Home / PKA C alpha/beta / Human/Mouse/Rat PKA C alpha / beta Antibody

Human/Mouse/Rat PKA C alpha / beta Antibody

Catalog #: MAB5908
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB5908
MAB5908-SP
Detection of Human/Mouse/Rat PKA C alpha / beta by Western Blot.
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Product Details
Citations (4)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat PKA C alpha / beta Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat PKA C alpha / beta in Western blots.
Source
Monoclonal Mouse IgG2A Clone # 515741
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PKA C alpha
Met1-Phe351
Accession # P17612
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat PKA Ca/ beta antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat PKA C alpha / beta by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat PKA Ca/ beta Monoclonal Antibody (Catalog # MAB5908) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for PKA Ca/ beta was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Western Blot Detection of Human PKA Ca/ beta antibody by Western Blot. View Larger

Detection of Human PKA C alpha / beta by Western Blot. Western blot shows Recombinant Human Active PKA Ca (Catalog # 4268-KS), Recombinant Human Active PKA C beta (Catalog # 4596-KS), and recombinant human PKA C gamma (1 ng/lane each). PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat PKA Ca/ beta Monoclonal Antibody (Catalog # MAB5908) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PKA C alpha/beta

The cAMP-dependent protein kinase (PKA) holoenzyme is composed of two regulatory and two catalytic subunits, designated PKA R and PKA C, respectively. Three catalytic subunit isoforms, C alpha, C beta, and C gamma, have been identified. Upon PKA R subunit binding to the second messenger cAMP, active PKA C subunits are released, initiating a phosphorylation cascade that regulates such cellular functions as metabolism, ion transport, and gene transcription.

Long Name
cAMP-dependent Protein Kinase Catalytic Subunit alpha/beta
Entrez Gene IDs
5566 (Human); 18747 (Mouse); 25636 (Rat)
Alternate Names
CAFD2; PKA C alpha/beta; PKA C-beta; PKACB; PRKACA/B

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Citations for Human/Mouse/Rat PKA C alpha / beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Anti-neoplastic action of Cimetidine/Vitamin C on histamine and the PI3K/AKT/mTOR pathway in Ehrlich breast cancer
    Authors: Sherihan Salaheldin Abdelhamid Ibrahim, Sarah A. Abd El-Aal, Ahmed M. Reda, Samar El Achy, Yasmine Shahine
    Scientific Reports
  2. Protein phosphatase 2A regulatory subunit B56 alpha limits phosphatase activity in the heart
    Authors: Sean C. Little, Jerry Curran, Michael A. Makara, Crystal F. Kline, Hsiang-Ting Ho, Zhaobin Xu et al.
    Science Signaling
  3. Prostaglandin E3 attenuates macrophage-associated inflammation and prostate tumour growth by modulating polarization
    Authors: J Cui, K Shan, Q Yang, Y Qi, H Qu, J Li, R Wang, L Jia, W Chen, N Feng, YQ Chen
    Journal of Cellular and Molecular Medicine, 2021-05-13;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. cAMP initiates early phase neuron-like morphology changes and late phase neural differentiation in mesenchymal stem cells.
    Authors: Zhang L, Seitz LC, Abramczyk AM
    Cell. Mol. Life Sci., 2010-08-20;68(5):863-76.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot

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