Human MMP-8 Antibody Summary
Phe21-Gly467
Accession # AAZ38714
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human MMP‑8 Monoclonal Antibody (Catalog # MAB908).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Total MMP-8 DuoSet ELISA Kit (Catalog # DY908) for convenient development of a sandwich ELISA or the Human Total MMP-8 Quantikine ELISA Kit (Catalog # DMP800) for a complete optimized ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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MMP‑8 in Human Breast Cancer Tissue. MMP-8 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 8 µg/mL Mouse Anti-Human MMP-8 Monoclonal Antibody (Catalog # MAB9081) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to epithelial cells in terminal ductules (round) and intralobular duct (elongated) composing glandular lobules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Human MMP-8 ELISA Standard Curve. Recombinant Human MMP‑8 protein was serially diluted 2-fold and captured by Mouse Anti-Human MMP‑8 Monoclonal Antibody (MAB908) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human MMP‑8 Monoclonal Antibody (Catalog # MAB9081) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).
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Detection of MMP‑8 in Jurkat cells by Flow Cytometry Jurkat cells were stained with Mouse Anti-Human MMP‑8 Monoclonal Antibody (Catalog # MAB9081, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-8
Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-8 (neutrophil collagenase) is expressed in neutrophils, where it is stored in specific granules. MMP-8 release from the neutrophils is stimulated by various factors such as interleukins 1 and 8, TNF-alpha and GM-CSF. MMP-8 is capable of cleaving types I, II and III triple-helical collagen, gelatin peptides, fibronectin, proteoglycans, aggrecan, serpins, beta -casein and peptides such as angiotensin and substance P. In addition to its function in phagocytosis,
MMP‑8 has a high capacity for infiltrating connective tissue, and is implicated in the breakdown of the extracellular matrix in diseases such as rheumatoid arthritis. Structurally, MMP-8 consists of several domains: a pro-domain that is cleaved upon activation, a catalytic domain containing the zinc-binding site, a short hinge region and a hemopexin-like domain. MMP-8 is heavily glycosylated.
Product Datasheets
Citations for Human MMP-8 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Systematic Evaluation of the Cellular Innate Immune Response During the Process of Human Atherosclerosis.
Authors: van Dijk R A, Rijs K et al.
J Am Heart Assoc
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Collagen degradation and neutrophilic infiltration: a vicious circle in inflammatory bowel disease
Authors: Pim J Koelink, Saskia A Overbeek, Saskia Braber, Mary E Morgan, Paul A J Henricks, Mojtaba Abdul Abdul Roda et al.
Gut
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Matrix metalloproteinase-8 is expressed in human chorion during labor.
Authors: Arechavaleta-Velasco F, Marciano D, Diaz-Cueto L, Parry S
Am. J. Obstet. Gynecol., 2004-03-01;190(3):843-50.
Species: Human
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-P, Western Blot
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