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Human MMP-2 Antibody

Catalog #: MAB9022
3 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB9022
MAB9022-SP
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (2)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MMP-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects the pro and active forms of human MMP-2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human MMP-1, -3, -7, -8, -9, -10, -12, or -13 is observed.
Source
Monoclonal Mouse IgG2B Clone # 101724
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP‑2
Ala30-Cys660
Accession # P08253
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human MMP‑2 Western Blot Standard (Catalog # WBC025)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human MMP‑2 (Catalog # 902-MP), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-2

MMP-2, also called gelatinase A, is a matrix metalloproteinase that can degrade a broad range of substrates including type IV, V, VII and X collagens as well as elastin and fibronectin. MMP-2 is produced by neutrophils, macrophages and monocytes.

Long Name
Matrix Metalloproteinase 2
Entrez Gene IDs
4313 (Human); 17390 (Mouse)
Alternate Names
72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3.4.24; EC 3.4.24.24; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP2; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)

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Citations for Human MMP-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. JNK and p38 Inhibitors Prevent Transforming Growth Factor-&beta1-Induced Myofibroblast Transdifferentiation in Human Graves' Orbital Fibroblasts
    Authors: TY Hou, SB Wu, HC Kau, CC Tsai
    International Journal of Molecular Sciences, 2021-03-14;22(6):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Matrix metalloproteinase activity in pediatric acute lung injury.
    Authors: Kong MY, Gaggar A, Li Y
    Int J Med Sci, 2008-12-16;6(1):9-17.
    Species: Human
    Sample Types: Tissue Secretion
    Applications: Western Blot
  3. Control of matrix metalloproteinase production in human intestinal fibroblasts by interleukin 21.
    Authors: Monteleone G, Caruso R, Fina D, Peluso I, Gioia V, Stolfi C, Fantini MC, Caprioli F, Tersigni R, Alessandroni L, MacDonald TT, Pallone F
    Gut, 2006-05-08;55(12):1774-80.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Reviews for Human MMP-2 Antibody

Average Rating: 5 (Based on 2 Reviews)

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Human MMP-2 Antibody
By Anonymous on 07/23/2018
Application: WB Sample Tested: HepG2 human hepatocellular carcinoma cell line,MDA-MB-231 human breast cancer cell line Species: Human

Total cell lysates from HepG2 and MDA-MB-231 were subjected to western blot. PVDF membrane were probed with 1 um/ml Human MMP-2 Antibody (MAB9022). A specific band was detected for MMP2 at approximately 70 kDa. This experiment was conducted under reducing conditions

Western Blot Human MMP-2 Antibody MAB9022
Human MMP‑2 Antibody
By Anonymous on 07/02/2018
Application: WB Sample Tested: Purified Human MMP2,Purified Human MMP9,Purified MMP9 Species: Human

Does not cross-react with human MMP9