Human/Mouse/Rat DRAK2 Antibody

Catalog #: MAB6640 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6640
MAB6640-SP

Detection of Human, Mouse, and Rat DRAK2 by Western Blot.

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Human/Mouse/Rat DRAK2 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat DRAK2 in Western blots. In Western blots, less than 10% cross-reactivity with recombinant human (rh) DRAK1, rhDAPK1, rhDAPK2, or rhDAPK3 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 685228

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human DRAK2
Ala87-Glu285 (predicted)
Accession # O94768

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat DRAK2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF Membrane was probed with 1 µg/mL of Human DRAK2 Monoclonal Antibody (Catalog # MAB6640) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DRAK2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DRAK2

DRAK2 (death-associated protein related kinase-2), also called STK17B (Ser/Thr kinase 17B) is a 45 kDa member of the DAPK (death-associated protein kinase) family. It is expressed mainly in lymphoid organs by mature T cells and B cells. It is autophosphorylated on Ser12 after TCR stimulation and negatively regulates TCR signaling. Ultraviolet light causes phosphorylation on Ser350 and translocation to the nucleus to stimulate apoptosis. Within the region used as an immunogen, human DRAK2 shares 95% amino acid (aa) identity with mouse and rat DRAK2 while mouse and rat DRAK2 share 99.5% aa identity.

Long Name

DAP Kinase-related Apoptosis-inducing Protein Kinase 2

Entrez Gene IDs

9262 (Human)

Alternate Names

DAP kinase-related apoptosis-inducing protein kinase 2; DRAK2; DRAK2death-associated protein kinase-related 2; EC 2.7.11; EC 2.7.11.1; serine/threonine kinase 17b (apoptosis-inducing); serine/threonine kinase 17b; serine/threonine-protein kinase 17B; STK17B

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