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Human/Mouse Myogenin Antibody

Catalog #: MAB6686
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6686
MAB6686-SP
Myogenin in C2C12 Mouse Myoblast Cells.
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Citations (1)
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Human/Mouse Myogenin Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human Myogenin in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 671038
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Myogenin
Ser124-Asn224
Accession # P15173
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Myogenin antibody in C2C12 Mouse Myoblast Cells by Immunocytochemistry (ICC). View Larger

Myogenin in C2C12 Mouse Myoblast Cells. Myogenin was detected in immersion fixed differentiated C2C12 mouse myoblast cells using Mouse Anti-Human Myogenin Monoclonal Antibody (Catalog # MAB6686) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to select differentiating nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Flow Cytometry View Larger

Detection of Myogenin in C2C12 cells by Flow Cytometry. C2C12 cells were stained with Mouse Anti-Human/Mouse Myogenin Monoclonal Antibody (Catalog # MAB6686, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Myogenin

Myogenin, also called MYF4, is a 34 kDa, 224 amino acid (aa) basic-HLH transcription factor member of the myogenic regulatory factor (MRF) family. It induces fibroblastic differentiation to myoblasts and is expressed most highly early in the skeletal muscle differentiation process. Within the region used as an immunogen, human myogenin shares 93% and 91% aa identity with mouse and rat myogenin, respectively. Isoforms may exist that diverge at aa 155 (246 aa form) or that are truncated at aa 215.

Entrez Gene IDs
4656 (Human); 17928 (Mouse); 29148 (Rat)
Alternate Names
BHLHC3; bHLHc3Myogenic factor-4; myogenin; Class C basic helix-loop-helix protein 3; MYF4; myf-4; MYF4myogenin; MYOG; Myogenic factor 4; myogenin (myogenic factor 4); Myogenin

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Citation for Human/Mouse Myogenin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Skeletal Muscle Resident Progenitor Cells Coexpress Mesenchymal and Myogenic Markers and Are Not Affected by Chronic Heart Failure-Induced Dysregulations
    Authors: RI Dmitrieva, TA Lelyavina, MY Komarova, VL Galenko, OA Ivanova, PA Tikanova, NV Khromova, AS Golovkin, MA Bortsova, A Sergushich, MY Sitnikova, AA Kostareva
    Stem Cells Int, 2019-01-03;2019(0):5690345.
    Species: Human
    Sample Types: Whole Tissue
    Applications: ICC

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