Home / Melanocortin-5 R/MC5R / Human Melanocortin-5 R/MC5R Antibody

Human Melanocortin-5 R/MC5R Antibody

Catalog #: MAB8205
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB8205
MAB8205-SP
Detection of Melanocortin-5 R/MC5R in HEK293 Human Cell Line Transfected with Human Melanocortin-5 R/MC5R and eGFP by Flow Cytometry.
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Human Melanocortin-5 R/MC5R Antibody Summary

Specificity
Stains human Melanocortin-5 R/MC5R transfected cells but not irrelevant transfectants in flow cytometry.
Source
Monoclonal Mouse IgG2A Clone # 821012
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Melanocortin-5 R/MC5R
Met1-Asp325
Accession # P33032
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Melanocortin-5 R/MC5R antibody in HEK293 Human Cell Line Transfected with Human Melanocortin-5 R/MC5R and eGFP antibody by Flow Cytometry. View Larger

Detection of Melanocortin-5 R/MC5R in HEK293 Human Cell Line Transfected with Human Melanocortin-5 R/MC5R and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Melanocortin-5 R/MC5R and eGFP was stained with and either (A) Mouse Anti-Human Melanocortin-5 R/MC5R Monoclonal Antibody (Catalog # MAB8205) or (B) Mouse IgG2AIsotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Melanocortin-5 R/MC5R

Melanocortin 5R (MC5R) is a member of the melanocortin receptor family. The 325 amino acid G-protein-coupled 7-transmembrane glycoprotein is expressed in skeletal muscle, where engagement by a-melanocyte stimulating hormone increases glucose uptake and stimulates a thermogenic response. Expression is also found in sebaceous glands and other skin structures, and on selected hematopoietic cell lines. Human MC5R shares 80-81% amino acid sequence identity with mouse and rat MC5R.

Long Name
Melanocortin 5 Receptor
Entrez Gene IDs
4161 (Human); 17203 (Mouse); 25726 (Rat)
Alternate Names
MC2; MC-2; MC5; MC5R; MC5-R; melanocortin 5 receptor; melanocortin receptor 5; Melanocortin-5 R; melanocortin-5 receptor; Melanocortin5R; Melanocortin-5R

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Citation for Human Melanocortin-5 R/MC5R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Simultaneous Activation of Induced Heterodimerization between CXCR4 Chemokine Receptor and Cannabinoid Receptor 2 (CB2) Reveals a Mechanism for Regulation of Tumor Progression
    Authors: CJ Coke, KA Scarlett, MA Chetram, KJ Jones, BJ Sandifer, AS Davis, AI Marcus, CV Hinton
    J. Biol. Chem., 2016-02-03;291(19):9991-10005.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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