Human MICA/B Alexa Fluor® 700-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB13001N-100
FAB13001N-025
Detection of MICA/B in K562 Human Cell Line by Flow Cytometry.
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Product Details
Citations (1)
FAQs
Reviews (1)

Human MICA/B Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human MICA/B in direct ELISAs.
Source
Monoclonal Mouse IgG2a Clone # 159207
Formulation
Supplied in a saline solution containing BSA and Sodium Azide. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of MICA/B antibody in K562 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of MICA/B in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICA/B Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB13001N, filled histogram) or isotype control antibody (Catalog # IC003N, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Reconstitution
For liquid material, refer to CoA for concentration.
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Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MICA/B

MICA and MICB are stress-induced type I transmembrane proteins that function as ligands for NKG2D. They are distantly related to MHC class I proteins and are expressed on epithelial tumors and in response to infections.

Long Name
MHC Class I-related Protein A and B
Entrez Gene IDs
4276 (Human)
Alternate Names
MHC class I chain-related protein A; MHC class I chain-related protein B; MIC; MICA; MICA/B; MICB; PERB11; PERB11.1; PERB11.2

Product Datasheets

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citation for Human MICA/B Alexa Fluor® 700-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The NK�cell receptor NKp46 recognizes ecto-calreticulin on ER-stressed cells
    Authors: S Sen Santar, DJ Lee, Â Crespo, JJ Hu, C Walker, X Ma, Y Zhang, S Chowdhury, KF Meza-Sosa, M Lewandrows, H Zhang, M Rowe, A McClelland, H Wu, C Junqueira, J Lieberman
    Nature, 2023-04-05;616(7956):348-356.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Human MICA/B Alexa Fluor® 700-conjugated Antibody
By Stephane Hua on 07/05/2018
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human