Human/Mouse CLEC10A/CD301 Antibody Summary
Gln61-His316
Accession # Q8IUN9
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human CLEC10A/CD301 by Western Blot. Western blot shows lysates of human immature dendritic cells and human mature dendritic cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse CLEC10A/CD301 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4888) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CLEC10A/CD301 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of CLEC10A in Human Dendritic Cells by Flow Cytometry. Human immature dendritic cells were stained with Goat Anti-Human/Mouse CLEC10A/CD301 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4888, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
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Detection of Mouse CLEC10A/CD301 by Western Blot. Western blot shows lysates of mouse immature dendritic cells and mouse mature dendritic cells. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse CLEC10A/CD301 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4888) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CLEC10A/CD301 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CLEC10A/CD301
CLEC10A, also known as macrophage galactose/N-acetyl-galactosamine (GalNAc) specific lectin (MGL), CD301, DC-ASGPR, and HML, is a 35-40 kDa type II transmembrane glycoprotein that belongs to the C-type lectin family (1). Human and rat carry a single gene for CLEC10A/MGL, while mouse has two closely related MGL1 and MGL2 genes. Human CLEC10A/MGL consists of a 39 amino acid (aa) cytoplasmic region, a 21 aa transmembrane segment and a 256 aa extracellular domain (ECD) with one carbohydrate recognition domain (CRD) and a neck region (2). Within the CRD, human CLEC10A/MGL shares 64% - 70% aa sequence identity with mouse MGL1, mouse MGL2, and rat MGL. Alternate splicing generates multiple isoforms of human CLEC10A/MGL with 27 aa, 3 aa, and/or 4 aa deletions within the ECD (3, 4). CLEC10A/MGL is expressed on immature myleloid dendritic cells and alternatively activated (tolerogenic) macrophages and is upregulated by the immunosuppressant dexamethasone (3-7). CLEC10A/MGL selectively binds and internalizes terminal nonsialylated alpha - or beta -linked GalNAc moieties on O-linked carbohydrates, including the Tn carcinoma antigen (2-4, 8, 9). Similar ligand preference is exhibited by mouse MGL2 but not MGL1 (10). CLEC10A/MGL expressed on tolerogenic dendritic cells binds carbohydrate determinants on CD45 (RA, RB, and RC but not RO isoforms) expressed by T, NK, and B cells (6). This interaction inhibits effector T cell activation and induces their apoptosis (6). CLEC10A/MGL also binds the GP envelope glycoprotein on Marburg and Ebola viruses and enhances viral entry and infectivity (11).
- Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
- Suzuki, N. et al. (1996) J. Immunol. 156:128.
- Higashi, N. et al. (2002) J. Biol. Chem. 277:20686.
- Valladeau, J. et al. (2001) J. Immunol. 167:5767.
- van Vliet, S.J. et al. (2006) Immunobiology 211:577.
- van Vliet, S.J. et al. (2006) Nat. Immunol. 7:1200.
- Raes, G. et al. (2005) J. Leukoc. Biol. 77:321.
- van Vliet, S.J. et al. (2005) Int. Immunol. 17:661.
- Higashi, N. et al. (2002) Int. Immunol. 14:545.
- Tsuiji, M. et al. (2002) J. Biol. Chem. 277:28892.
- Takada, A. et al. (2004) J. Virol. 78:2943.
Product Datasheets
Citations for Human/Mouse CLEC10A/CD301 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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C1GalT1 expression reciprocally controls tumour cell-cell and tumour-macrophage interactions mediated by galectin-3 and MGL with double impact on cancer development and progression
Authors: Wan, Y;Adair, K;Herrmann, A;Shan, X;Xia, L;Duckworth, CA;Yu, LG;
Cell death & disease
Species: Human, Transgenic Mouse
Sample Types: Cell Lysates, Transfected Whole Cells, Whole Tissue
Applications: Immunoprecipitation, IHC, Flow Cytometry -
CD301/CLEC10 Expression and Its Role in Il-4 Driven Multinucleated Giant Cell Formation
Authors: Brooks PJ
Thesis
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