Home / Oct-3/4 / Human/Mouse Oct-3/4 Fluorescein-conjugated Antibody

Human/Mouse Oct-3/4 Fluorescein-conjugated Antibody

Catalog #: IC1759F
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
IC1759F-100
IC1759F-025
Detection of Oct‑3/4 in BG01V Human Cells by Flow Cytometry.
2 Images
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Oct-3/4 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human Oct-3/4 in Western blots and detects mouse Oct-3/4 in flow cytometry.
Source
Monoclonal Rat IgG2B Clone # 240408
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Oct‑3/4
Met1-Asn265 (Met262Leu)
Accession # Q01860
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of Oct‑3/4 antibody in BG01V Human Cells antibody by Flow Cytometry. View Larger

Detection of Oct‑3/4 in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Rat Anti-Human/Mouse Oct-3/4 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC1759F, filled histogram) or isotype control antibody (Catalog # IC013F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry Detection of Oct‑3/4 antibody in Mouse Embryonic Stem Cells antibody by Flow Cytometry. View Larger

Detection of Oct‑3/4 in Mouse Embryonic Stem Cells by Flow Cytometry. Mouse embryonic stem cells treated with 5 µM Retinoic Acid for 2 days were stained with Rat Anti-Human/Mouse Oct-3/4 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC1759F, filled histogram) or isotype control antibody (Catalog # IC013F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Oct-3/4

Oct-3/4, also termed Oct-3 or Oct-4, is a POU transcription factor that is expressed in totipotent embryonic stem and germ cells.(2, 3) Oct-3/4 is required to sustain stem cell self-renewal and pluripotency.(4) It is considered a master regulator of pluripotency that controls lineage commitment and is the most widely recognized marker of totipotent embryonic stem cells.

References
  1. Takeda, J. et al. (1992) Nucleic Acids Res. 20:4613.
  2. Scholer, H.R. et al. (1989) EMBO J. 8:2543.
  3. Rosner, M.H. et al. (1990) Nature 345:686.
  4. Niwa, H. et al. (2000) Nat. Genet. 24:372.
Long Name
Octamer-binding Protein 3/4
Entrez Gene IDs
5460 (Human); 18999 (Mouse)
Alternate Names
3-Oct; Oct-3/4; OCT3;Oct-3;OCT4;Oct-4;OCT4;Oct3/4;OTF3;OTF-3;OTF4;POU domain protein;POU domain, class 5, transcription factor 1;POU5F1; Oct4; Otf3g; Otf4; Pou5f1

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human/Mouse Oct-3/4 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
Filter your results:

Filter by:

  1. Dental pulp pluripotent-like stem cells (DPPSC), a new stem cell population with chromosomal stability and osteogenic capacity for biomaterials evaluation
    Authors: R Núñez-Told, E Martínez-S, C Gil-Recio, MÁ Carrasco, A Al Madhoun, S Montori, M Atari
    BMC Cell Biol., 2017-04-21;18(1):21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Chemoresistant lung cancer stem cells display high DNA repair capability to remove cisplatin-DNA damage
    Authors: Wai-Kin Yu
    Br. J. Pharmacol, 2017-01-16;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. A human iPSC model of Ligase IV deficiency reveals an important role for NHEJ-mediated-DSB repair in the survival and genomic stability of induced pluripotent stem cells and emerging haematopoietic progenitors
    Authors: K Tilgner, I Neganova, I Moreno-Gimeno, J Y AL-Aama, D Burks, S Yung et al.
    Cell Death & Differentiation
  4. Interwoven Four-Compartment Capillary Membrane Technology for Three-Dimensional Perfusion with Decentralized Mass Exchange to Scale Up Embryonic Stem Cell Culture
    Authors: Jörg C. Gerlach, Marc Lübberstedt, Josefina Edsbagge, Alexander Ring, Mariah Hout, Matt Baun et al.
    Cells Tissues Organs
  5. Automated, scalable culture of human embryonic stem cells in feeder-free conditions.
    Authors: Thomas RJ, Anderson D, Chandra A, Smith NM, Young LE, Williams D, Denning C
    Biotechnol. Bioeng., 2009-04-15;102(6):1636-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human/Mouse Oct-3/4 Fluorescein-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse Oct-3/4 Fluorescein-conjugated Antibody and earn rewards!

Have you used Human/Mouse Oct-3/4 Fluorescein-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review