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Human/Mouse/Rat Fyn Antibody

Catalog #: MAB3574
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3574
MAB3574-SP
Detection of Human/Mouse/Rat Fyn by Western Blot.
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Product Details
Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Fyn Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects endogenous human, mouse and rat Fyn in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 361228
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Fyn
Met1-Gly83
Accession # P06241
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat Fyn antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat Fyn by Western Blot. Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line, C2C12 mouse myoblast cell line, CTLL-2 mouse cytotoxic T cell line, NIH-3T3 mouse embryonic fibroblast cell line, PT18 mouse mast/basophil cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Fyn Monoclonal Antibody (Catalog # MAB3574) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Fyn at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunocytochemistry Fyn antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

Fyn in Human PBMCs. Fyn was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human/Mouse/Rat Fyn Monoclonal Antibody (Catalog # MAB3574) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fyn

Fyn is a ubiquitously expressed member of the Src family of intracellular tyrosine kinases. Fyn activity is implicated in T cell receptor signaling, adhesion-mediated signaling, and regulation of brain function. Mice lacking fyn display various deficits, including impaired spatial memory, hypomyelination, and uncoordinated hippocampal structure, suggesting that Fyn is involved in multiple neural signaling pathways.

Long Name
FYN oncogene related to SRC, FGR, YES
Entrez Gene IDs
2534 (Human)
Alternate Names
EC 2.7.10; EC 2.7.10.2; FYN oncogene related to SRC, FGR, YES; Fyn; MGC45350; OKT3-induced calcium influx regulator; p59-Fyn; protein-tyrosine kinase fyn; Proto-oncogene c-Fyn; Proto-oncogene Syn; proto-oncogene tyrosine-protein kinase fyn; SLK; SLKc-syn protooncogene; src/yes-related novel; Src-like kinase; SYN; tyrosine kinase p59fyn(T); tyrosine-protein kinase Fyn

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Citations for Human/Mouse/Rat Fyn Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Docking of Syk to Fc epsilon RI is enhanced by Lyn but limited in duration by SHIP1
    Authors: William K. Kanagy, Cédric Cleyrat, Mohamadreza Fazel, Shayna R. Lucero, Marcel P. Bruchez, Keith A. Lidke et al.
    Molecular Biology of the Cell
  2. Calcium-dependent ESCRT recruitment and lysosome exocytosis maintain epithelial integrity during Candida albicans invasion
    Authors: Westman J, Plumb J, Licht A et al.
    Cell reports
  3. An activity-dependent determinant of synapse elimination in the mammalian brain
    Authors: Masahiro Yasuda, Sivapratha Nagappan-Chettiar, Erin M. Johnson-Venkatesh, Hisashi Umemori
    Neuron

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