Human/Mouse/Rat alpha Actinin 2 Antibody
Human/Mouse/Rat alpha Actinin 2 Antibody Summary
Accession # P35609
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat alpha Actinin 2 by Western Blot. Western blot shows lysates of human skeletal muscle tissue, mouse skeletal muscle tissue, mouse heart tissue, and rat skeletal muslce tissue. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human/Mouse/Rat alpha Actinin 2 Monoclonal Antibody (Catalog # MAB9830) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for alpha Actinin 2 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Alpha Actinin 2 in C2C12 mouse myoblast cell line. Alpha Actinin 2 was detected in immersion fixed C2C12 mouse myoblast cell line using Rabbit Anti-Human/Mouse/Rat alpha Actinin 2 Monoclonal Antibody (Catalog # MAB9830) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of alpha Actinin 2 in Human Heart. Alpha Actinin 2 was detected in immersion fixed paraffin-embedded sections of human heart using Rabbit Anti-Human/Mouse/Rat alpha Actinin 2 Monoclonal Antibody (Catalog # MAB9830) at 10 µg/ml for 1 hour at room temperature followed by incubation with the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008) or the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of alpha Actinin 2 in Human Skeletal Muscle. Alpha Actinin 2 was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Rabbit Anti-Human/Mouse/Rat alpha Actinin 2 Monoclonal Antibody (Catalog # MAB9830) at 10 µg/ml for 1 hour at room temperature followed by incubation with the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008) or the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha Actinin 2
Actinins are F-actin cross-linking proteins thought to anchor actin to a variety of intracellular structures. alpha-Actinin 2 (ACTN2) is a member of the spectrin superfamily with a predicted molecular weight of approximately 104 kDa. It is 894 amino acids (aa) in length and shares 99% aa identity with mouse and rat ACTN2. Two splice forms exist, resulting in 2 distinct isoforms. ACTN2 homodimerizes in an anti-parallel fashion, and can also form heterodimers with ACTN3.
Product Datasheets
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