Human/Mouse/Rat eIF4E Antibody

Name: Human/Mouse/Rat eIF4E Antibody
Brand: R&D Systems
SKU: MAB3228
Rating: 5 (1 reviews)

Catalog #: MAB3228 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3228
MAB3228-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat eIF4E Antibody Summary

Specificity

Detects human, mouse and rat eIF4E.

Source

Monoclonal Mouse IgG_2B Clone # 299910

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human eIF4E
Met1-Val217
Accession # P06730

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Knockout Validated

eIF4E is specifically detected in MCF‑7 human breast cancer cell line parental cell line but is not detectable in eIF4E knockout MCF‑7 cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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eIF4E in MCF-7 Human Cell Line. eIF4E was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Mouse Anti-Human/Mouse/Rat eIF4E Monoclonal Antibody (Catalog # MAB3228) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western Blot

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Detection of Human/Mouse/Rat eIF4E by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat eIF4E Monoclonal Antibody (Catalog # MAB3228) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for eIF4E at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Knockout Validated

Western Blot Shows Human eIF4E Antibody Specificity by Using Knockout Cell Line.

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Western Blot Shows Human eIF4E Specificity by Using Knockout Cell Line. Western blot shows lysates of MCF-7 human breast cancer parental cell line and eIF4E knockout MCF-7 cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat eIF4E Monoclonal Antibody (Catalog # MAB3228) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for eIF4E at approximately 25 kDa (as indicated) in the parental MCF-7 cell line, but is not detectable in knockout MCF-7 cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: eIF4E

eIF4E (eukaryotic initiation factor 4E) interacts with the 7-methyl-GTP cap structure to facilitate the initiation and rate of translation of mRNA. Together with eIF4G and eIF4A, it forms the eIF4F complex. eIF4E activity has been shown to play a role in cell cycle progression, tumorigenesis, embryonic development, nuclear export and synaptic plasticity.

Long Name

Eukaryotic Translation Initiation Factor 4E

Entrez Gene IDs

1977 (Human); 13684 (Mouse); 117045 (Rat)

Alternate Names

CBP; eIF4E; eIF-4E; EIF4E1; EIF4EL1; EIF4EL1MGC111573; eIF-4F 25 kDa subunit; EIF4F; eukaryotic translation initiation factor 4E; eukaryotic translation initiation factor 4E-like 1; mRNA cap-binding protein