Human/Mouse/Rat HO-2/HMOX2 Antibody

Catalog # Availability Size / Price Qty
AF3170
AF3170-SP
Detection of Human/Mouse/Rat HO‑2/HMOX2 by Western Blot.
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Human/Mouse/Rat HO-2/HMOX2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
The antibody is known to react with endogenous human, mouse, and rat HO-2 in in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human HO-2/HMOX2
Met1-Met316
Accession # P30519
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat HO‑2/HMOX2 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat HO‑2/HMOX2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, DA3 mouse myeloma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat HO-2/HMOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3170) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HO-2/HMOX2 at approximately 38 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Simple Western Detection of Human, Mouse, and Rat HO-2/HMOX2 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human, Mouse, and Rat HO‑2/HMOX2 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, PC-12 rat adrenal pheochromocytoma cell line, DA3 mouse myeloma cell line, and C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for HO-2/HMOX2 at approximately 43 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat HO-2/HMOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3170) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western Detection of Mouse HO-2/HMOX2 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse HO‑2/HMOX2 by Simple WesternTM. Simple Western lane view shows lysates of DA3 mouse myeloma cell line and C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for HO-2/HMOX2 at approximately 43 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat HO-2/HMOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3170) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HO-2/HMOX2

Heme Oxygenase 2 (HO-2), also known as HMOX2, is a 36 kDa microsomal enzyme required for the metabolism of heme to biliverdin. Heme oxygenase occurs as 2 isozymes, the constitutively expressed heme oxygenase-2 (HO-2/HMOX2) and the inducible heme oxygenase-1 (HO-1/HMOX1). HO-1 expression is induced by heme and other non-heme compounds. Human HO-2 shares 42% amino acid sequence identity with human HO-1 and 89% amino acid sequence identity with mouse and rat HO-2.

Long Name
Heme Oxygenase 2
Entrez Gene IDs
3163 (Human); 15369 (Mouse); 79239 (Rat)
Alternate Names
heme oxygenase (decycling) 2; heme oxygenase 2; HMOX2; HO2; HO-2; HO-2EC 1.14.99.3

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