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Human/Mouse BDNF DuoSet ELISA

Catalog #: DY248
75 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY248
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Human / Mouse BDNF ELISA Standard Curve
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Citations (75)
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Reviews (2)
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human/Mouse BDNF DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
23.4 - 1,500 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human BDNF. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

 

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A Human / Mouse BDNF ELISA Standard Curve View Larger

Human / Mouse BDNF ELISA Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: BDNF

BDNF (Brain-Derived Neurotrophic Factor) is a secreted protein that regulates many aspects of neuronal development and function in the nervous system. It is produced as proBDNF in response to neuronal activity or inflammatory stimulation and is then cleaved before associating into a homodimer. It is expressed throughout the nervous system as well as by fibroblasts, megakaryocytes/platelets, and smooth muscle cells. BDNF regulates neural stem cell survival and differentiation, axon/dendrite differentiation, synapse formation and maturation, and refinement of developing circuits. It is also a key regulator of synaptic plasticity and late-phase long-term potentiation. BDNF signals through the TrkB and NGF R/TNFRSF16 receptors. Secreted proBDNF can bind to NGF R/TNFRSF16 and induce long-term depression and cell apoptosis.

Long Name:
Brain-derived Neurotrophic Factor
Entrez Gene IDs:
627 (Human); 12064 (Mouse); 24225 (Rat)
Alternate Names:
Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

 

Citations for Human/Mouse BDNF DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

75 Citations: Showing 1 - 10
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  1. Neuroprotective effects of intranasal extracellular vesicles from human platelet concentrates supernatants in traumatic brain injury and Parkinson's disease models
    Authors: Delila, L;Nebie, O;Le, NTN;Timmerman, K;Lee, DY;Wu, YW;Chou, ML;Buée, L;Chou, SY;Blum, D;Devos, D;Burnouf, T;
    Journal of biomedical science
    Species: Human
    Sample Types: Extracellular Vesicles
  2. Controlled Hypoxia Acutely Prevents Physical Inactivity-Induced Peripheral BDNF Decline
    Authors: Duderstadt, Y;Schreiber, S;Burtscher, J;Schega, L;Müller, NG;Brigadski, T;Braun-Dullaeus, RC;Le beta mann, V;Müller, P;
    International journal of molecular sciences
    Species: Human
    Sample Types: Serum, Plasma
  3. Baseline circulating biomarkers, their changes, and subsequent suicidal ideation and depression severity at 6 months: A prospective analysis in patients with mood disorders
    Authors: Lengvenyte, A;Cognasse, F;Hamzeh-Cognasse, H;Sénèque, M;Strumila, R;Olié, E;Courtet, P;
    Psychoneuroendocrinology
    Species: Human
    Sample Types: Plasma
  4. BDNF-loaded PDADMAC-heparin multilayers: a novel approach for neuroblastoma cell study
    Authors: D?bkowska, M;Stukan, I;Kowalski, B;Donerowicz, W;Wasilewska, M;Szatanik, A;Sta?czyk-Dunaj, M;Michna, A;
    Scientific reports
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Chronic hyperpalatable diet induces impairment of hippocampal-dependent memories and alters glutamatergic and fractalkine axis signaling
    Authors: Ribeiro, R;Silva, EG;Moreira, FC;Gomes, GF;Cussat, GR;Silva, BSR;da Silva, MCM;de Barros Fernandes, H;de Sena Oliveira, C;de Oliveira Guarnieri, L;Lopes, V;Ferreira, CN;de Faria, AMC;Maioli, TU;Ribeiro, FM;de Miranda, AS;Moraes, GSP;de Oliveira, ACP;Vieira, LB;
    Scientific reports
    Species: Mouse
    Sample Types: Serum, Tissue Homogenates
  6. Changes in Adipokine, Resitin, and BDNF Concentrations in Treatment-Resistant Depression after Electroconvulsive Therapy
    Authors: Permoda-Pachuta, A;Malewska-Kasprzak, M;Skibi?ska, M;Rzepski, K;Dmitrzak-W?glarz, M;
    Brain sciences
    Species: Human
    Sample Types: Serum
  7. Cefaclor causes vagus nerve-mediated depression-like symptoms with gut dysbiosis in mice
    Authors: Joo, MK;Shin, YJ;Kim, DH;
    Scientific reports
    Species: Mouse
    Sample Types: Tissue Homogenates
  8. The relationship between the brain-derived neurotrophic factor and neurocognitive response to physical exercise in individuals with schizophrenia
    Authors: Bang-Kittilsen, G;Egeland, J;Ueland, T;Andersen, E;Bigseth, TT;Holmen, TL;Mordal, J;Holst, R;Engh, JA;
    Psychoneuroendocrinology
    Species: Human
    Sample Types: Platelet-Poor Plasma
  9. Inflammatory biomarkers at different stages of Sarcopenia in older women
    Authors: da Costa Teixeira, LA;Avelar, NCP;Peixoto, MFD;Parentoni, AN;Santos, JMD;Pereira, FSM;Danielewicz, AL;Leopoldino, AAO;Costa, SP;Arrieiro, AN;Soares, LA;da Silva Lage, VK;Prates, ACN;Taiar, R;de Carvalho Bastone, A;Oliveira, VC;Oliveira, MX;Costa, HS;Nobre, JNP;Brant, FP;Duarte, TC;Figueiredo, PHS;Mendonça, VA;Lacerda, ACR;
    Scientific reports
    Species: Human
    Sample Types: Plasma
  10. Mild Cognitive Impairment Is Associated with Enhanced Activation of Th17 Lymphocytes in Non-Alcoholic Fatty Liver Disease
    Authors: Fiorillo, A;Gallego, JJ;Casanova-Ferrer, F;Giménez-Garzó, C;Urios, A;Ballester, MP;Durbán, L;Rios, MP;Megías, J;San Miguel, T;Kosenko, E;Escudero-García, D;Benlloch, S;Felipo, V;Montoliu, C;
    International journal of molecular sciences
    Species: Human
    Sample Types: Plasma
  11. Omega-3 polyunsaturated fatty acids (n-3 PUFAs), somatic and fatigue symptoms in cardiovascular diseases comorbid major depressive disorder (MDD): A randomized controlled trial
    Authors: Chang, JP;Chang, SS;Chen, HT;Chien, YC;Yang, HT;Huang, SY;Tseng, PT;Chang, CH;Galecki, P;Su, KP;
    Brain, behavior, and immunity
    Species: Human
    Sample Types: Plasma
  12. Composite Coatings Based on Recombinant Spidroins and Peptides with Motifs of the Extracellular Matrix Proteins Enhance Neuronal Differentiation of Neural Precursor Cells Derived from Human Induced Pluripotent Stem Cells
    Authors: EV Novosadova, OV Dolotov, LV Novosadova, LI Davydova, KV Sidoruk, EL Arsenyeva, DM Shimchenko, VG Debabov, VG Bogush, VZ Tarantul
    International Journal of Molecular Sciences, 2023-03-02;24(5):.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Effects of Exercise Training on Neurotrophic Factors and Blood-Brain Barrier Permeability in Young-Old and Old-Old Women
    Authors: SY Cho, HT Roh
    International Journal of Environmental Research and Public Health, 2022-12-16;19(24):.
    Species: Human
    Sample Types: Serum
  14. Adiponectin Is a Contributing Factor of Low Appendicular Lean Mass in Older Community-Dwelling Women: A Cross-Sectional Study
    Authors: LAC Teixeira, JM Dos Santos, AN Parentoni, LP Lima, TC Duarte, FP Brant, CDC Neves, FSM Pereira, NCP Avelar, AL Danielewic, AAO Leopoldino, SP Costa, AN Arrieiro, LA Soares, ACN Prates, JNP Nobre, A de Carvalh, VC de Oliveir, MX Oliveira, PH Scheidt Fi, HS Costa, V Amaral Men, R Taiar, AC Rodrigues
    Journal of Clinical Medicine, 2022-12-02;11(23):.
    Species: Human
    Sample Types: Plasma
  15. Chronic Methylmercury Intoxication Induces Systemic Inflammation, Behavioral, and Hippocampal Amino Acid Changes in C57BL6J Adult Mice
    Authors: TS Nascimento, DV Pinto, RP Dias, RS Raposo, PIG Nunes, CR Roque, FA Santos, GM Andrade, JL Viana, AH Fostier, A Sussulini, JI Alvarez-Le, C Fontes-Rib, JO Malva, RB Oriá
    International Journal of Molecular Sciences, 2022-11-10;23(22):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  16. Circulating and Salivary NGF and BDNF Levels in SARS-CoV-2 Infection: Potential Predictor Biomarkers of COVID-19 Disease-Preliminary Data
    Authors: F Biamonte, A Re, BO Balzamino, G Ciasca, D Santucci, C Napodano, G Nocca, A Fiorita, M Marino, U Basile, A Micera, CAM Callà
    Journal of personalized medicine, 2022-11-09;12(11):.
    Species: Human
    Sample Types: Serum
  17. Gentle Touch Therapy, Pain Relief and Neuroplasticity at Baseline in Fibromyalgia Syndrome: A Randomized, Multicenter Trial with Six-Month Follow-Up
    Authors: ASI Salgado, MH Takemoto, CFTC de Souza, DC Salm, D da Rosa, GC Cardoso, DD Ludtke, SFC Soares, JK Ferreira, AR Dutra, YC Szeremeta, G Mazzardo, J Mayra, DDL Sheffer, W Caumo, EB Bittencour, R Schleip, A Latini, F Bobinski, DF Martins
    Journal of Clinical Medicine, 2022-08-20;11(16):.
    Species: Human
    Sample Types: Serum
  18. BDNF, proBDNF and IGF-1 serum levels in na�ve and medicated subjects with autism
    Authors: MLA Robinson-A, B Michalski, B Vidal-Mart, LR Hernández, MW Santiesteb, M Fahnestock
    Scientific Reports, 2022-08-12;12(1):13768.
    Species: Human
    Sample Types: Serum
  19. Human Primary Astrocytes Differently Respond to Pro- and Anti-Inflammatory Stimuli
    Authors: P Szpakowski, D Ksiazek-Wi, M Turniak-Ku, I Pacan, A Glabinski
    Biomedicines, 2022-07-22;10(8):.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Celastrol alleviates high-fat diet-induced obesity via enhanced muscle glucose utilization and mitochondrial oxidative metabolism-mediated upregulation of pyruvate dehydrogenase complex
    Authors: MHA Bakar, NSN Shahril, MSFM Khalid, S Mohammad, KA Shariff, T Karunakara, RM Salleh, MN Rosdi
    Oncogene, 2022-06-06;449(0):116099.
    Species: Mouse
    Sample Types: Serum
  21. DHA-Enriched Fish Oil Ameliorates Deficits in Cognition Associated with Menopause and the APOE4 Genotype in Rodents
    Authors: MG Pontifex, A Martinsen, RNM Saleh, G Harden, C Fox, M Muller, D Vauzour, AM Minihane
    Nutrients, 2022-04-19;14(9):.
    Species: Mouse
    Sample Types: Serum
  22. ProBDNF as an Indicator of Improvement among Women with Depressive Episodes
    Authors: W Zwoli?ska, M Skibinska, A S?opie?, M Dmitrzak-W
    Metabolites, 2022-04-16;12(4):.
    Species: Human
    Sample Types: Serum
  23. Endothelial Unc5B controls blood-brain barrier integrity
    Authors: K Boyé, LH Geraldo, J Furtado, L Pibouin-Fr, M Poulet, D Kim, B Nelson, Y Xu, L Jacob, N Maissa, D Agalliu, L Claesson-W, SL Ackerman, A Eichmann
    Nature Communications, 2022-03-04;13(1):1169.
    Species: Mouse
    Sample Types: EDTA Plasma
  24. Circulating Interleukin-6 and CD16 positive monocytes increase following angioplasty of an arteriovenous fistula
    Authors: S Hakki, EJ Robinson, MG Robson
    Scientific Reports, 2022-01-26;12(1):1427.
    Species: Human
    Sample Types: Plasma
  25. Comparison of the effects of open vs. closed skill exercise on the acute and chronic BDNF, IGF-1 and IL-6 response in older healthy adults
    Authors: T Behrendt, F Kirschnick, L Kröger, P Beileke, M Rezepin, T Brigadski, V Le beta mann, L Schega
    Bmc Neuroscience, 2021-11-25;22(1):71.
    Species: Human
    Sample Types: Plasma
  26. Apathy is not associated with a panel of biomarkers in older adults with HIV disease
    Authors: SP Woods, JL Thompson, MA Babicz, L Shahani, GD Colpo, NP Rocha, EE Morgan, AL Teixeira
    Journal of psychosomatic research, 2021-11-08;152(0):110666.
    Species: Human
    Sample Types: Plasma
  27. The Restorative Effect of Human Amniotic Fluid Stem Cells on Spinal Cord Injury
    Authors: M Lale Ataei, M Karimipour, P Shahabi, R Pashaei-As, E Ebrahimie, M Pashaiasl
    Cells, 2021-09-28;10(10):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  28. NGF Eye Administration Recovers the TrkB and Glutamate/GABA Marker Deficit in the Adult Visual Cortex Following Optic Nerve Crush
    Authors: P Rosso, E Fico, LA Mesentier-, V Triaca, A Lambiase, P Rama, P Tirassa
    International Journal of Molecular Sciences, 2021-09-16;22(18):.
    Species: Rat
    Sample Types: Tissue Homogenates
  29. Inhibition of CSF1R, a receptor involved in microglia viability, alters behavioral and molecular changes induced by cocaine
    Authors: MCM da Silva, GF Gomes, H de Barros, AM da Silva, AL Teixeira, FA Moreira, AS de Miranda, ACP de Oliveir
    Scientific Reports, 2021-08-06;11(1):15989.
    Species: Mouse
    Sample Types: Tissue Homogenates
  30. C-terminal domain small phosphatase 1 (CTDSP1) regulates growth factor expression and axonal regeneration in peripheral nerve tissue
    Authors: NM Gervasi, A Dimtchev, DM Clark, M Dingle, AV Pisarchik, LJ Nesti
    Scientific Reports, 2021-07-14;11(1):14462.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. A Single Session of Whole-Body Electromyostimulation Increases Muscle Strength, Endurance and proNGF in Early Parkinson Patients
    Authors: G Fiorilli, F Quinzi, A Buonsenso, G Casazza, L Manni, A Parisi, A Di Costanz, G Calcagno, M Soligo, A di Cagno
    International Journal of Environmental Research and Public Health, 2021-05-20;18(10):.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. VEGF Mediates Retinal M�ller Cell Viability and Neuroprotection through BDNF in Diabetes
    Authors: YZ Le, B Xu, AJ Chucair-El, H Zhang, M Zhu
    Biomolecules, 2021-05-10;11(5):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  33. Peripheral Nerve-Derived Stem Cell Spheroids Induce Functional Recovery and Repair after Spinal Cord Injury in Rodents
    Authors: HL Lee, CE Yeum, H Lee, J Oh, JT Kim, WJ Lee, Y Ha, YI Yang, KN Kim
    International Journal of Molecular Sciences, 2021-04-16;22(8):.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. Influence of Previous COVID-19 and Mastitis Infections on the Secretion of Brain-Derived Neurotrophic Factor and Nerve Growth Factor in Human Milk
    Authors: V Demers-Mat, DJ Hines, RM Hines, S Lavangnana, S Fels, E Medo
    International Journal of Molecular Sciences, 2021-04-08;22(8):.
    Species: Human
    Sample Types: Milk Supernates
  35. Evaluation of the Effect of Hypericum triquetrifolium Turra on Memory Impairment Induced by Chronic Psychosocial Stress in Rats: Role of BDNF
    Authors: KH Alzoubi, L Abdel-Hafi, OF Khabour, T El-Elimat, MA Alzubi, FQ Alali
    Drug Design, Development and Therapy, 2020-12-01;14(0):5299-5314.
    Species: Rat
    Sample Types: Tissue Homogenates
  36. The Effect of Long-Term Aroniamelanocarpa Extract Supplementation on Cognitive Performance, Mood, and Vascular Function: A Randomized Controlled Trial in Healthy, Middle-Aged Individuals
    Authors: S Ahles, YR Stevens, PJ Joris, D Vauzour, J Adam, E de Groot, J Plat
    Nutrients, 2020-08-17;12(8):.
    Species: Human
    Sample Types: Serum
  37. Effects of Regular Taekwondo Intervention on Oxidative Stress Biomarkers and Myokines in Overweight and Obese Adolescents
    Authors: HT Roh, SY Cho, WY So
    Int J Environ Res Public Health, 2020-04-06;17(7):.
    Species: Human
    Sample Types: Plasma
  38. Neuroinflammatory Markers in the Serum of Prepubertal Children with Down Syndrome
    Authors: L Tarani, V Carito, G Ferraguti, C Petrella, A Greco, M Ralli, MP Messina, D Rasio, E De Luca, C Putotto, P Versacci, M Ceccanti, M Fiore
    J Immunol Res, 2020-03-23;2020(0):6937154.
    Species: Human
    Sample Types: Serum
  39. A Cross-Sectional Study Evaluating the Effects of Resistance Exercise on Inflammation and Neurotrophic Factors in Elderly Women with Obesity
    Authors: HT Roh, SY Cho, WY So
    J Clin Med, 2020-03-20;9(3):.
    Species: Human
    Sample Types: Serum
  40. Mast Cells Populate the Corneoscleral Limbus: New Insights for Our Understanding of Limbal Microenvironment
    Authors: A Micera, K Jirsova, G Esposito, BO Balzamino, A Di Zazzo, S Bonini
    Invest. Ophthalmol. Vis. Sci., 2020-03-09;61(3):43.
    Species: Human
    Sample Types: Cell Culture Supernates
  41. The importance of physical fitness for the relationship of BDNF with obesity measures in young normal-weight adults
    Authors: MA Alomari, OF Khabour, K Alawneh, KH Alzoubi, AB Maikano
    Heliyon, 2020-03-03;6(3):e03490.
    Species: Human
    Sample Types: Serum
  42. Chronic Systemic Inflammation Exacerbates Neurotoxicity in a Parkinson's Disease Model
    Authors: P Ugalde-Muñ, I Fetter-Pru, L Navarro, E García, A Chavarría
    Oxid Med Cell Longev, 2020-01-13;2020(0):4807179.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  43. The neuroprotective activity of heat-treated human platelet lysate biomaterials manufactured from outdated pathogen-reduced (amotosalen/UVA) platelet concentrates
    Authors: O Nebie, D Devos, V Vingtdeux, L Barro, JC Devedjian, A Jonneaux, ML Chou, R Bordet, L Buée, F Knutson, D Blum, T Burnouf
    J. Biomed. Sci., 2019-10-31;26(1):89.
    Species: Human
    Sample Types: Cell Culture Supernates
  44. Greater Circulating Copper Concentrations and Copper/Zinc Ratios are Associated with Lower Psychological Distress, But Not Cognitive Performance, in a Sample of Australian Older Adults
    Authors: M Mravunac, EA Szymlek-Ga, R M Daly, B R Roberts, M Formica, J Gianoudis, S L O'Connel, C A Nowson, B R Cardoso
    Nutrients, 2019-10-17;11(10):.
    Species: Human
    Sample Types: Serum
  45. Associations between inflammatory and neurological markers with quality of life and well-being in older adults
    Authors: JL Tait, RL Duckham, CM Milte, LC Main, RM Daly
    Exp. Gerontol., 2019-07-16;125(0):110662.
    Species: Human
    Sample Types: Serum
  46. PER3 variable number tandem repeat (VNTR) polymorphism modulates the circadian variation of the descending pain modulatory system in healthy subjects
    Authors: F Carvalho, M Pedrazzoli, A Gasparin, F Dos Santos, M Zortea, A Souza, I da Silva L, F Fregni, W Caumo
    Sci Rep, 2019-06-27;9(1):9363.
    Species: Human
    Sample Types: Serum
  47. Cerebrospinal fluid BDNF pro-peptide levels in major depressive disorder and schizophrenia
    Authors: T Mizui, K Hattori, S Ishiwata, S Hidese, S Yoshida, H Kunugi, M Kojima
    J Psychiatr Res, 2019-03-26;113(0):190-198.
    Species: Mouse
    Sample Types: CSF
  48. Intracerebral Delivery of Brain-Derived Neurotrophic Factor Using HyStem�-C Hydrogel Implants Improves Functional Recovery and Reduces Neuroinflammation in a Rat Model of Ischemic Stroke
    Authors: K Ravina, DI Briggs, S Kislal, Z Warraich, T Nguyen, RK Lam, TI Zarembinsk, M Shamloo
    Int J Mol Sci, 2018-11-28;19(12):.
    Species: Rat
    Sample Types: Hydrogel Implant
  49. Four types of human platelet lysate, including one virally inactivated by solvent-detergent, can be used to propagate Wharton Jelly mesenchymal stromal cells
    Authors: MS Chen, TJ Wang, HC Lin, B Thierry
    New biotechnology, 2018-11-20;0(0):.
    Species: Human
    Sample Types: Platelet Lysates
  50. Dance training is superior to repetitive physical exercise in inducing brain plasticity in the elderly
    Authors: K Rehfeld, A Lüders, A Hökelmann, V Lessmann, J Kaufmann, T Brigadski, P Müller, NG Müller
    PLoS ONE, 2018-07-11;13(7):e0196636.
    Species: Human
    Sample Types: Serum
  51. Lifelong environmental enrichment in the absence of exercise protects the brain from age-related cognitive decline
    Authors: AM Birch, ÁM Kelly
    Neuropharmacology, 2018-04-07;0(0):.
    Species: Rat
    Sample Types: Tissue Culture Supernates
  52. Does Transcranial Direct Current Stimulation Combined with Peripheral Electrical Stimulation Have an Additive Effect in the Control of Hip Joint Osteonecrosis Pain Associated with Sickle Cell Disease? A Protocol for a One-Session Double Blind, Block-Randomized Clinical Trial
    Authors: TDS Lopes, WDS Silva, SB Ribeiro, CA Figueiredo, FQ Campbell, GC Daltro, A Valenzuela, P Montoya, RCS Lucena, AF Baptista
    Front Hum Neurosci, 2017-12-20;11(0):633.
    Species: Human
    Sample Types: Serum
  53. Association between serum levels of glutamate and neurotrophic factors and response to clozapine treatment
    Authors: A Krivoy, E Hochman, KV Sendt, S Hollander, Y Vilner, M Selakovic, A Weizman, M Taler
    Schizophr. Res., 2017-06-07;0(0):.
    Species: Human
    Sample Types: Serum
  54. Effect of 2-octylcyanoacrylate on placenta derived mesenchymal stromal cells on extracellular matrix
    Authors: YJ Chen, L Lankford, S Kabagambe, Z Saenz, P Kumar, D Farmer, A Wang
    Placenta, 2017-04-02;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  55. High Intensity Exercise as a Dishabituating Stimulus Restores Counterregulatory Responses in Recurrently Hypoglycemic Rodents
    Authors: AD McNeilly, JR Gallagher, JT Huang, ML Ashford, RJ McCrimmon
    Diabetes, 2017-03-07;0(0):.
    Species: Rat
    Sample Types: Whole Blood
  56. Effects of Ranibizumab and Aflibercept on Human M�ller Cells and Photoreceptors under Stress Conditions
    Authors: W Shen, B Yau, SR Lee, L Zhu, M Yam, MC Gillies
    Int J Mol Sci, 2017-03-01;18(3):.
    Species: Human
    Sample Types: Cell Culture Supernates
  57. Exploring Serum Levels of Brain Derived Neurotrophic Factor and Nerve Growth Factor Across Glaucoma Stages
    Authors: F Oddone, G Roberti, A Micera, A Busanello, S Bonini, L Quaranta, L Agnifili, G Manni
    PLoS ONE, 2017-01-09;12(1):e0168565.
    Species: Human
    Sample Types: Serum
  58. Serum levels of neurotrophic factors in active toxoplasmic retinochoroiditis
    Authors: Cynthia Azeredo Cordeiro
    Braz J Infect Dis, 2016-12-05;0(0):.
    Species: Human
    Sample Types: Serum
  59. Neuroendocrine Inflammatory Responses in Overweight/Obese Infants
    PLoS ONE, 2016-12-01;11(12):e0167593.
    Species: Human
    Sample Types: Plasma
  60. Pharmacologically active microcarriers delivering BDNF within a hydrogel: novel strategy for human bone marrow-derived stem cells neural/neuronal differentiation guidance and therapeutic secretome enhancement
    Authors: Claudia N Montero-Me
    Acta Biomater, 2016-11-16;0(0):.
  61. Chrysin promotes attenuation of depressive-like behavior and hippocampal dysfunction resulting from olfactory bulbectomy in mice
    Authors: Silvana Peterini Boeira
    Chem. Biol. Interact., 2016-11-03;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  62. Myokines in Response to a Tournament Season among Young Tennis Players
    Biomed Res Int, 2016-08-29;2016(0):1460892.
    Species: Human
    Sample Types: Serum
  63. Transplantation of human adipose tissue-derived stem cells delays clinical onset and prolongs life span in ALS mouse model.
    Authors: Kim, Kwang S, Lee, Hong J, An, Jin, Kim, Yun B, Ra, Jung Cha, Lim, Inja, Kim, Seung U
    Cell Transplant, 2013-09-18;23(12):1585-97.
    Species: Human
    Sample Types: Cell Culture Supernates
  64. Derivation of injury-responsive dendritic cells for acute brain targeting and therapeutic protein delivery in the stroke-injured rat.
    Authors: Manley, Nathan C, Caso, Javier R, Works, Melissa, Cutler, Andrew B, Zemlyak, Ilona, Sun, Guohua, Munhoz, Carolina, Chang, Sydney, Sorrells, Shawn F, Ermini, Florian, Decker, Johannes, Bertrand, Anthony, Dinkel, Klaus M, Steinberg, Gary K, Sapolsky, Robert M
    PLoS ONE, 2013-04-16;8(4):e61789.
    Species: Human
    Sample Types: Cell Culture Supernates
  65. Improvement in disability after alemtuzumab treatment of multiple sclerosis is associated with neuroprotective autoimmunity.
    Authors: Jones JL, Anderson JM, Phuah CL, Fox EJ, Selmaj K, Margolin D, Lake SL, Palmer J, Thompson SJ, Wilkins A, Webber DJ, Compston DA, Coles AJ
    Brain, 2010-07-21;133(0):2232-47.
    Species: Human
    Sample Types: Cell Culture Supernates
  66. Serum levels of brain-derived neurotrophic factor correlate with motor impairment in Parkinson's disease.
    Authors: Scalzo P, Kummer A, Bretas TL, Cardoso F, Teixeira AL
    J. Neurol., 2009-10-22;257(4):540-5.
    Species: Human
    Sample Types: Serum
  67. Helminth infections associated with multiple sclerosis induce regulatory B cells.
    Authors: Correale J, Farez M, Razzitte G
    Ann. Neurol., 2008-08-01;64(2):187-99.
    Species: Human
    Sample Types: Cell Culture Supernates
  68. Increased plasma brain-derived neurotrophic factor levels in chronic smokers following unaided smoking cessation.
    Authors: Kim TS, Kim DJ, Lee H, Kim YK
    Neurosci. Lett., 2007-07-04;423(1):53-7.
    Species: Human
    Sample Types: Plasma
  69. Decreased plasma BDNF level in depressive patients.
    Authors: Lee BH, Kim H, Park SH, Kim YK
    J Affect Disord, 2006-12-13;101(1):239-44.
    Species: Human
    Sample Types: Plasma
  70. Relationship of serum brain-derived neurotrophic factor level with other markers of disease severity in patients with atopic dermatitis.
    Authors: Namura K, Hasegawa G, Egawa M, Matsumoto T, Kobayashi R, Yano T, Katoh N, Kishimoto S, Ohta M, Obayashi H, Ose H, Fukui M, Nakamura N, Yoshikawa T
    Clin. Immunol., 2006-12-11;122(2):181-6.
    Species: Human
    Sample Types: Serum
  71. Circulating levels of brain-derived neurotrophic factor correlate with disease severity in the intrinsic type of atopic dermatitis.
    Authors: Raap U, Werfel T, Goltz C, Deneka N, Langer K, Bruder M, Kapp A, Schmid-Ott G, Wedi B
    Allergy, 2006-12-01;61(12):1416-8.
    Species: Human
    Sample Types: Serum
  72. Selected neurotrophins, neuropeptides, and cytokines: developmental trajectory and concentrations in neonatal blood of children with autism or Down syndrome.
    Authors: Nelson PG, Kuddo T, Song EY
    Int. J. Dev. Neurosci., 2005-11-14;24(1):73-80.
    Species: Human
    Sample Types: Complex Sample Type
  73. Neurotrophic factors in relapsing remitting and secondary progressive multiple sclerosis patients during interferon beta therapy.
    Authors: Caggiula M, Batocchi AP, Frisullo G, Angelucci F, Patanella AK, Sancricca C, Nociti V, Tonali PA, Mirabella M
    Clin. Immunol., 2005-11-07;118(1):77-82.
    Species: Human
    Sample Types: Cell Culture Supernates
  74. Maternal poly I:C exposure during pregnancy regulates TNF alpha, BDNF, and NGF expression in neonatal brain and the maternal-fetal unit of the rat.
    Authors: Gilmore JH, Jarskog LF, Vadlamudi S
    J. Neuroimmunol., 2004-11-24;159(1):106-12.
    Species: Rat
    Sample Types: Tissue Homogenates
  75. Intracerebral transplantation of carotid body in rats with transient middle cerebral artery occlusion.
    Authors: Yu G, Xu L, Hadman M, Hess DC, Borlongan CV
    Brain Res., 2004-07-23;1015(1):50-6.
    Species: Rat
    Sample Types: Tissue Homogenates

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Human/Mouse BDNF DuoSet ELISA
By Guan-Hong Liou on 03/14/2023
Sample Tested: Serum
Human/Mouse BDNF DuoSet ELISA
By Anonymous on 10/24/2019
Sample Tested: Serum and Plasma

Used both in serum and plasma, best results in Serum diluted 1:100 in Reagent Diluent.

Human/Mouse BDNF DuoSet ELISA DY248