Home / AMPK alpha 2 / Human/Mouse/Rat AMPK alpha 2 Antibody

Human/Mouse/Rat AMPK alpha 2 Antibody

Catalog #: AF2850
11 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2850
AF2850-SP
Detection of Human and Mouse AMPK alpha 2 by Western Blot.
3 Images
Product Details
Citations (11)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat AMPK alpha 2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat AMPK alpha 2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human AMPK alpha 1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human AMPK alpha 2
Phe340-Arg552
Accession # P54646
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse liver
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse AMPKa2 antibody by Western Blot. View Larger

Detection of Human and Mouse AMPK alpha 2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat AMPKa2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2850) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for AMPKa2 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human AMPKa2 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human AMPK alpha 2 by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for AMPKa2 at approximately 63 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat AMPKa2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2850) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunocytochemistry AMPKa2 antibody in HEK293 Human Cell Line by Immunocytochemistry (ICC). View Larger

AMPK alpha 2 in HEK293 Human Cell Line. AMPKa2 was detected in immersion fixed HEK293 human embryonic kidney cell line using Goat Anti-Human/Mouse/Rat AMPKa2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2850) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AMPK alpha 2

AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic alpha subunit and regulatory beta and gamma subunits. Each subunit exists as alternate isoforms ( alpha 1, alpha 2, beta 1, beta 2, gamma 1, gamma 2, gamma 3), with all 12 combinations able to form complexes. The catalytic alpha subunit of AMPK is activated allosterically by AMP, and by phosphorylation via the AMPK kinase LKB1. AMPK’s role in metabolic regulation has implicated this serine/threonine kinase as a therapeutic target in heart disease, obesity, and diabetes.

Long Name
AMP-activated Protein Kinase alpha
Entrez Gene IDs
5563 (Human); 108079 (Mouse); 78975 (Rat)
Alternate Names
5'-AMP-activated protein kinase catalytic subunit alpha-2; AMPK alpha 2; AMPK subunit alpha-2; AMPK2; AMPK5'-AMP-activated protein kinase, catalytic alpha-2 chain; AMPK-alpha-2 chain; EC 2.7.11; EC 2.7.11.1; PRKAA; PRKAA2; protein kinase, AMP-activated, alpha 2 catalytic subunit

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human/Mouse/Rat AMPK alpha 2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Loss of the E3 ubiquitin ligase MKRN1 represses diet-induced metabolic syndrome through AMPK activation
    Authors: MS Lee, HJ Han, SY Han, IY Kim, S Chae, CS Lee, SE Kim, SG Yoon, JW Park, JH Kim, S Shin, M Jeong, A Ko, HY Lee, KJ Oh, YH Lee, KH Bae, SH Koo, JW Kim, JK Seong, D Hwang, J Song
    Nat Commun, 2018-08-24;9(1):3404.
  2. Metformin Promotes Antitumor Immunity via Endoplasmic-Reticulum-Associated Degradation of PD-L1
    Authors: Cha JH, Yang WH, Xia W et al.
    Mol. Cell
  3. MST3 is involved in ENaC-mediated hypertension
    Authors: Te-Jung Lu, Wei-Chih Kan, Sung-Sen Yang, Si-Tse Jiang, Sheng-Nan Wu, Pin Ling et al.
    American Journal of Physiology-Renal Physiology
  4. Expression of 5′-AMP-activated Protein Kinase with Starvation in Murine Thymocytes
    Authors: Rintarou Okoshi, Kazumasa Ohta, Ayako Ishikawa, Yutaka Sato, Harutoshi Kizaki
    The Bulletin of Tokyo Dental College
  5. Catalytic isoforms of AMP-activated protein kinase differentially regulate IMPDH activity and photoreceptor neuron function
    Authors: Lee, TJ;Sasaki, Y;Ruzycki, PA;Ban, N;Lin, JB;Wu, HT;Santeford, A;Apte, RS;
    JCI insight
    Species: Transgenic Mouse
    Sample Types: Cell Lysates, Protein, Whole Tissue
    Applications: Immunohistochemistry, Western Blot
  6. MST3 Involvement in Na+ and K+ Homeostasis with Increasing Dietary Potassium Intake
    Authors: CH Chan, SN Wu, BY Bao, HW Li, TL Lu
    International Journal of Molecular Sciences, 2021-01-20;22(3):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Neuronal AMP-activated protein kinase hyper-activation induces synaptic loss by an autophagy-mediated process
    Authors: M Domise, F Sauvé, S Didier, R Caillerez, S Bégard, S Carrier, M Colin, C Marinangel, L Buée, V Vingtdeux
    Cell Death Dis, 2019-03-04;10(3):221.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. AMP-activated protein kinase modulates tau phosphorylation and tau pathology in vivo
    Authors: Manon Domise
    Sci Rep, 2016-05-27;6(0):26758.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  9. Mitochondria and AMP-activated protein kinase-dependent mechanism of efferocytosis.
    Authors: Jiang S, Park D, Stigler W, Creighton J, Ravi S, Darley-Usmar V, Zmijewski J
    J Biol Chem, 2013-07-29;288(36):26013-26.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Regulation of AMP-activated protein kinase signaling by AFF4 protein, member of AF4 (ALL1-fused gene from chromosome 4) family of transcription factors, in hypothalamic neurons.
    Authors: Komori T, Doi A, Nosaka T, Furuta H, Akamizu T, Kitamura T, Senba E, Morikawa Y
    J. Biol. Chem., 2012-04-23;287(24):19985-96.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  11. The nuclear receptor PPARbeta/delta programs muscle glucose metabolism in cooperation with AMPK and MEF2.
    Authors: Gan Z, Burkart-Hartman EM, Han DH, Finck B, Leone TC, Smith EY, Ayala JE, Holloszy J, Kelly DP
    Genes Dev., 2011-12-01;25(24):2619-30.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human/Mouse/Rat AMPK alpha 2 Antibody

Average Rating: 3 (Based on 1 Review)

5 Star
0%
4 Star
0%
3 Star
100%
2 Star
0%
1 Star
0%

Have you used Human/Mouse/Rat AMPK alpha 2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human/Mouse/Rat AMPK alpha 2 Antibody
By Anonymous on 07/19/2019
Application: WB Sample Tested: Adipose tissue Species: Mouse
Western Blot Human/Mouse/Rat AMPK alpha 2 Antibody AF2850