Human/Mouse/Rat Carbonic Anhydrase III/CA3 Antibody
Human/Mouse/Rat Carbonic Anhydrase III/CA3 Antibody Summary
Ala2-Lys260
Accession # P07451
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat Carbonic Anhydrase III/CA3 by Western Blot. Western blot shows lysates of human, mouse, and rat skeletal muscle tissue and heart tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse/ Rat Carbonic Anhydrase III/CA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2185) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Carbonic Anhydrase III/CA3 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carbonic Anhydrase III/CA3
Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1).
Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA3 is a cytosolic enzyme with a very low CA activity. It is expressed at low levels in human muscle during early development but increases rapidly during the last trimester to reach 50‑60% of adult levels at birth (2).
- Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
- Sly, W.S. and P.Y. Hu (1995) Annu. Rev. Biochem. 64:375.
Product Datasheets
Citations for Human/Mouse/Rat Carbonic Anhydrase III/CA3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Effect of resistance training and protein intake pattern on myofibrillar protein synthesis and proteome kinetics in older men in energy restriction
Authors: CH Murphy, M Shankaran, TA Churchward, CJ Mitchell, NM Kolar, LM Burke, JA Hawley, A Kassis, LG Karagounis, K Li, C King, M Hellerstei, SM Phillips
J. Physiol. (Lond.), 2018-04-17;0(0):.
Species: Human
Sample Types: Serum
Applications: Immunoprecipitation -
Comprehensive proteomics analysis of autophagy-deficient mouse liver.
Authors: Matsumoto N, Ezaki J, Komatsu M, Takahashi K, Mineki R, Taka H, Kikkawa M, Fujimura T, Takeda-Ezaki M, Ueno T, Tanaka K, Kominami E
Biochem. Biophys. Res. Commun., 2008-02-04;368(3):643-9.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Circulating protein synthesis rates reveal skeletal muscle proteome dynamics
Authors: Mahalakshmi Shankaran, Chelsea L. King, Thomas E. Angel, William E. Holmes, Kelvin W. Li, Marc Colangelo et al.
Journal of Clinical Investigation
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