Human/Mouse HO-1/HMOX1/HSP32 Antibody Summary
Met1-Thr261
Accession # P09601
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse HO‑1/HMOX1/HSP32 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse HO-1/HMOX1/HSP32 Monoclonal Antibody (Catalog # MAB3776) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for HO-1/HMOX1/HSP32 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
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Western Blot Shows Human HO‑1/HMOX1/HSP32 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HO-1/HMOX1/HSP32 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse HO-1/HMOX1/HSP32 Monoclonal Antibody (Catalog # MAB3776) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for HO-1/HMOX1/HSP32 at approximately 32 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HO-1/HMOX1/HSP32
Heme Oxygenase 1 (HO-1), also known as HMOX1 and Heat Shock Protein 32 (HSP32), is a 32 kDa microsomal enzyme required for the metabolism of heme to biliverdin. Heme Oxygenase occurs as 2 isozymes, an inducible Heme Oxygenase-1 (HO-1/HMOX1) and a constitutive Heme Oxygenase-2 (HO-2/HMOX2). HO-1 expression is induced by heme and other non-heme compounds. Human HO-1 shares 82% amino acid sequence identity with mouse HO-1.
Product Datasheets
Citations for Human/Mouse HO-1/HMOX1/HSP32 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Inner ear supporting cells protect hair cells by secreting HSP70
Authors: Lindsey A. May, Inga I. Kramarenko, Carlene S. Brandon, Christina Voelkel-Johnson, Soumen Roy, Kristy Truong et al.
Journal of Clinical Investigation
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?2-AR inhibition enhances EGFR antibody efficacy hampering the oxidative stress response machinery
Authors: Del Vecchio, V;Mele, L;Panda, SK;Sanchez-Pajares, IR;Mosca, L;Tirino, V;Barbieri, M;Bruzzese, F;Luciano, A;Marino, FZ;Accardo, M;Nicoletti, GF;Papaccio, G;Barbieri, A;Desiderio, V;
Cell death & disease
Species: Xenograft
Sample Types: Whole Tissue
Applications: IHC -
Dysregulation of Nrf2 redox pathway in macrophages under diabetic microenvironment
Authors: GV Ganesh, KM Ramkumar
Experimental gerontology, 2021-07-10;152(0):111479.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Mitochondrial alterations triggered by repeated exposure to fine (PM2.5-0.18) and quasi-ultrafine (PM0.18) fractions of ambient particulate matter
Authors: J Sotty, J Kluza, C De Sousa, M Tardivel, S Anthérieu, LY Alleman, L Canivet, E Perdrix, A Loyens, P Marchetti, JM Lo Guidice, G Garçon
Environ Int, 2020-06-22;142(0):105830.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Induction of heme oxygenase-1 in factor VIII-deficient mice reduces the immune response to therapeutic factor VIII.
Authors: Dimitrov JD, Dasgupta S, Navarrete AM, Delignat S, Repesse Y, Meslier Y, Planchais C, Teyssandier M, Motterlini R, Bayry J, Kaveri SV, Lacroix-Desmazes S
Blood, 2009-11-04;115(13):2682-5.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Heat Shock Protein-Mediated Protection Against Cisplatin-Induced Hair Cell Death
Authors: Tiffany G. Baker, Soumen Roy, Carlene S. Brandon, Inga K. Kramarenko, Shimon P. Francis, Mona Taleb et al.
Journal of the Association for Research in Otolaryngology
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Heme Drives Susceptibility of Glomerular Endothelium to Complement Overactivation Due to Inefficient Upregulation of Heme Oxygenase-1
Authors: Olivia May, Nicolas S. Merle, Anne Grunenwald, Viviane Gnemmi, Juliette Leon, Cloé Payet et al.
Frontiers in Immunology
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