Human/Mouse/Rat HSP70/HSPA1A Antibody

Catalog # Availability Size / Price Qty
AF1663
AF1663-SP
Detection of Human, Mouse, and Rat HSP70/HSPA1A by Western Blot.
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Product Details
Citations (14)
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Human/Mouse/Rat HSP70/HSPA1A Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects the induced form of recombinant endogenous human, mouse and rat HSP70/HSPA1A (HSP72) in Western blots. In Western blots, no cross‑reactivity with the constitutively expressed HSC70 (HSP73) is detected.
Source
Polyclonal Rabbit IgG
Purification
Antigen and protein A Affinity-purified
Immunogen
E. coli-derived recombinant human HSP70
Met1-Asp641
Accession # P08107
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat HSP70/HSPA1A antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat HSP70/HSPA1A by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, L-929 mouse fibroblast cell line, and C6 rat glioma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat HSP70/HSPA1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1663) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for HSP70/HSPA1A at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western View Larger

Detection of Human HSP70/HSPA1A by Simple WesternTM. Simple Western shows lysates of Exosome Standards (HT‑29) (NBP3-11685), Exosome Standards (Human Urine) (NBP2-49840) and human liver tissue, loaded at 0.5 mg/ml. A specific band was detected for HSP70/HSPA1A at approximately 67 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human/Mouse/Rat HSP70/HSPA1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1663). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Simple Western Detection of Human HSP70/HSPA1A antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human HSP70/HSPA1A by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with heat shocked (HS), loaded at 0.2 mg/mL. A specific band was detected for HSP70/HSPA1A at approximately 66 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat HSP70/HSPA1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1663). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western Detection of Human and Mouse HSP70/HSPA1A antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human and Mouse HSP70/HSPA1A by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP70/HSPA1A at approximately 66 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat HSP70/HSPA1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1663). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunohistochemistry Detection of Mouse HSP70/HSPA1A by Immunohistochemistry View Larger

Detection of Mouse HSP70/HSPA1A by Immunohistochemistry RFA increases HSP70 levels and activates MyD88. a C57BL/6 mice were exposed to RF and skin HSP70, HSc70, and HSP90 levels were then analyzed by western blotting at 6 and 24 h (hr) using GAPDH as internal control. b Representative IHC images of HSP70 expression in RF-treated and non-treated skin at 24 h. Scale: 250 µm. c WT and MyD88 KO mice were exposed to RF or sham treatment followed by ID injection of 10 µg OVA into RF or sham-treated skin. Serum anti-OVA antibody titer was measured 2 weeks later. n = 4–5. d WT mice were intradermally injected with 100 µg Pepinh-Control or Pepinh-MyD, or the same volume of PBS 3 and 1 h before RF treatment and ID OVA immunization at 10 µg dose. OVA immunization alone served as control (No adjuvant). Serum anti-OVA antibody titer was measured 2 weeks later. n = 4–5. One-way ANOVA with Tukey’s multiple comparison test was used to compare differences between groups in c and d. *p < 0.05; **p < 0.01. NS not significant. Representative of two independent experiments in c and d Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30209303), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse HSP70/HSPA1A by Western Blot View Larger

Detection of Mouse HSP70/HSPA1A by Western Blot RFA increases HSP70 levels and activates MyD88. a C57BL/6 mice were exposed to RF and skin HSP70, HSc70, and HSP90 levels were then analyzed by western blotting at 6 and 24 h (hr) using GAPDH as internal control. b Representative IHC images of HSP70 expression in RF-treated and non-treated skin at 24 h. Scale: 250 µm. c WT and MyD88 KO mice were exposed to RF or sham treatment followed by ID injection of 10 µg OVA into RF or sham-treated skin. Serum anti-OVA antibody titer was measured 2 weeks later. n = 4–5. d WT mice were intradermally injected with 100 µg Pepinh-Control or Pepinh-MyD, or the same volume of PBS 3 and 1 h before RF treatment and ID OVA immunization at 10 µg dose. OVA immunization alone served as control (No adjuvant). Serum anti-OVA antibody titer was measured 2 weeks later. n = 4–5. One-way ANOVA with Tukey’s multiple comparison test was used to compare differences between groups in c and d. *p < 0.05; **p < 0.01. NS not significant. Representative of two independent experiments in c and d Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30209303), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HSP70/HSPA1A

Heat shock proteins (HSPs) are a family of highly conserved stress response proteins. Heat shock proteins function primarily as molecular chaperones by facilitating the folding of other cellular proteins, preventing protein aggregation or targeting improperly folded proteins to specific degradative pathways. HSPs are typically expressed at low levels under normal physiological conditions but are dramatically up‑regulated in response to cellular stress. HSP70 is a 72 kDa member of the heat shock protein 70 family of proteins.  HSP70, also known as HSPA1A, HSP70-1, and HSP72 is a 641 amino acid (aa) heat shock protein. Over aa 1-641, human HSP70 shares 95% and 97% aa identity to mouse and rat HSP70.

Long Name
Heat Shock Protein 70
Entrez Gene IDs
3303 (Human); 15511 (Mouse); 24472 (Rat)
Alternate Names
dnaK-type molecular chaperone HSP70-1; FLJ54303; FLJ54370; FLJ54392; FLJ54408; FLJ75127; Heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A/1B; heat shock 70kD protein 1A; heat shock 70kDa protein 1A; heat shock-induced protein; HSP70; HSP70.1/HSP70.2; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70I; HSP72; HSPA1; HSPA1A; HSPA1B

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Citations for Human/Mouse/Rat HSP70/HSPA1A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. FOX transcription factors are common regulators of Wnt/ beta -catenin–dependent gene transcription
    Authors: Lavanya Moparthi, Stefan Koch
    Journal of Biological Chemistry
  2. The tumour suppressor p53 is a negative regulator of the carcinoma-associated transcription factor FOXQ1
    Authors: Pizzolato, G;Moparthi, L;Pagella, P;Cantù, C;D'Arcy, P;Koch, S;
    The Journal of biological chemistry
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Divergent single cell transcriptome and epigenome alterations in ALS and FTD patients with C9orf72 mutation
    Authors: Li J, Jaiswal MK, Chien JF et al.
    Nat Commun
    Species: Human
    Sample Types:
    Applications: Simple Western
  4. The oncogenic transcription factor FOXQ1 is a differential regulator of Wnt target genes
    Authors: G Pizzolato, L Moparthi, S Söderholm, C Cantù, S Koch
    Journal of Cell Science, 2022-10-10;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Plasma extracellular vesicles bearing PD-L1, CD40, CD40L or TNF-RII are significantly reduced after treatment of AIDS-NHL
    Authors: LE Martínez, S Lensing, D Chang, LI Magpantay, R Mitsuyasu, RF Ambinder, JA Sparano, O Martínez-M, M Epeldegui
    Oncogene, 2022-06-02;12(1):9185.
    Species: Human
    Sample Types: Extracellular Vesicle Lysate
    Applications: Western Blot
  6. Acute Ethanol Exposure during Synaptogenesis Rapidly Alters Medium Spiny Neuron Morphology and Synaptic Protein Expression in the Dorsal Striatum
    Authors: E Clabough, J Ingersoll, T Reekes, A Gleichsner, A Ryan
    International Journal of Molecular Sciences, 2021-12-28;23(1):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Long-Term Low-Dose Delta-9-Tetrahydrocannbinol (THC) Administration to Simian Immunodeficiency Virus (SIV) Infected Rhesus Macaques Stimulates the Release of Bioactive Blood Extracellular Vesicles (EVs) that Induce Divergent Structural Adaptations and Signaling Cues
    Authors: Y Lyu, S Kopcho, M Mohan, CM Okeoma
    Cells, 2020-10-06;9(10):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. The APMAP interactome reveals new modulators of APP processing and beta-amyloid production that are altered in Alzheimer's disease
    Authors: H Gerber, S Mosser, B Boury-Jamo, M Stumpe, A Piersigill, C Goepfert, J Dengjel, U Albrecht, F Magara, PC Fraering
    Acta Neuropathol Commun, 2019-01-31;7(1):13.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. Augmentation of vaccine-induced humoral and cellular immunity by a physical radiofrequency adjuvant
    Authors: Y Cao, X Zhu, MN Hossen, P Kakar, Y Zhao, X Chen
    Nat Commun, 2018-09-12;9(1):3695.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, IHC-P, Western Blot
  10. A role for heat shock factor 1 in hypercapnia-induced inhibition of inflammatory cytokine expression
    Authors: Z Lu, SM Casalino-M, A Nair, A Buchbinder, GRS Budinger, PHS Sporn, KL Gates
    FASEB J., 2018-02-05;0(0):fj201701164R.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Substance P acts via the neurokinin receptor 1 to elicit bronchoconstriction, oxidative stress, and upregulated ICAM-1 expression after oil smoke exposure.
    Authors: Li PC, Chen WC, Chang LC, Lin SC
    Am. J. Physiol. Lung Cell Mol. Physiol., 2008-03-07;294(5):L912-20.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  12. Precipitation with polyethylene glycol followed by washing and pelleting by ultracentrifugation enriches extracellular vesicles from tissue culture supernatants in small and large scales
    Authors: Anna-Kristin Ludwig, Kyra De Miroschedji, Thorsten R. Doeppner, Verena Börger, Johannes Ruesing, Vera Rebmann et al.
    Journal of Extracellular Vesicles
  13. CRN2 binds to TIMP4 and MMP14 and promotes perivascular invasion of glioblastoma cells
    Authors: Solga R, Behrens J, Ziemann A et al.
    European Journal of Cell Biology
  14. RNA-binding protein ZFP36L1 regulates osteoarthritis by modulating members of the heat shock protein 70 family
    Authors: YO Son, HE Kim, WS Choi, CH Chun, JS Chun
    Nat Commun, 2019-01-08;10(1):77.

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