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Human/Mouse/Rat/Chicken A2B5 Biotinylated Antibody

Catalog #: BAM1416
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BAM1416
Detection of A2B5 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Human/Mouse/Rat/Chicken A2B5 Biotinylated Antibody Summary

Species Reactivity
Human, Mouse, Rat, Chicken
Specificity
Recognizes the A2B5 cell surface ganglioside epitope found on type II astrocytes, cells involved in gliogenesis, and cells committed to the oligodendrocyte lineage (1‑4).
Source
Monoclonal Mouse IgM Clone # 105
Purification
IgM-specific Affinity-purified from hybridoma culture supernatant
Immunogen
Embryonic chicken retinal cells
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of A2B5 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of A2B5 in RAW 264.7 Mouse Cell Line by Flow Cytometry. NTera-2 human testicular embryonic carcinoma cell line either treated with 10 µM retinoic acid for 4 days (filled histogram) or untreated (open histogram) was stained with Mouse Anti-Human/Mouse/Rat/Chicken A2B5 Biotinylated Monoclonal Antibody (Catalog # BAM1416), followed by Streptavidin-Allophycocyanin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: A2B5

A2B5 is a cell surface ganglioside epitope expressed in developing thymic epithelial cells, oligodendrocyte progenitors, and neuroendocrine cells (1).

References
  1. Eisenbarth, G.S. et al. (1979) Proc. Natl. Acad. Sci. USA 76:4913.
  2. Bottenstein, J.E. et al. (1988) J. Neurosci. Res. 20:291.
  3. Suzumura, A. and D.H. Silberberg (1989) Brain Res. 480:51.
  4. Dubois-Dalcq, M. et al. (1990) Cellular and Molecular Biology of Myelination, Springer-Verlag, pp. 3.
Alternate Names
A2B5 marker; A2B5

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Citation for Human/Mouse/Rat/Chicken A2B5 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Fluorescent protein-expressing neural progenitor cells as a tool for transplantation studies.
    Authors: Skardelly M, Hempel E, Hirrlinger J, Wegner F, Meixensberger J, Milosevic J
    PLoS ONE, 2014-06-16;9(6):e99819.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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