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Human/Mouse/Rat Phospho-Chk1 (S317) Antibody

Catalog #: AF2054
10 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2054
AF2054-SP
Detection of Human Phospho-Chk1 (S317) by Western Blot.
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Product Details
Citations (10)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Phospho-Chk1 (S317) Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Chk1 when phosphorylated at S317 in Western blots. Does not recognize Chk1 when unphosphorylated at S317.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human Chk1 S317 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Phospho-Chk1 (S317) antibody by Western Blot. View Larger

Detection of Human Phospho-Chk1 (S317) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or exposed (+) to 50 J/m2UV-C for the indicated time. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human/Mouse/Rat Phospho-Chk1 (S317) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2054) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-Chk1 (S317) was detected at approximately 56 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Phospho-Chk1 (S317) antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human Phospho-Chk1 (S317) by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 50 J/m2ultraviolet light (UV) for 2 hours, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Chk1 (S317) at approximately 60 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Chk1 (S317) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2054). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chk1

The Chk1 checkpoint kinase is an integral member of a signaling cascade that controls cell cycle progression. In response to genotoxic or replicative stress, Chk1 is phosphorylated by ATM or ATM-related kinases (ATR) at S317. In turn, Chk1 phosphorylates downstream effectors, such as p53 or the Cdc25 phosphatases to halt cell cycle progression and allow time for repair of incurred damage.

Long Name
Checkpoint Kinase 1
Entrez Gene IDs
1111 (Human); 12649 (Mouse); 140583 (Rat)
Alternate Names
Checkpoint, S. pombe, homolog of, 1; CHEK1; CHK1 (checkpoint, S.pombe) homolog; CHK1 checkpoint homolog (S. pombe); Chk1; CHK1serine/threonine-protein kinase Chk1; EC 2.7.11; EC 2.7.11.1

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Citations for Human/Mouse/Rat Phospho-Chk1 (S317) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Establishment and Characterization of Multi-Drug Resistant p53-Negative Osteosarcoma SaOS-2 Subline
    Authors: Boichuk S, Bikinieva F, Valeeva E et al.
    Diagnostics (Basel)
  2. HERC2 regulates RPA2 by mediating ATR-induced Ser33 phosphorylation and ubiquitin-dependent degradation
    Authors: Y Lai, M Zhu, W Wu, N Rokutanda, Y Togashi, W Liang, T Ohta
    Sci Rep, 2019-10-03;9(1):14257.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. miR146a-mediated targeting of FANCM during inflammation compromises genome integrity
    Authors: Devakumar Sundaravinayagam, Hye Rim Kim, TingTing Wu, Hyun Hee Kim, Hyun-Seo Lee, Semo Jun et al.
    Oncotarget
  4. PIG3 Functions in DNA Damage Response through Regulating DNA-PKcs Homeostasis
    Authors: Bing Li, Zeng-Fu Shang, Jiao-Jiao Yin, Qin-Zhi Xu, Xiao-Dan Liu, Yu Wang et al.
    International Journal of Biological Sciences
  5. Dbf4 is direct downstream target of ataxia telangiectasia mutated (ATM) and ataxia telangiectasia and Rad3-related (ATR) protein to regulate intra-S-phase checkpoint.
    Authors: Lee AY, Chiba T, Truong LN
    J. Biol. Chem., 2011-11-28;287(4):2531-43.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Functional connection between Rad51 and PML in homology-directed repair.
    Authors: Boichuk S, Hu L, Makielski K, Pandolfi PP, Gjoerup OV
    PLoS ONE, 2011-10-05;6(10):e25814.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Poly(ADP-Ribose) polymerase inhibition synergizes with 5-fluorodeoxyuridine but not 5-fluorouracil in ovarian cancer cells.
    Authors: Huehls AM, Wagner JM, Huntoon CJ, Geng L, Erlichman C, Patel AG, Kaufmann SH, Karnitz LM
    Cancer Res., 2011-05-25;71(14):4944-54.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Multiple DNA damage signaling and repair pathways deregulated by simian virus 40 large T antigen.
    Authors: Boichuk S, Hu L, Hein J
    J. Virol., 2010-06-02;84(16):8007-20.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Simian virus 40 large T antigen disrupts genome integrity and activates a DNA damage response via Bub1 binding.
    Authors: Hein J, Boichuk S, Wu J, Cheng Y, Freire R, Jat PS, Roberts TM, Gjoerup OV
    J. Virol., 2008-10-15;83(1):117-27.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  10. RNF8-dependent and RNF8-independent regulation of 53BP1 in response to DNA damage.
    Authors: Sakasai R, Tibbetts R
    J. Biol. Chem., 2008-03-12;283(20):13549-55.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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