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Human MFAP3 Antibody

Catalog #: MAB10798 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB10798-100
MAB10798-SP
Detection of MFAP3 in Human Tonsil.
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Human MFAP3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human MFAP3 in direct ELISA.
Source
Monoclonal Mouse IgG1 Clone # 1059216
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell, HEK293-derived human MFAP3
Leu22-Met147
Accession # P55082
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
Immersion fixed paraffin-embedded sections of Human Tonsil

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

Detection of MFAP3 in Human Tonsil. MFAP3 was detected in immersion fixed paraffin-embedded sections of Human Tonsil using Mouse Anti-Human MFAP3 Monoclonal Antibody (Catalog # MAB10798) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MFAP3

Microfibril-associated glycoprotein 3 (MFAP3), is a member of the MFAP family which play a role in microfibril assembly, elastinogenesis, and tissue homeostasis. The family members were identified as potential components of fibrillin-containing microfibrils, but they do not share structural similarities (1). MFAP3 is found in ocular zonules. Human MFAP3 is a serine-rich acidic protein located in zonular fibers and consists of an extracellular domain (ECD) containing one Ig-like C2-type domain, a single transmembrane domain, and a cytoplasmic domain. Within the ECD, mature human MFAP3 shares 74% amino acid sequence identity with mouse and rat MFAP3. Among MFAP3-related pathways are degradation of the extracellular matrix and elastic fiber formation. MFAP is a candidate gene for heritable diseases affecting microfibrils (2).

References
  1. Gibson, M.A. Landes Bioscience https://www.ncbi.nlm.nih.gov/books/NBK6448/.
  2. Abrams W.R. (1995) Genomics 26:47.
Long Name
Microfibrillar-Associated Protein 3
Entrez Gene IDs
4238 (Human); 216760 (Mouse); 287299 (Rat)
Alternate Names
DKFZp586F0219; MFAP3; microfibril-associated glycoprotein 3; microfibrillar-associated protein 3

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