Human/Mouse/Rat PI 3-Kinase p55 gamma Antibody

Catalog #: AF5734
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5734
AF5734-SP

Detection of Human/Mouse/Rat PI 3-Kinase p55 gamma by Western Blot.

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Human/Mouse/Rat PI 3-Kinase p55 gamma Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse and rat PI 3-Kinase p55 gamma in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PI 3-Kinase p55 gamma
Lys251-Gly378
Accession # Q92569

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human/Mouse/Rat PI 3-Kinase p55 gamma by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, C2C12 mouse myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PI 3-Kinase p55 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5734) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PI 3-Kinase p55 gamma at approximately 55 to 57 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PI 3-Kinase p55 gamma

p55 gamma (Protein of 55 kDa, gamma subunit; also PI 3-Kinase regulatory subunit gamma and p55^PIK) is a 55-57 kDa member of the PI 3K p85 family of regulatory subunits. It is widely expressed and serves as a recruitment subunit for the p110 catalytic subunit of PI 3-Kinase. p55 gamma /p55^PIK should not be confused with the 55 kDa alpha splice variant of the p85 alpha gene (75% amino acid [aa] identity). Human p55 gamma is 461 aa in length and contains a unique 34 aa N-terminus that binds Rb and prevents cycle progression, followed by a Pro-rich region (aa 35-44) and two SH2 domains that bind the p110 catalytic subunit (aa 65-160 and 358-452).

Long Name

PI 3-Kinase p55 gamma

Entrez Gene IDs

8503 (Human); 18710 (Mouse); 60664 (Rat)

Alternate Names

DKFZp686P05226; FLJ41892; p55; p55-GAMMA; p55PIK; Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; phosphatidylinositol 3-kinase regulatory subunit gamma; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 3 (p55, gamma); phosphoinositide-3-kinase, regulatory subunit 3 (gamma); PI 3Kinase p55 gamma; PI 3-Kinase p55 gamma; PI3K regulatory subunit gamma; PI3-kinase regulatory subunit gamma; PI3-kinase subunit p55-gamma; PIK3R3; PtdIns-3-kinase regulatory subunit gamma; ptdIns-3-kinase regulatory subunit p55-gamma; regulatory subunit, polypeptide 3 (p55, gamma)

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Citation for Human/Mouse/Rat PI 3-Kinase p55 gamma Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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