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Human mGluR2 Antibody

Catalog #: MAB4676 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4676
MAB4676-SP
Detection of mGluR2 in U‑118‑MG Human Cell Line by Flow Cytometry.
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Human mGluR2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human mGluR2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with the N-terminal regions of recombinant human mGluR1, -3, -4, -5, -7, or -8 is observed.
Source
Monoclonal Mouse IgG2B Clone # 455310
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human mGluR2
Glu19-Ser498
Accession # Q14416
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human mGluR2
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of mGluR2 antibody in U-118-MG Human Cell Line antibody by Flow Cytometry. View Larger

Detection of mGluR2 in U‑118‑MG Human Cell Line by Flow Cytometry. U-118-MG human glioblastoma/ astrocytoma cell line was stained with Mouse Anti-Human mGluR2 Monoclonal Antibody (Catalog # MAB4676, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permea-bilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: mGluR2

Metabotropic glutamate receptor 2 (mGluR2) is an 872 aa, predicted 96 kDa multipass G protein coupled inhibitory receptor belonging to group II of the metabotropic glutamate receptor family. It is localized largely on the presynaptic side of glutamatergic and other neurotransmitter synapses in areas of the forebrain. mGluR2 activity is potentially involved in some anxiety disorders. The long N‑terminal extracellular region of human mGluR2 (aa 1‑498) shares 97% aa identity with either mouse or rat mGluR2.

Long Name
Metabotropic Glutamate Receptor 2
Entrez Gene IDs
2912 (Human); 108068 (Mouse); 24415 (Rat)
Alternate Names
GLUR2; glutamate metabotropic receptor 2; glutamate receptor, metabotropic 2; GPRC1B; GPRC1Bmetabotropic glutamate receptor 2; GRM2; mGlu2; mGluR2; MGLUR2glutamate receptor homolog; mGluR7

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