Human/Mouse/Rat Caveolin-1 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC5736G
Caveolin‑1 in HeLa Human Cell Line.
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Citations (1)
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Human/Mouse/Rat Caveolin-1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Designed to visualize the expression of Caveolin-1 by fluorescence microscopy for staining cells and tissues. Conjugated Caveolin-1 antibodies are ideal for immunocytochemistry colocalization studies in caveolae. Detects endogenous human, mouse and rat Caveolin-1 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 7C8
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Puified rat adipocyte low density microsomes
Accession # P41350
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Immunocytochemistry
1:10 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Caveolin-1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

Caveolin‑1 in HeLa Human Cell Line. Caveolin‑1 was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Caveolin‑1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC5736G) at 1:10 dilution overnight at 4° C and counterstained with Propidium Iodide (red). Specific staining was localized to caveolae. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Caveolin-1

Caveolin-1 is a palmitoylated 22 kDa membrane-associated protein in caveolae, the cholesterol-rich invaginations in the plasma membrane involved in vesicular transport and regulation of lipid rafts. Caveolin-1 expression is dysregulated during cancer progression and exhibits both positive and negative effects on tumor progression. The central region of Caveolin-1 (aa 105‑125) is buried in the lipid layer, while the N- and C-terminal flanking regions are exposed to the cytoplasm and interact with many other proteins. Within these cytoplasmic regions, human Caveolin-1 shares 95% aa sequence identity with mouse and rat Caveolin-1. Alternate splicing in human, mouse and rat generates an isoform with a deletion of the N-terminal 31 residues.

Entrez Gene IDs
857 (Human); 12389 (Mouse); 25404 (Rat)
Alternate Names
CAV; CAV1; caveolae protein, 22kD; caveolin 1, caveolae protein, 22kDa; Caveolin1; Caveolin-1; cell growth-inhibiting protein 32; MSTP085; VIP21

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citation for Human/Mouse/Rat Caveolin-1 Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Faster, sharper, more precise: Automated Cluster-FLIM in preclinical testing directly identifies the intracellular fate of theranostics in live cells and tissue
    Authors: R Brodwolf, P Volz-Rakeb, J Stellmache, C Wolff, M Unbehauen, R Haag, M Schäfer-Ko, C Zoschke, U Alexiev
    Theranostics, 2020-05-15;10(14):6322-6336.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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