Human/Mouse/Rat p27/Kip1 Antibody Summary
Met1-Thr198
Accession # P46527
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human p27/Kip1 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human p27/Kip1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2256) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for p27/Kip1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of p27/Kip1in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells were stained with Goat Anti-Human p27/Kip1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2256, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p27/Kip1
The cyclin-dependent kinase (cdk) inhibitor p27/Kip1 is a tumor suppressor protein that acts in the nucleus to enforce cell cycle checkpoints. p27/Kip1 binds to and inhibits many cyclin/cdk complexes, including cyclin D/cdk4, cyclin E/cdk2, and cyclin A/cdk2. This inhibition can subsequently block progression through different phases of the cell cycle.
Product Datasheets
Citations for Human/Mouse/Rat p27/Kip1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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The E3 ligase SMURF1 stabilizes p27 via UbcH7 catalyzed K29-linked ubiquitin chains to promote cell migration SMURF1-UbcH7 K29 ubiquitination of p27 and cell migration
Authors: Weinberg, J;Whitcomb, E;Bohm, A;Chekkilla, UK;Taylor, A;
The Journal of biological chemistry
Species: Human
Sample Types: Whole Cells
Applications: Pull Down Assay -
Cytoplasmic sequestration of p27 via AKT phosphorylation in renal cell carcinoma.
Authors: Kim J, Jonasch E, Alexander A, Short JD, Cai S, Wen S, Tsavachidou D, Tamboli P, Czerniak BA, Do KA, Wu KJ, Marlow LA, Wood CG, Copland JA, Walker CL
Clin. Cancer Res., 2009-01-01;15(1):81-90.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
IL-12-dependent innate immunity arrests endothelial cells in G0-G1 phase by a p21(Cip1/Waf1)-mediated mechanism
Authors: Lucia Napione, Marina Strasly, Claudia Meda, Stefania Mitola, Maria Alvaro, Gabriella Doronzo et al.
Angiogenesis
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