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Human/Mouse/Rat HSP60 Antibody

Catalog #: AF1800
5 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1800
AF1800-SP
Detection of Human/Mouse/Rat HSP60 by Western Blot.
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Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat HSP60 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat HSP60 in Western blots.
Source
Polyclonal Rabbit IgG
Purification
Antigen and protein A Affinity-purified
Immunogen
E. coli-derived recombinant human HSP60
Met1-Phe573
Accession # P10809
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
0.5 - 1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat HSP60 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat HSP60 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF-7 human breast cancer cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat HSP60 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1800) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for HSP60 at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human and Mouse HSP60 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human and Mouse HSP60 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP60 at approximately 60 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat HSP60 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1800). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western View Larger

Detection of Human, Mouse, and Rat HSP60 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and C6 rat glioma cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP60 at approximately 59 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human/Mouse/Rat HSP60 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1800). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HSP60

Heat shock proteins (HSPs) are a family of highly conserved stress response proteins. Heat shock proteins function primarily as molecular chaperones by facilitating the folding of other cellular proteins, preventing protein aggregation or targeting improperly folded proteins to specific degradative pathways. HSPs are typically expressed at low levels under normal physiological conditions but are dramatically up-regulated in response to cellular stress. Heat Shock Protein 60 (HSP60), also known as Chaperonin 60 (CPN60), is a mitochondrial matrix protein belonging to a highly conserved family of molecular chaperone and stress response proteins. HSP60 plays a role in stabilizing and refolding proteins in response to heat-shock or other cellular stress.  Full length human HSP60 is 98% identical to mouse and rat HSP60.

Long Name
Heat Shock Protein 60
Entrez Gene IDs
3329 (Human); 110907 (Mouse); 63868 (Rat)
Alternate Names
60 kDa chaperonin; Chaperonin 60; cpn60; GROEL; heat shock 60kD protein 1 (chaperonin); heat shock 60kDa protein 1 (chaperonin); Heat shock protein 60; heat shock protein 65; HLD4; HSP60; HSP-60; HSP60SPG13; HSP65; HSPD1; HuCHA60; Mitochondrial matrix protein P1,60 kDa heat shock protein, mitochondrial; P60 lymphocyte protein; short heat shock protein 60 Hsp60s1; spastic paraplegia 13 (autosomal dominant); SPG13

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Citations for Human/Mouse/Rat HSP60 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Increased Systemic Antioxidant Power Ameliorates the Aging-Related Reduction in Oocyte Competence in Mice
    Authors: Lane SL, Parks JC, Russ JE et al.
    International Journal of Molecular Sciences
  2. Cytochrome P450 3A4 (CYP3A4) protein quantification using capillary western blot technology and total protein normalization
    Authors: JM Collins, D Wang
    Journal of pharmacological and toxicological methods, 2021-08-30;112(0):107117.
    Species: Human
    Sample Types: Tissue Lysates
    Applications: Simple Western
  3. Differential Subcellular Distribution and Translocation of Seven 14-3-3 Isoforms in Response to EGF and During the Cell Cycle
    Authors: A Abdrabou, D Brandwein, Z Wang
    Int J Mol Sci, 2020-01-02;21(1):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Protein-based identification of quantitative trait loci associated with malignant transformation in two HER2+ cellular models of breast cancer
    Authors: Yogesh M Kulkarni, David J Klinke
    Proteome Science
  5. Increased Systemic Antioxidant Power Ameliorates the Aging-Related Reduction in Oocyte Competence in Mice
    Authors: Lane SL, Parks JC, Russ JE et al.
    International Journal of Molecular Sciences

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Reviews for Human/Mouse/Rat HSP60 Antibody

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Human/Mouse/Rat HSP60 Antibody
By Khuynh Nguyen on 04/18/2022
Application: WB Sample Tested: MDA-MB-231 human breast cancer cell line Species: Human
Western Blot Human/Mouse/Rat HSP60 Antibody AF1800