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Human MEPE/OF45 Antibody

Catalog #: AF3140 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3140
AF3140-SP
Cell Adhesion Mediated by MEPE and Neutralization by Human MEPE Antibody.
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Human MEPE/OF45 Antibody Summary

Species Reactivity
Human
Specificity
Detects human MEPE/OF45 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MEPE/OF45
Pro18-Asp525
Accession # Q9NQ76
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human MEPE (Catalog # 3140-ME)
Neutralization
Measured by its ability to neutralize MEPE-mediated adhesion of the ATDC5 mouse chondrogenic cell line. The Neutralization Dose (ND50) is typically 2-6 µg/mL in the presence of 2 µg/mL Recombinant Human MEPE.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Adhesion Mediated by MEPE and Neutralization by Human MEPE Antibody. View Larger

Cell Adhesion Mediated by MEPE and Neutralization by Human MEPE Antibody. Recombinant Human MEPE (Catalog # 3140-ME), immobil-ized onto a microplate, supports the adhesion of the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human MEPE (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human MEPE Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF3140). The ND50 is typically 2-6 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MEPE/OF45

MEPE (matrix extracellular phosphoglycoprotein), known as OF45 in mouse and rat, is a 55 kDa member of the SIBLING protein family. MEPE is primarily expressed in bone and dentin, where it regulates the mineralization of those tissues (1‑3). The human MEPE cDNA encodes a 525 amino acid (aa) precursor that includes a 17 aa signal sequence. MEPE contains multiple consensus sites for post-translational modifications, including N-linked glycosylation, N-myristoylation, glycosaminoglycan attachment, and phosphorylation by a variety of kinases. MEPE also contains several putative proteolytic cleavage sites and one integrin-binding RGD motif (3, 4). There is therefore considerable potential for post-translational regulation of MEPE function and its degradation products. MEPE is secreted by osteoblasts and dental pulp stem cells during the mineralization process (5‑7) and also by nonmineralizing tissues including epithelial cells in the renal proximal tubule and salivary duct (8, 9). MEPE has an inhibitory function in bone formation, (5) although a peptide corresponding to aa 242‑264 stimulates new bone formation and the proliferation of osteoblasts and dental pulp stem cells (10, 11). MEPE contains one C‑terminal ASARM motif common to SIBLING proteins. Similar to intact MEPE, the ASARM peptide inhibits bone mineralization and plays a central role in the phosphaturia and reduced mineralization of X-linked hypophosphatemic rickets (HYP) and tumor‑induced osteomalacia (TIO) (12, 13). The zinc metalloprotease Phex binds directly to MEPE via the ASARM motif and prevents ASARM cleavage (13, 14). Multiple inactivating mutations in Phex are found in HYP and TIO and result in the increased liberation of ASARM peptide (15). Both MEPE and ASARM peptide are elevated in these disorders of mineralization and phosphate metabolism (12).

References
  1. Fisher, L.W. and N.S. Fedarko (2003) Connect. Tiss. Res. 44:33.
  2. Quarles, L.D. (2003) Am. J. Physiol. 285:E1.
  3. Qin, C. et al. (2004) Crit. Rev. Oral Biol. Med. 15:126.
  4. Rowe, P.S.N. et al. (2000) Genomics 67:54.
  5. Gowen, L.C. et al. (2003) J. Biol. Chem. 278:1998.
  6. Siggelkow, H. et al. (2004) Bone 35:570.
  7. Liu, H. et al. (2005) Arch. Oral Biol. 50:923.
  8. Ogbureke, K.U.E. and Fisher, L.W. (2005) Kidney Int. 68:155.
  9. Ogbureke, K.U.E. and L.W. Fisher (2004) J. Dent. Res. 83:664.
  10. Hayashibara, T. et al. (2004) J. Bone Miner. Res. 19:455.
  11. Liu, H. et al. (2004) J. Dent. Res. 83:496.
  12. Bresler, D. et al. (2004) J. Endocrinol. 183:R1.
  13. Rowe, P.S.N. et al. (2005) Bone 36:33.
  14. Guo, R. et al. (2002) Biochem. Biophys. Res. Commun. 297:38.
  15. Rowe, P.S. (2004) Crit. Rev. Oral Biol. Med.15:264.
Long Name
Matrix Extracellular Phosphoglycoprotein with ASARM Motif
Entrez Gene IDs
56955 (Human); 94111 (Mouse); 79110 (Rat)
Alternate Names
extracellular phosphoglycoprotein with ASARM motif (bone); matrix extracellular phosphoglycoprotein; MEPE; OF45; Osteoregulin

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