Human/Mouse ROR gamma t/RORC2/NR1F3 APC-conjugated Antibody
Human/Mouse ROR gamma t/RORC2/NR1F3 APC-conjugated Antibody Summary
Met1-Arg10
Accession # P51449
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of ROR gamma t/RORC2/NR1F3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PMA, Calcium Ionomycin, LPS, and Recombinant Human IL-23 (Catalog # 1290-IL) were stained with Mouse Anti-Human/Mouse ROR gammat/RORC2/NR1F3 APC-conjugated Monoclonal Antibody (Catalog # IC6006A) and Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # IC0041A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Detection of ROR gamma t/RORC2/NR1F3 in Mouse CD4+/CD8+Thymocytes by Flow Cytometry. Mouse CD4+/CD8+thymocytes were stained with Mouse Anti-Human/Mouse ROR gammat/RORC2/NR1F3 APC-conjugated Monoclonal Antibody (Catalog # IC6006A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ROR gamma t/RORC2/NR1F3
Retinoic acid-related Orphan Receptor gamma (ROR gamma, TOR, RORC; NR1F3) is a member of the orphan nuclear receptor family. ROR gamma is expressed in the muscle, thymus, testis, pancreas, prostate, heart, and liver. ROR gamma plays a role in thymocyte development and homeostasis. RORs bind to DNA as monomers on half-site elements with 5' A/T-rich extensions. An N-terminal isoform of ROR gamma, ROR gamma t, has been shown to be specifically expressed in the thymus.
Product Datasheets
Citations for Human/Mouse ROR gamma t/RORC2/NR1F3 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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More fuel to the fire: some patients with non-celiac self-reported wheat sensitivity exhibit adaptive immunological responses in duodenal mucosa
Authors: AI Castillo-R, J Furuzawa-C, M Peláez-Lun, J Castro-Góm, Y López-Vida, L Uscanga
BMC gastroenterology, 2020-12-09;20(1):414.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Leukocyte function-associated antigen-1/intercellular adhesion molecule-1 interaction induces a novel genetic signature resulting in T-cells refractory to transforming growth factor-beta signaling.
Authors: Verma N, Dempsey E, Long A, Davies A, Barry S, Fallon P, Volkov Y, Kelleher D
J Biol Chem, 2012-06-15;287(32):27204-16.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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