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Human Ly6K Antibody

Catalog #: AF6648
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF6648
AF6648-SP
Detection of Human Ly6K by Western Blot.
2 Images
Product Details
Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Ly6K Antibody Summary

Species Reactivity
Human
Specificity
Detects human Ly6K in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Ly6K
Asp18-Gly138
Accession # Q17RY6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Ly6K antibody by Western Blot. View Larger

Detection of Human Ly6K by Western Blot. Western blot shows lysates of human testis tissue. PVDF Membrane was probed with 0.25 µg/mL of Sheep Anti-Human Ly6K Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6648) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Ly6K at approximately 25-27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Ly6K antibody in Human Lung Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Ly6K in Human Lung Cancer Tissue. Ly6K was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Sheep Anti-Human Ly6K Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6648) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ly6K

Ly6K (Lymphocyte antigen 6 locus K; also CO16) is a 26-27 kDa member of the Ly-6 antigen family of molecules. It has restricted expression, being described in testis and skin. Ly6K is found in/on carcinomas, and is known to circulate in normal and tumor-patient blood. Mature human Ly6K is a 121 amino acid (aa) GPI-linked (presumed) glycoprotein. It is synthesized as a 165 aa preproprecursor that contains a 17 aa signal sequence, a 121 aa mature region (aa 18-138), and a 27 aa C‑terminal propeptide. There are two potential splice variants, one that shows a 48 aa substitution, and another that shows a 33 aa substitution for aa 73-165. It is not clear if mouse Ly6K is a true molecular ortholog of human Ly6K. In any event, over aa 18-138, human Ly6K shares 39% aa identity with mouse Ly6K.

Long Name
Lymphocyte Antigen 6 Complex Locus K
Entrez Gene IDs
54742 (Human); 76486 (Mouse)
Alternate Names
cancer/testis antigen 97; CO16; CT97; FLJ35226; HSJ001348; Ly6K; ly-6K; lymphocyte antigen 6 complex, locus K; lymphocyte antigen 6K

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Citations for Human Ly6K Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. LY6K depletion modulates TGF-beta and EGF signaling
    Authors: S Park, D Park, S Han, GE Chung, S Soh, HI Ka, HJ Joo, Y Yang
    Cancer Medicine, 2023-04-19;0(0):.
  2. Small Molecule Binds with Lymphocyte Antigen 6K to Induce Cancer Cell Death
    Authors: S Benti, PB Tiwari, DW Goodlett, L Daneshian, GB Kern, MD Smith, A Uren, M Chruszcz, LS Shimizu, G Upadhyay
    Cancers (Basel), 2020-02-22;12(2):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Ly6E/K Signaling to TGFbeta Promotes Breast Cancer Progression, Immune Escape, and Drug Resistance.
    Authors: AlHossiny M, Luo L, Frazier W, Steiner N, Gusev Y, Kallakury B, Glasgow E, Creswell K, Madhavan S, Kumar R, Upadhyay G
    Cancer Res, 2016-04-11;76(11):3376-86.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  4. Identification of TEX101-associated Proteins Through Proteomic Measurement of Human Spermatozoa Homozygous for the Missense Variant rs35033974
    Authors: Schiza C, Korbakis D, Jarvi K et al.
    Molecular & Cellular Proteomics

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