ringwald, B Kreisel, A Courtney, TE Allsopp
    Stem Cell Res, 2017-03-07;20(0):105-114.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Pontin functions as an essential coactivator for Oct4-dependent lincRNA expression in mouse embryonic stem cells.
    Authors: Boo, Kyungjin, Bhin, Jinhyuk, Jeon, Yoon, Kim, Joomyung, Shin, Hi-Jai R, Park, Jong-Eun, Kim, Kyeongky, Kim, Chang Ro, Jang, Hyonchol, Kim, In-Hoo, Kim, V Narry, Hwang, Daehee, Lee, Ho, Baek, Sung Hee
    Nat Commun, 2015-04-10;6(0):6810.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  12. CXCR2 and its related ligands play a novel role in supporting the pluripotency and proliferation of human pluripotent stem cells.
    Authors: Jung J, Lee S, Kim J, Lee S, Sung H, An J, Park Y, Kim B
    Stem Cells Dev, 2014-12-23;24(8):948-61.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. B-cell receptor-associated protein 31 regulates human embryonic stem cell adhesion, stemness, and survival via control of epithelial cell adhesion molecule.
    Authors: Kim W, Seo Choi H, Min Lee H, Jang Y, Ryu C
    Stem Cells, 2014-10-01;32(10):2626-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Pluripotent stem cells derived from mouse and human white mature adipocytes.
    Authors: Jumabay M, Abdmaulen R, Ly A, Cubberly M, Shahmirian L, Heydarkhan-Hagvall S, Dumesic D, Yao Y, Bostrom K
    Stem Cells Transl Med, 2014-01-06;3(2):161-71.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  15. Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells.
    BMC Vet Res, 2012-08-31;8(0):150.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Tumour vascularization via endothelial differentiation of glioblastoma stem-like cells.
    Authors: Ricci-Vitiani L, Pallini R, Biffoni M, Todaro M, Invernici G, Cenci T, Maira G, Parati EA, Stassi G, Larocca LM, De Maria R
    Nature, 2010-11-21;468(7325):824-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  17. Aire regulates the expression of differentiation-associated genes and self-renewal of embryonic stem cells.
    Authors: Gu B, Zhang J, Chen Q, Tao B, Wang W, Zhou Y, Chen L, Liu Y, Zhang M
    Biochem. Biophys. Res. Commun., 2010-03-10;394(2):418-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  18. Equine umbilical cord blood contains a population of stem cells that express Oct4 and differentiate into mesodermal and endodermal cell types.
    Authors: Reed SA, Johnson SE
    J. Cell. Physiol., 2008-05-01;215(2):329-36.
    Species: Equine
    Sample Types: Whole Cells
    Applications: ICC
  19. Guided differentiation of embryonic stem cells into Pdx1-expressing regional-specific definitive endoderm.
    Authors: Shiraki N, Yoshida T, Araki K, Umezawa A, Higuchi Y, Goto H, Kume K, Kume S
    Stem Cells, 2008-01-31;26(4):874-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  20. The human tumor antigen PRAME is a dominant repressor of retinoic acid receptor signaling.
    Authors: Epping MT, Wang L, Edel MJ, Carlee L, Hernandez M, Bernards R
    Cell, 2005-09-23;122(6):835-47.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  21. Generation of Human‐Induced Pluripotent Stem Cells From Anterior Cruciate Ligament
    Authors: Steven Woods, Nicola Bates, Sara L. Dunn, Ferdinand Serracino‐Inglott, Tim E. Hardingham, Susan J. Kimber
    Journal of Orthopaedic Research

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Human/Mouse SSEA-1 Antibody
By Julieta O'Flaherty on 12/11/2020
Application: Immunocytochemistry/Immunofluorescence Sample Tested: iPS2 human induced pluripotent stem cells Species: Human

I used to check whether SSEA-1 is expressed in my iPSCs and it was great.. no background signal

Immunocytochemistry/Immunofluorescence Human/Mouse SSEA-1 Antibody MAB2155