Human Lymphotoxin beta R/TNFRSF3 PE-conjugated Antibody
Human Lymphotoxin beta R/TNFRSF3 PE-conjugated Antibody Summary
Gln31-Met227
Accession # P36941
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Lymphotoxin beta R/TNFRSF3 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human Lymphotoxin beta R/TNFRSF3 PE-conjugated Monoclonal Antibody (Catalog # FAB629P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Detection of Human Lymphotoxin beta R/TNFRSF3 by Flow Cytometry Expression of LT beta R and HVEM receptors on BEAS-2B cells.BEAS-2B cells and THP-1 cells (positive control) were treated with anti-PE labeled LT beta R antibody and anti-FITC labeled HVEM antibody. The fluorescence intensity was measured with a flow cytometer. THP-1 cells, a human monocytic cell line, were used as the positive control because they express both LT beta R and HVEM on their cell surface. The fluorescence intensities of LT beta R (A) and HVEM (B) suggest that BEAS-2B cells express LT beta R but not HVEM. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25501580), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Lymphotoxin beta R/TNFRSF3
Lymphotoxin beta Receptor (LT beta R), also known as TNF RIII and TNF R-Related Protein (TNF Rrp) is a member of the TNF receptor superfamily, designated TNFRSF3. Human LT beta R cDNA encodes a 435 amino acid (aa) residue type I membrane protein with a putative 30 aa residue signal peptide, a 193 aa residue extracellular domain and a 171 aa residue cytoplasmic domain. The extracellular domain of LT beta R contains four cysteine-rich motifs characteristic of the TNF receptor superfamily. The cytoplasmic region of LT beta R shares little sequence similarity with other TNF receptor family members, suggesting that different signaling mechanisms may be used. LT beta R is expressed in a variety of tissues including visceral and lymphoid tissues. LT beta R is also expressed by cell lines of monocytic, epithelial, and fibroblastic origins but not by T and B lymphocytes. Human and mouse LT beta R share 76% aa sequence homology. The TNF family ligands that have been shown to bind and activate LT beta R include LIGHT (also a ligand for HVEM) and the heterotrimeric Lymphotoxin LT alpha 1/ beta 2 or LT alpha 2/ beta 1. Depending on the cell type, activation of LT beta R has been shown to induce NF kappa B activation, chemokine production, growth arrest, and apoptosis. In vivo, LT beta R has been shown to play a critical role in controlling cellular immune functions and lymphoid organogenesis.
- Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
- Rennert, P.D. et al. (1998) Immunity 9:71.
- Degli-Esposti, M.A. et al. (1997) J. Immunol 158:1756.
- Mackay, F. et al. (1996) J. Biol. Chem. 271:8618.
- Crowe, P.D. et al. (1994) Science 264:707.
Product Datasheets
Citations for Human Lymphotoxin beta R/TNFRSF3 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Lymphotoxin beta receptor signaling induces IL-8 production in human bronchial epithelial cells.
Authors: Mikami Y, Matsuzaki H, Horie M, Noguchi S, Jo T, Narumoto O, Kohyama T, Takizawa H, Nagase T, Yamauchi Y
PLoS ONE, 2014-12-11;9(12):e114791.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
DcR3 binds to ovarian cancer via heparan sulfate proteoglycans and modulates tumor cells response to platinum with corresponding alteration in the expression of BRCA1.
BMC Cancer, 2012-05-14;12(0):176.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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