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Human/Mouse FoxP3 Antibody

Catalog #: MAB8214
19 Citations 1 Review Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB8214
MAB8214-SP
Detection of FOXP3 in Hodgkin's Lymphoma via Multiplex Immunofluorescence staining on COMET™
11 Images
Product Details
Citations (19)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse FoxP3 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human FoxP3 in direct ELISAs. Detects human and mouse FoxP3 in flow cytometry.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1054C
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human FoxP3
Met1-Leu71
Accession # Q9BZS1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
5-25 µg/mL
Immersion fixed paraffin-embedded sections of human tonsil, human ovarian cancer tissue, and mouse thymus tissue
Dual RNAscope ISH-IHC
5-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below
Multiplex Immunofluorescence
20 µg/mL
Immersion fixed paraffin-embedded sections of Hodgkin's Lymphoma and Mouse Thymus

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Multiplex Immunofluorescence View Larger

Detection of FOXP3 in Hodgkin's Lymphoma via Multiplex Immunofluorescence staining on COMET™ FOXP3 was detected in immersion fixed paraffin-embedded sections of Hodgkin's Lymphoma using Rabbit Anti-Human FOXP3 Monoclonal Antibody (Catalog # MAB8214) at 20 µg/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the nucleus. Protocol available in COMET™ Panel Builder.

Multiplex Immunofluorescence View Larger

Detection of FOXP3 in Mouse Thymus via seqIF™ staining on COMET™​ FOXP3 Antibody was detected in immersion fixed paraffin-embedded sections of mouse Thymus using Rabbit Anti-Mouse FOXP3 Monoclonal Antibody (Catalog # MAB8214) at 20ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the nucleus. Protocol available in COMET™ Panel Builder.

Flow Cytometry Detection of FoxP3 antibody in Human PBMCs stimulated to induce Tregs antibody by Flow Cytometry. View Larger

Detection of FoxP3 in Human PBMCs stimulated to induce Tregs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) stimulated to induce Regulatory T Cells (Tregs) with Recombinant Human TGF-beta 1 (Catalog # 240-B) and Recombinant Human IL-2 (Catalog # 202-IL) for 2 days were stained with Rabbit Anti-Human/Mouse FoxP3 Monoclonal Antibody (Catalog # MAB8214) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110) and Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A). Quadrant markers were set based on control antibody staining (Catalog # AB-105-C). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Flow Cytometry Detection of FoxP3 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of FoxP3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A) and either (A) Rabbit Anti-Human/Mouse FoxP3 Monoclonal Antibody (Catalog # MAB8214) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

Immunocytochemistry FoxP3 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

FoxP3 in HeLa Human Cell Line. FoxP3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human/Mouse FoxP3 Monoclonal Antibody (Catalog # MAB8214) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red, upper panel; Catalog # NL004) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry FoxP3 antibody in Human Tonsil by Immunohistochemistry (IHC-P). View Larger

FoxP3 in Human Tonsil. FoxP3 was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human/Mouse FoxP3 Monoclonal Antibody (Catalog # MAB8214) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry FoxP3 antibody in Human Ovarian Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

FoxP3 in Human Ovarian Cancer Tissue. FoxP3 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Rabbit Anti-Human/Mouse FoxP3 Monoclonal Antibody (Catalog # MAB8214) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Dual RNAscope ISH-IHC Compatible FoxP3 antibody in Human Tonsil Using Dual RNAscope<sup>®</sup>ISH and IHC. View Larger

FoxP3 in Human Tonsil Using Dual RNAscope®ISH and IHC. FoxP3 mRNA (red) and protein (green) was detected in formalin-fixed paraffin-embedded tissue sections of human tonsil probed with ACD RNAScope®Probe (Catalog # 418471) followed by immunohistochemistry using R&D Systems Rabbit Anti-Human/Mouse FoxP3 Monoclonal Antibody (Catalog# MAB8214) at 5ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC003). Tissue was stained using ACD RNAscope®2.5 HD Duplex Detection Reagents (Catalog # 322500).

Immunocytochemistry/ Immunofluorescence Detection of Human FoxP3 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human FoxP3 by Immunocytochemistry/Immunofluorescence Multiplex fluorescent immunohistochemistry staining. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31192136), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Human FoxP3 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Human FoxP3 by Immunocytochemistry/Immunofluorescence Multiplex fluorescent immunohistochemistry staining. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31192136), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry View Larger

Detection of FoxP3 in Mouse Thymus. FoxP3 was detected in immersion fixed paraffin-embedded sections of mouse thymus using Rabbit Anti-Human/Mouse FoxP3 Monoclonal Antibody (Catalog # MAB8214) at 15 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FoxP3

Human FoxP3 is a 47 kDa member of the P subclass of the FOX (forkhead box) family of transcription factors. It contains a Leu-rich repeat, a C2H2 zinc finger region, and a C-terminal FKH (fork head), DNA-binding domain. Three isoforms for FoxP3 have been reported. All three isoforms share the sequence used as the immunogen. FoxP3 directly associates with NFAT and NFkB, suppressing their activity in CD4+ T cells. In human, FoxP3 is found in CD4+, CD8+ and CD4+CD25+ T cells. Over the region used for immunization of the amino acid sequence, mouse FoxP3 is 83% to 88% identical to rat, human, canine, and bovine FoxP3.

Long Name
Forkhead Box P3
Entrez Gene IDs
50943 (Human); 20371 (Mouse)
Alternate Names
AIID; AIIDMGC141961; DIETER; forkhead box P3; Forkhead Box Protein P3; FoxP3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked; IPEX; JM2; MGC141961; MGC141963; PIDX; PIDXMGC141963; SCURFIN; XPID; XPIDpolyendocrinopathy, enteropathy, X-linked

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Citations for Human/Mouse FoxP3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. NOD mice, susceptible to pancreatic autoimmunity, demonstrate delayed growth of pancreatic cancer.
    Authors: Dooley J, Pasciuto E, Lagou V, Lampi Y, Dresselaers T, Himmelreich U, Liston A
    Oncotarget, 2017-09-24;8(46):80167-80174.
  2. Microglia Require CD4 T Cells to Complete the Fetal-to-Adult Transition
    Authors: Emanuela Pasciuto, Oliver T. Burton, Carlos P. Roca, Vasiliki Lagou, Wenson D. Rajan, Tom Theys et al.
    Cell
  3. Genome-scale in vivo CRISPR screen identifies RNLS as a target for beta cell protection in type 1 diabetes
    Authors: Cai EP, Ishikawa Y, Zhang W et al.
    Nat Metab
  4. Mesothelial cell-derived antigen-presenting cancer-associated fibroblasts induce expansion of regulatory T cells in pancreatic cancer
    Authors: Huocong Huang, Zhaoning Wang, Yuqing Zhang, Rachana N. Pradhan, Debolina Ganguly, Raghav Chandra et al.
    Cancer Cell
  5. Astrocyte-targeted gene delivery of interleukin 2 specifically increases brain-resident regulatory T cell numbers and protects against pathological neuroinflammation
    Authors: Lidia Yshii, Emanuela Pasciuto, Pascal Bielefeld, Loriana Mascali, Pierre Lemaitre, Marika Marino et al.
    Nature Immunology
  6. Near-infrared photoimmunotherapy targeting human-EGFR in a mouse tumor model simulating current and future clinical trials
    Authors: Ryuhei Okada, Aki Furusawa, Daniel W. Vermeer, Fuyuki Inagaki, Hiroaki Wakiyama, Takuya Kato et al.
    EBioMedicine
  7. Integrated Analysis Highlights the Immunosuppressive Role of TREM2+ Macrophages in Hepatocellular Carcinoma
    Authors: Lisha Zhou, Meiling Wang, Hanrui Guo, Jun Hou, Yingna Zhang, Man Li et al.
    Frontiers in Immunology
  8. PRSS2 remodels the tumor microenvironment via repression of Tsp1 to stimulate tumor growth and progression
    Authors: L Sui, S Wang, D Ganguly, TP El Rayes, C Askeland, A Børretzen, D Sim, OJ Halvorsen, G Knutsvik, J Arnes, S Aziz, S Haukaas, WD Foulkes, DR Bielenberg, A Ziemys, V Mittal, RA Brekken, LA Akslen, RS Watnick
    Nature Communications, 2022-12-27;13(1):7959.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Tumor-infiltrated activated B cells suppress liver metastasis of colorectal cancers
    Authors: Y Xu, Z Wei, M Feng, D Zhu, S Mei, Z Wu, Q Feng, W Chang, M Ji, C Liu, Y Zhu, L Shen, F Yang, Y Chen, Y Feng, J Xu, D Zhu
    Cell Reports, 2022-08-30;40(9):111295.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. VEGFR2 activity on myeloid cells mediates immune suppression in the tumor microenvironment
    Authors: Y Zhang, H Huang, M Coleman, A Ziemys, P Gopal, SM Kazmi, RA Brekken
    JCI Insight, 2021-12-08;6(23):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Dissecting spatial heterogeneity and the immune-evasion mechanism of CTCs by single-cell RNA-seq in hepatocellular carcinoma
    Authors: YF Sun, L Wu, SP Liu, MM Jiang, B Hu, KQ Zhou, W Guo, Y Xu, Y Zhong, XR Zhou, ZF Zhang, G Liu, S Liu, YH Shi, Y Ji, M Du, NN Li, GB Li, ZK Zhao, XY Huang, LQ Xu, QC Yu, DH Peng, SJ Qiu, HC Sun, M Dean, XD Wang, WY Chung, AR Dennison, J Zhou, Y Hou, J Fan, XR Yang
    Nature Communications, 2021-07-02;12(1):4091.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  12. Regulatory T-cell expansion in oral and maxillofacial Langerhans cell histiocytosis
    Authors: C Zhang, J Gao, J He, C Liu, X Lv, X Yin, Y Deng, Z Lu, Z Tian
    Oral Surg Oral Med Oral Pathol Oral Radiol, 2020-08-08;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  13. Formation of Human Neuroblastoma in Mouse-Human Neural Crest Chimeras
    Authors: MA Cohen, S Zhang, S Sengupta, H Ma, GW Bell, B Horton, B Sharma, RE George, S Spranger, R Jaenisch
    Cell Stem Cell, 2020-03-05;0(0):.
    Species: Mouse
    Sample Types: Tissue
    Applications: IHC-P
  14. Improved Multiplex Immunohistochemistry for Immune Microenvironment Evaluation of Mouse Formalin-Fixed, Paraffin-Embedded Tissues
    Authors: N Sorrelle, D Ganguly, ATA Dominguez, Y Zhang, H Huang, LN Dahal, N Burton, A Ziemys, RA Brekken
    J. Immunol., 2018-12-03;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. CD25 and TGF-? blockade based on predictive integrated immune ratio inhibits tumor growth in pancreatic cancer
    Authors: N Pu, G Zhao, H Yin, JA Li, A Nuerxiati, D Wang, X Xu, T Kuang, D Jin, W Lou, W Wu
    J Transl Med, 2018-10-25;16(1):294.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Mice transgenic for human CTLA4-CD28 fusion gene show proliferation and transformation of ATLL-like and AITL-like T cells
    Authors: Gyu Jin Lee, Yukyung Jun, Yoon Kyung Jeon, Daekee Lee, Sanghyuk Lee, Jaesang Kim
    OncoImmunology
  17. Targeting TGFbetaR2-mutant tumors exposes vulnerabilities to stromal TGFbeta blockade in pancreatic cancer
    Authors: H Huang, Y Zhang, V Gallegos, N Sorrelle, MM Zaid, J Toombs, W Du, S Wright, M Hagopian, Z Wang, AN Hosein, AA Sathe, C Xing, EJ Koay, KE Driscoll, RA Brekken
    EMBO Mol Med, 2019-10-14;0(0):e10515.
  18. Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer
    Authors: Choi YW, Kim YH, Oh SY et al.
    Advanced Science
  19. RNU (Foxn1 RNU-Nude) Rats Demonstrate an Improved Ability to Regenerate Muscle in a Volumetric Muscle Injury Compared to Sprague Dawley Rats
    Authors: Michael J. McClure, Lucas C. Olson, David J. Cohen, Yen Chen Huang, Shirley Zhang, Tri Nguyen et al.
    Bioengineering (Basel)

FAQs

  1. What detection reagent was used for IHC, to create the Dual RNAscope ISH-IHC image on the datasheet for Catalog # MAB8214? 

    • Green Chromogen from ACD (ACD Catalog # 322550) was ued for IHC detection. See ACD website (https://acdbio.com/store/catalog/product/view/id/22/) for protocol and handling recommendations.  Extensive washing with buffers containing PBS is discouraged as it may cause decolorization. It is critical that after staining with green chromogen, slides with tissues are allowed to dry at room temperature prior to using a permanent mounting media.

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Human/Mouse FoxP3 Antibody
By Anonymous on 05/25/2017
Application: WB Sample Tested: MCF-7 human breast cancer cell line Species: Human