Human/Mouse/Rat JNK1 Antibody Summary
Ser2-Ile384
Accession # P45983-1
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of Human JNK1 antibody by Western Blot. Detection of Human JNK1 antibody by Western Blot.](https://resources.rndsystems.com/images/datasheets/antibody/JNK1_MAB17761_Western_Blot_6327.jpg)
Detection of Human JNK1 by Western Blot. Western blot shows recombinant human JNK1, JNK2, and JNK3 (1 ng/lane). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for JNK1 was detected at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
![Detection of Human, Mouse, and Rat JNK1 antibody by Western Blot. Detection of Human, Mouse, and Rat JNK1 antibody by Western Blot.](https://resources.rndsystems.com/images/datasheets/antibody/JNK1_MAB17761_Western_Blot_5991.jpg)
Detection of Human, Mouse, and Rat JNK1 by Western Blot. Western blot shows lysates of CHP-100 human neuroblastoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for JNK1 was detected at approximately 46 kDa and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
![JNK1 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC). JNK1 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).](https://resources.rndsystems.com/images/datasheets/antibody/JNK1_MAB17761_Immunocytochemistry__Immunofluorescence_20184.jpg)
JNK1 in MCF‑7 Human Cell Line. JNK1 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: JNK1
The c-Jun N-terminal kinases (JNKs) are encoded by three genes: JNK1, JNK2, and JNK3. Members of the MAPK superfamily, JNKs are activated by environmental stresses and inflammatory cytokines. JNK1, also known as SAPK1 gamma and MAPK8, is expressed as four isoforms generated by alternative splicing. JNK1 is activated by dual phosphorylation at T183 and Y185 by the MAPK kinases MKK4 and/or MKK7.
Product Datasheets
Citations for Human/Mouse/Rat JNK1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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APP upregulation contributes to retinal ganglion cell degeneration via JNK3
Authors: C Liu, CW Zhang, Y Zhou, WQ Wong, LC Lee, WY Ong, SO Yoon, W Hong, XY Fu, TW Soong, EH Koo, LW Stanton, KL Lim, ZC Xiao, GS Dawe
Cell Death Differ., 2017-12-13;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Phosphorylation Sites Identified in the NEIL1 DNA Glycosylase Are Potential Targets for the JNK1 Kinase
PLoS ONE, 2016-08-12;11(8):e0157860.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Distinct role of c-Jun N-terminal kinase isoforms in human neutrophil apoptosis regulated by tumor necrosis factor-alpha and granulocyte-macrophage colony-stimulating factor.
Authors: Kato T, Noma H, Kitagawa M, Takahashi T, Oshitani N, Kitagawa S
J. Interferon Cytokine Res., 2008-04-01;28(4):235-43.
Species: Human
Sample Types: Cell Lysates
Applications: ELISA Development
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