Human/Mouse IL-12/IL-35 p35 PerCP-conjugated Antibody

Name: Human/Mouse IL-12/IL-35 p35 PerCP-conjugated Antibody
Brand: R&D Systems
SKU: IC2191C
Rating: 5 (1 reviews)

Catalog #: IC2191C
1 Citation 1 Review Datasheet / COA / SDS

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IC2191C

Detection of IL‑12/IL‑35 p35 in Human PBMCs by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse IL-12/IL-35 p35 PerCP-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.

Source

Monoclonal Mouse IgG₁ Clone # 27537

Purification

Protein A or G purified from ascites

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12/IL-35 p35
Arg23-Ser219
Accession # P29459

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of IL-12/IL-35 p35 antibody in Human PBMCs antibody by Flow Cytometry.

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Detection of IL‑12/IL‑35 p35 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either untreated (light orange filled histogram) or treated with LPS (dark orange filled histogram) were stained with Mouse Anti-Human/Mouse IL-12/IL-35 p35 PerCP-conjugated Monoclonal Antibody (Catalog # IC2191C) or isotype control antibody (Catalog # IC002C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry

Detection of IL-12/IL-35 p35 antibody in Mouse Splenocytes antibody by Flow Cytometry.

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Detection of IL‑12/IL‑35 p35 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either untreated (light orange filled histogram) or treated with LPS (dark orange filled histogram) were stained with Mouse Anti-Human/Mouse IL-12/IL-35 p35 PerCP-conjugated Monoclonal Antibody (Catalog # IC2191C) or isotype control antibody (Catalog # IC002C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-12/IL-35 p35

Interleukin 12 (IL-12) and interleukin 35 (IL-35) are heterodimeric cytokines composed of alpha and beta chains. IL-12 is composed of p35 and p40 subunits, while IL-35 is comprised of p35 paired with EBI-3 (1). In mice, IL-35 is produced by FoxP3⁺ regulatory T cells and may function as an inhibitory cytokine to suppress T cell proliferation (2). Human FoxP3⁺ Tregs do not constitutively express IL-35 (3), but expression may be induced by activated dendritic cells (4).

References

Collison, L.W. and D.A.A. Vignali (2008) Immunol. Rev. 226:248.
Collison, L.W. et al. (2007) Nature 450:566.
Bardel, E. et al. (2008) J. Immunol. 181:6898.
Seyerl, M. et al.(2010) Eur. J. Immunol. 40:321.

Long Name

Interleukin 12 p35

Entrez Gene IDs

3592 (Human); 16159 (Mouse); 84405 (Rat)

Alternate Names

CLMF p35; CLMF; cytotoxic lymphocyte maturation factor 1, p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12, subunit p35; IL-12/IL-35 p35; IL-12A; IL35 subunit; IL35p35; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; NF cell stimulatory factor chain 1; NFSK; NK cell stimulatory factor chain 1; NKSF1; p35

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Human/Mouse IL-12/IL-35 p35 PerCP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1