Human/Mouse CBL Antibody

Catalog #: MAB5998 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5998
MAB5998-SP

Detection of Human and Mouse CBL by Western Blot.

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Human/Mouse CBL Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse CBL in Western blots.

Source

Monoclonal Mouse IgG_2A Clone # 648005

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human CBL
Asp775-Thr906
Accession # P22681

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Simple Western

10 µg/mL

Daudi human Burkitt's lymphoma cells, K562 human chronic myelogenous leukemia cells, Raji human Burkitt's lymphoma cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse CBL by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, Raji human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse CBL Monoclonal Antibody (Catalog # MAB5998) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for CBL at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human/Mouse CBL by Simple Western^TM. Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cells, K562 human chronic myelogenous leukemia cells, Raji human Burkitt's lymphoma cells, loaded at 0.2 mg/mL. A specific band was detected for CBL at approximately 116 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse CBL Monoclonal Antibody (Catalog # MAB5998). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CBL

CBL (Lake Casitas, California mouse B-lineage Lymphoma; also RING finger protein 55) is a 120‑125 kDa cytosolic member of the CBL family of proteins. It is widely expressed, being found in cells such as fibroblasts, adipocytes, osteoclasts, podocytes and mammary epithelium. CBL negatively regulates RTK signaling by acting as an E3 ubiquitin ligase. Following activation via phosphorylation, CBL will interact with multiple signaling molecules such as Src, ZAP-70, EGFR and SHP1. Human CBL is 906 amino acids (aa) in length. It contains an N-terminal phosphoTyr-binding region that consists of one alpha -helix bundle, an EF hand segment, and an SH2 domain (aa 47‑342), a RING finger domain that interacts with E2 enzymes (aa 381‑423) a Pro-rich SH3 domain (aa 477‑688), and a Leu‑zipper/Ubiquitin‑associated region (aa 861‑892). There is one potential alternative start site at Met55. Over aa 775‑906, human CBL shares 91% aa identity with mouse CBL.

Long Name

Casitas B-lineage Lymphoma, E3 Ubiquitin Protein Ligase CBL

Entrez Gene IDs

867 (Human); 12402 (Mouse); 500985 (Rat)

Alternate Names

Cas-Br-M (murine) ecotropic retroviral transforming sequence; Casitas B-lineage lymphoma proto-oncogene; CBL; CBL2; CBL2E3 ubiquitin-protein ligase CBL; EC 6.3.2; EC 6.3.2.-; oncogene CBL2; Proto-oncogene c-Cbl; RING finger protein 55; RNF55; RNF55C-CBL; Signal transduction protein CBL