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Human/Mouse/Rat/Chicken Oligodendrocyte Marker O4 PE‑conjugated Antibody

Catalog #: FAB1326P
13 Citations Datasheet / COA / SDS
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FAB1326P
Detection of Oligodendrocyte Marker O4 in Rat Cortical Stem Cells by Flow Cytometry.
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Citations (13)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat/Chicken Oligodendrocyte Marker O4 PE‑conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat, Chicken
Specificity
Detects human, mouse, rat, and chicken Oligodendrocyte Marker O4.
Source
Monoclonal Mouse IgM Clone # O4
Purification
IgM-specific Affinity-purified from hybridoma culture supernatant
Immunogen
Bovine brain corpus callosum white matter
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Oligodendrocyte Marker O4 antibody in Rat Cortical Stem Cells antibody by Flow Cytometry. View Larger

Detection of Oligodendrocyte Marker O4 in Rat Cortical Stem Cells by Flow Cytometry. Rat cortical stem cells either differentiated (filled histogram) or undifferentiated (open histogram) were stained with Mouse Anti-Human/Mouse/Rat/ Chicken Oligodendrocyte Marker O4 PE‑conjugated Monoclonal Antibody (Catalog # FAB1326P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Oligodendrocyte Marker O4

Oligodendrocytes are myelinating cells in the central nervous system (CNS) and form the myelin sheath of axons to support rapid nerve conduction. Oligodendrocyte Marker O4 is an antigen on the surface of oligodendrocyte progenitors (1, 2). It has been commonly used as the earliest recognized marker specific for the oligodendroglial lineage (3-8).

References
  1. Schachner, M. et al. (1981) Dev. Biol. 83:328.
  2. Bansal, R. et al. (1989) J. Neurosci. Res. 24:548.
  3. Bansal, R. and Pfeiffer, S.E. (1989) Proc. Natl. Acad. Sci. USA 86:6181.
  4. Gard, A. et al. (1995) Dev. Biol. 167:596.
  5. Reynolds, R. and Hardy, R. (1997) J. Neurosci. Res. 47:455.
  6. Ono, K. et al. (1997) J. Neurosci. Res. 48:212.
  7. Pang, Y. et al. (2000) J. Neurosci. Res. 62:510.
  8. Cai, Z. et al. (2001) Brain Res. 898:126.
Alternate Names
Oligodendrocyte Marker O4

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Citations for Human/Mouse/Rat/Chicken Oligodendrocyte Marker O4 PE‑conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Transplantable human motor networks as a neuron-directed strategy for spinal cord injury
    Authors: Zachary T. Olmsted, Cinzia Stigliano, Annalisa Scimemi, Tatiana Wolfe, Jose Cibelli, Philip J. Horner et al.
    iScience
  2. Induction of early neural precursors and derivation of tripotent neural stem cells from human pluripotent stem cells under xeno-free conditions
    Authors: Hal X. Nguyen, Usha Nekanti, Daniel L. Haus, Gabrielle Funes, Denisse Moreno, Noriko Kamei et al.
    Journal of Comparative Neurology
  3. Isolation of rat Schwann cells based on cell sorting
    Authors: Mi Shen, Wei Tang, Zheng Cao, Xuemin Cao, Fei Ding
    Molecular Medicine Reports
  4. Astrocytic lactate dehydrogenase A regulates neuronal excitability and depressive-like behaviors through lactate homeostasis in mice
    Authors: S Yao, MD Xu, Y Wang, ST Zhao, J Wang, GF Chen, WB Chen, J Liu, GB Huang, WJ Sun, YY Zhang, HL Hou, L Li, XD Sun
    Nature Communications, 2023-02-09;14(1):729.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Identification of astrocyte regulators by nucleic acid cytometry
    Authors: IC Clark, MA Wheeler, HG Lee, Z Li, LM Sanmarco, S Thaploo, CM Polonio, SW Shin, G Scalisi, AR Henry, JM Rone, F Giovannoni, M Charabati, CF Akl, DM Aleman, SEJ Zandee, A Prat, DC Douek, EA Boritz, FJ Quintana, AR Abate
    Nature, 2023-01-04;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Deletion of the Sodium-Dependent Glutamate Transporter GLT-1 in Maturing Oligodendrocytes Attenuates Myelination of Callosal Axons During a Postnatal Phase of Central Nervous System Development
    Authors: EJ Thomason, E Suárez-Poz, FS Afshari, PA Rosenberg, JL Dupree, B Fuss
    Oncogene, 2022-06-03;16(0):905299.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Gut-licensed IFN&gamma+ NK cells drive LAMP1+TRAIL+ anti-inflammatory astrocytes
    Authors: LM Sanmarco, MA Wheeler, C Gutiérrez-, CM Polonio, M Linnerbaue, FA Pinho-Ribe, Z Li, F Giovannoni, KV Batterman, G Scalisi, SEJ Zandee, ES Heck, M Alsuwailm, DL Rosene, B Becher, IM Chiu, A Prat, FJ Quintana
    Nature, 2021-01-06;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. MiRNA-210 induces microglial activation and regulates microglia-mediated neuroinflammation in neonatal hypoxic-ischemic encephalopathy
    Authors: B Li, C Dasgupta, L Huang, X Meng, L Zhang
    Cell. Mol. Immunol., 2019-07-12;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Microenvironment in neuroblastoma: isolation and characterization of tumor-derived mesenchymal stromal cells
    Authors: G Pelizzo, V Veschi, M Mantelli, S Croce, V Di Benedet, P D'Angelo, A Maltese, L Catenacci, T Apuzzo, E Scavo, A Moretta, M Todaro, G Stassi, MA Avanzini, V Calcaterra
    BMC Cancer, 2018-11-27;18(1):1176.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Oligodendrocyte precursor cell transplantation into organotypic cerebellar shiverer slices: a model to study myelination and myelin maintenance.
    Authors: Bin JM, Leong SY, Bull SJ
    PLoS ONE, 2012-07-20;7(7):e41237.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Toll-like receptor 3 regulates neural stem cell proliferation by modulating the Sonic Hedgehog pathway.
    Authors: Yaddanapudi K, De Miranda J, Hornig M
    PLoS ONE, 2011-10-25;6(10):e26766.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Metabolic Control of Astrocyte Pathogenic Activity via cPLA2-MAVS
    Authors: Chun-Cheih Chao, Cristina Gutiérrez-Vázquez, Veit Rothhammer, Lior Mayo, Michael A. Wheeler, Emily C. Tjon et al.
    Cell
  13. Environmental Control of Astrocyte Pathogenic Activities in CNS Inflammation
    Authors: Wheeler MA, Jaronen M, Covacu R et al.
    Cell

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