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Human Lipocalin-2/NGAL Quantikine ELISA Kit

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Human Lipocalin-2/NGAL ELISA Standard Curve
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Summary Sample Values Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human Lipocalin-2/NGAL Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), Heparin Plasma (50 uL), Saliva (50 uL), Urine (50 uL)
Sensitivity
0.04 ng/mL
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma, Saliva, Urine)
Specificity
Natural and recombinant human Lipocalin-2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Sample Values

Serum/Plasma/Saliva/Urine - Samples from apparently healthy volunteers were evaluated for the presence of human Lipocalin-2 in this assay. No medical histories were available for the donors used in this study.

Sample TypeMean (ng/mL)Range (ng/mL)Standard Deviation (ng/mL)
Serum (n=35)11942-17735.6
Heparin plasma (n=35)9436-14928.7
Saliva (n=9)32096-881238
urine (n=19)9.940.40-7217.1

Cell Culture Supernates: 
Human peripheral blood cells (1 x 106 cells/mL) were cultured in DMEM supplemented with 5% fetal bovine serum, 50 μM beta -mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 μg/mL PHA. Aliquots of the cell culture supernates were removed and assayed for levels of human Lipocalin-2.

ConditionDay 1 (ng/mL)Day 5 (ng/mL)
Unstimulated7.737.73
Stimulated14.318.5

A431human epithelial carcinoma cells were grown to confluency in DMEM (high glucose) with 10% fetal bovine serum. An aliquot of the cell culture supernate was removed, assayed for human Lipocalin-2, and measured 130 ng/mL.

Neutrophils were cultured at 1 x 107 cells/mL in Hank's media and stimulated with 50 ng/mL PMA for 30 minutes. An aliquot of the cell culture supernate was removed, assayed for human Lipocalin-2, and measured 699 ng/mL. 

Product Summary

The Quantikine Human Lipocalin-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Lipocalin-2 in cell culture supernates, serum, plasma, urine, and saliva. It contains NS0-expressed recombinant human Lipocalin-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Lipocalin-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Lipocalin-2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Heparin Plasma, Saliva, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 1.14 3.45 7.54 1.05 3.37 6.92
Standard Deviation 0.041 0.107 0.334 0.083 0.204 0.387
CV% 3.6 3.1 4.4 7.9 6.1 5.6

Recovery

The recovery of Lipocalin-2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 93 88-101
Urine (n=4) 90 84-97

Linearity

To assess the linearity of the assay, samples containing high concentrations of Lipocalin-2 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Lipocalin-2/NGAL ELISA Linearity

Scientific Data

N/A Human Lipocalin-2/NGAL ELISA Standard Curve View Larger

Human Lipocalin-2/NGAL ELISA Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Lipocalin-2/NGAL

The Lipocalin family comprises a diverse group of mostly secreted soluble proteins that bind hydrophobic ligands and act as transporters, carrying small molecules to specific cells. Lipocalins are related by possessing an 8-stranded beta-barrel structure. Lipocalin-1, also named tear lipocalin (TL), von Ebners gland protein (VEG) and tear pre-albumin, binds a large number of hydrophobic molecules and exhibits cysteine proteinase inhibitor and endonuclear activities. Lipocalin-2, also known as neutrophil gelatinase-associated lipocalin (NGAL), is a component of granules in neutrophils from tissues that are normally exposed to microorganisms and is upregulated during inflammation. Lipocalin-2 can form homodimers and can heterodimerize with the neutrophil gelatinase MMP-9.

Long Name:
Neutrophil Gelatinase-associated Lipocalin
Entrez Gene IDs:
3934 (Human); 16819 (Mouse); 170496 (Rat); 102137149 (Cynomolgus Monkey)
Alternate Names:
24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin; Uterocalin
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

The conjugate must remain at 2-8 °C during use. Bring all other reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at 2-8 °C for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL cold Conjugate
  6.   Add 200 µL of cold Conjugate to each well. Cover with a new plate sealer, and incubate at 2-8 °C for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Lipocalin-2/NGAL Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

75 Citations: Showing 1 - 10
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  1. Effects of high-intensity intermittent exercise versus moderate-intensity continuous exercise on renal hemodynamics assessed by ultrasound echo
    Authors: Kawakami, S;Yasuno, T;Kawakami, S;Ito, A;Fujimi, K;Matsuda, T;Nakashima, S;Masutani, K;Uehara, Y;Higaki, Y;Michishita, R;
    Physiological reports
    Species: Human
    Sample Types: Urine, Whole Blood
  2. KIM-1, IL-18, and NGAL, in the Machine Learning Prediction of Kidney Injury among Children Undergoing Hematopoietic Stem Cell Transplantation-A Pilot Study
    Authors: Musia?, K;Stojanowski, J;Mi?kiewicz-Bujna, J;Ka?wak, K;Ussowicz, M;
    International journal of molecular sciences
    Species: Human
    Sample Types: Serum
  3. Optimizing Vancomycin Therapy in Critically Ill Children: A Population Pharmacokinetics Study to Inform Vancomycin Area under the Curve Estimation Using Novel Biomarkers
    Authors: Downes, KJ;Zuppa, AF;Sharova, A;Neely, MN;
    Pharmaceutics
    Species: Human
    Sample Types: Plasma, Urine
  4. Interleukin-19 Levels Are Increased in Palmoplantar Pustulosis and Reduced following Apremilast Treatment
    Authors: K Wolk, D Wilsmann-T, K Witte, TC Brembach, C Kromer, S Gerdes, K Ghoreschi, K Reich, R Mössner, R Sabat
    International Journal of Molecular Sciences, 2023-01-09;24(2):.
    Species: Human
    Sample Types: Plasma
  5. Imbalance in Bone Morphogenic Proteins 2 and 7 Is Associated with Renal and Cardiovascular Damage in Chronic Kidney Disease
    Authors: FJ Manzano-Li, M Sanz-Gómez, D González-M, E Vega-Martí, M Gil-Ortega, A Schulz, MÁ Rubio, G Ruiz-Hurta, LM Ruilope, I Aránguez, R Kreutz, MS Fernández-
    International Journal of Molecular Sciences, 2022-12-20;24(1):.
    Species: Rat
    Sample Types: Plasma
  6. The Effect of a Comprehensive Life-Style Intervention Program of Diet and Exercise on Four Bone-Derived Proteins, FGF-23, Osteopontin, NGAL and Sclerostin, in Overweight or Obese Children and Adolescents
    Authors: SI Karampatso, G Paltoglou, SM Genitsarid, P Kassari, E Charmandar
    Nutrients, 2022-09-13;14(18):.
    Species: Human
    Sample Types: Serum
  7. Serum myo-inositol oxygenase levels at hospital discharge predict progression to chronic kidney disease in community-acquired acute kidney injury
    Authors: TJ Kakkanattu, J Kaur, V Nagesh, M Kundu, K Kamboj, P Kaur, J Sethi, HS Kohli, KL Gupta, A Ghosh, V Kumar, AK Yadav, V Jha
    Scientific Reports, 2022-08-02;12(1):13225.
    Species: Human
    Sample Types: Urine
  8. Neutrophil Gelatinase-Associated Lipocalin and Hypertensive Disorders of Pregnancy: A Cohort Study in Twin Pregnancies
    Authors: S Springer, M Franz, K Worda, ME Gorczyca, P Haslinger, C Worda
    Journal of Clinical Medicine, 2022-07-18;11(14):.
    Species: Human
    Sample Types: Serum
  9. The role of LCN2 and LCN2-MMP9 in spondylitis radiographic development: gender and HLA-B27 status differences
    Authors: FWL Tsui, A Lin, I Sari, Z Zhang, KPH Pritzker, HW Tsui, RD Inman
    Arthritis Research & Therapy, 2022-07-08;24(1):164.
    Species: Human
    Sample Types: Serum
  10. The relationship between Lipocalin-2 level and hepatic steatosis in obese patients with NAFLD after bariatric surgery
    Authors: J Chen, S Lei, Y Huang, X Zha, L Gu, D Zhou, J Li, F Liu, N Li, L Du, X Huang, Z Lin, L Bu, S Qu
    Lipids in Health and Disease, 2022-01-16;21(1):10.
    Species: Human
    Sample Types: Serum
  11. Cell stress response impairs de novo NAD+ biosynthesis in the kidney
    Authors: Y Bignon, A Rinaldi, Z Nadour, V Poindessou, I Nemazanyy, O Lenoir, B Fohlen, P Weill-Rayn, A Hertig, A Karras, P Galichon, M Naesens, D Anglicheau, PE Cippà, N Pallet
    JCI Insight, 2022-01-11;7(1):.
    Species: Human
    Sample Types: Urine
  12. Crosstalk between E-Cadherin/beta-Catenin and NF-kappaB Signaling Pathways: The Regulation of Host-Pathogen Interaction during Leptospirosis
    Authors: SH Hsu, LF Chou, CH Hong, MY Chang, CY Tsai, YC Tian, HY Yang, CW Yang
    International Journal of Molecular Sciences, 2021-12-04;22(23):.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Differences in the Biomarker Profile of De Novo Acute Heart Failure versus Decompensation of Chronic Heart Failure
    Authors: S Nawrocka-M, J Biegus, M Hurkacz, M Guzik, M Rosiek-Bie, EA Jankowska, P Ponikowski, R Zymli?ski
    Biomolecules, 2021-11-16;11(11):.
    Species: Human
    Sample Types: Plasma
  14. An Intervention With Michigan-Grown Wheat in Healthy Adult Humans to Determine Effect on Gut Microbiota: Protocol for a Crossover Trial
    Authors: GA Kinney, EN Haddad, LS Garrow, PKW Ng, SS Comstock
    JMIR research protocols, 2021-10-06;10(10):e29046.
    Species: Human
    Sample Types: Stool
  15. The role of leptospiremia and specific immune response in severe leptospirosis
    Authors: U Limothai, N Lumlertgul, P Sirivongra, W Kulvichit, S Tachaboon, J Dinhuzen, W Chaisuriyo, S Peerapornr, C Chirathawo, K Praditporn, S Eiam-Ong, K Tungsanga, N Srisawat
    Scientific Reports, 2021-07-16;11(1):14630.
    Species: Human
    Sample Types: Serum
  16. The Effects of Beverage Intake after Exhaustive Exercise on Organ Damage, Inflammation and Oxidative Stress in Healthy Males.
    Authors: Tominaga T, Ikemura T, Yada K, Kanda K, Sugama K, Ma S, Choi W, Araya M, Huang J, Nakamura N, Suzuki K
    Antioxidants (Basel), 2021-05-28;10(6):.
    Species: Human
    Sample Types: Urine
  17. Serial Lipocalin 2 and Oncostatin M levels reflect inflammation status and treatment response in axial spondyloarthritis
    Authors: FWL Tsui, A Lin, I Sari, Z Zhang, HW Tsui, RD Inman
    Arthritis Research & Therapy, 2021-05-14;23(1):141.
    Species: Human
    Sample Types: Serum
  18. Organophosphate pesticides and progression of chronic kidney disease among children: A prospective cohort study
    Authors: MH Jacobson, Y Wu, M Liu, K Kannan, AJ Li, M Robinson, BA Warady, S Furth, H Trachtman, L Trasande
    Environment international, 2021-05-02;155(0):106597.
    Species: Human
    Sample Types: Urine
  19. The Role of Endothelins, IL-18, and NGAL in Kidney Hypothermic Machine Perfusion
    Authors: K Tejchman, A Nowacki, K Kotfis, E Skwirczyns, M Kotowski, L Zair, M Ostrowski, J Sienko
    Biomedicines, 2021-04-13;9(4):.
    Species: Human
    Sample Types: Plasma
  20. Urine NGAL and KIM-1-Tubular Injury Biomarkers in Long-Term Survivors of Childhood Solid Tumors: A Cross-Sectional Study
    Authors: E Latoch, K Kono?czuk, K Muszy?ska-, K Taranta-Ja, A Wasilewska, E Szymczak, J Trochim, M Krawczuk-R
    Journal of Clinical Medicine, 2021-01-21;10(3):.
    Species: Human
    Sample Types: Urine
  21. Lipocalin 2 as a potential systemic biomarker for central serous chorioretinopathy
    Authors: A Matet, T Jaworski, E Bousquet, J Canonica, C Gobeaux, A Daruich, M Zhao, M Zola, M Meester-Sm, D Mohabati, F Jaisser, S Yzer, F Behar-Cohe
    Sci Rep, 2020-11-19;10(1):20175.
    Species: Human
    Sample Types: Serum
  22. Identification of plaque ruptures using a novel discriminative model comprising biomarkers in patients with acute coronary syndrome
    Authors: H Kook, DH Jang, JH Kim, JY Cho, HJ Joo, SA Cho, JH Park, SJ Hong, CW Yu, DS Lim
    Sci Rep, 2020-11-19;10(1):20228.
    Species: Human
    Sample Types: Serum
  23. Serially assessed bisphenol A and phthalate exposure and association with kidney function in children with chronic kidney disease in the US and Canada: A longitudinal cohort study
    Authors: MH Jacobson, Y Wu, M Liu, TM Attina, M Naidu, R Karthikraj, K Kannan, BA Warady, S Furth, S Vento, H Trachtman, L Trasande
    PLoS Med, 2020-10-14;17(10):e1003384.
    Species: Human
    Sample Types: Urine
  24. Hepatic lipocalin 2 promotes liver fibrosis and portal hypertension
    Authors: J Chen, J Argemi, G Odena, MJ Xu, Y Cai, V Massey, A Parrish, R Vadigepall, J Altamirano, J Cabezas, P Gines, J Caballeria, N Snider, P Sancho-Bru, S Akira, I Rusyn, B Gao, R Bataller
    Scientific Reports, 2020-09-23;10(1):15558.
    Species: Human
    Sample Types: Serum
  25. Cardiac and renal biomarkers in recreational runners following a 21?km treadmill run
    Authors: TJ Yeo, LH Ling, CSP Lam, JPC Chong, OW Liew, ZL Teo, L Gong, AM Richards, MY Chan
    Clin Cardiol, 2020-09-03;0(0):.
    Species: Human
    Sample Types: Serum
  26. The role of specific biomarkers, as predictors of post-operative complications following flexible ureterorenoscopy (FURS), for the treatment of kidney stones: a single-centre observational clinical pilot-study in 37 patients
    Authors: SF Hughes, AJ Moyes, RM Lamb, P Ella-Tongw, C Bell, A Moussa, I Shergill
    BMC Urol, 2020-08-14;20(1):122.
    Species: Human
    Sample Types: Plasma, Serum
  27. Proteomic identification of early urinary-biomarkers of acute kidney injury in preterm infants
    Authors: YH Jung, D Han, SH Shin, EK Kim, HS Kim
    Sci Rep, 2020-03-04;10(1):4057.
    Species: Human
    Sample Types: Urine
  28. Cerebrospinal fluid lipocalin 2 as a novel biomarker for the differential diagnosis of vascular dementia
    Authors: F Llorens, P Hermann, A Villar-Piq, D Diaz-Lucen, K Nägga, O Hansson, I Santana, M Schmitz, C Schmidt, D Varges, S Goebel, J Dumurgier, H Zetterberg, K Blennow, C Paquet, I Baldeiras, I Ferrer, I Zerr
    Nat Commun, 2020-01-30;11(1):619.
    Species: Human
    Sample Types: CSF
  29. Clinical relevance of cell-free mitochondrial DNA during the early postoperative period in kidney transplant recipients
    Authors: K Kim, H Moon, YH Lee, JW Seo, YG Kim, JY Moon, JS Kim, KH Jeong, TW Lee, CG Ihm, SH Lee
    Sci Rep, 2019-12-09;9(1):18607.
    Species: Human
    Sample Types: Urine
  30. Secretory Leukocyte Protease Inhibitor (SLPI)-A Novel Predictive Biomarker of Acute Kidney Injury after Cardiac Surgery: A Prospective Observational Study
    Authors: L Averdunk, C Fitzner, T Levkovich, DE Leaf, M Sobotta, J Vieten, A Ochi, G Moeckel, G Marx, C Stoppe
    J Clin Med, 2019-11-09;8(11):.
    Species: Human
    Sample Types: Urine
  31. Changes in Water Soluble Uremic Toxins and Urinary Acute Kidney Injury Biomarkers After 10- and 100-km Runs
    Authors: W Wo?yniec, K Kasprowicz, J Giebu?towi, N Korytowska, K Zorena, M Bartoszewi, P Rita-Tkach, M Renke, W Ratkowski
    Int J Environ Res Public Health, 2019-10-28;16(21):.
    Species: Human
    Sample Types: Urine
  32. A circulating miRNA signature for early diagnosis of acute kidney injury following acute myocardial infarction
    Authors: PC Fan, CC Chen, CC Peng, CH Chang, CH Yang, C Yang, LJ Chu, YC Chen, CW Yang, YS Chang, PH Chu
    J Transl Med, 2019-04-30;17(1):139.
    Species: Human
    Sample Types: Serum
  33. The role of renal biomarkers to predict the need of surgery in congenital urinary tract obstruction in infants
    Authors: D Kostic, GPNS Beozzo, SB do Couto, AHT Kato, L Lima, P Palmeira, VLJ Krebs, V Bunduki, RPV Francisco, M Zugaib, FT Dénes, WB de Carvalh, VHK Koch
    J Pediatr Urol, 2019-03-16;0(0):.
    Species: Human
    Sample Types: Urine
  34. Polyomavirus BK, BKV microRNA, and urinary neutrophil gelatinase-associated lipocalin can be used as potential biomarkers of lupus nephritis
    Authors: YJ Li, HH Wu, SH Liu, KH Tu, CC Lee, HH Hsu, MY Chang, KH Yu, W Chen, YC Tian
    PLoS ONE, 2019-01-14;14(1):e0210633.
    Species: Human
    Sample Types: Urine
  35. Evaluation of neutrophil gelatinase-associated lipocalin and cystatin C as biomarkers of acute kidney injury after ST-segment elevation myocardial infarction treated by percutaneous coronary intervention
    Authors: LS Nguyen, V Spagnoli, M Kerneis, M Hauguel-Mo, O Barthélémy, JP Collet, G Montalesco, J Silvain
    Arch Cardiovasc Dis, 2019-01-09;0(0):.
    Species: Human
    Sample Types: Plasma
  36. Changes in brain white matter structure are associated with urine proteins in urologic chronic pelvic pain syndrome (UCPPS): A MAPP Network study
    Authors: DC Woodworth, A Dagher, A Curatolo, M Sachdev, C Ashe-McNal, BD Naliboff, JS Labus, JR Landis, JJ Kutch, EA Mayer, RS Lee, MA Moses, BM Ellingson
    PLoS ONE, 2018-12-05;13(12):e0206807.
    Species: Human
    Sample Types: Urine
  37. Preoperative risk assessment improves biomarker detection for predicting acute kidney injury after cardiac surgery
    Authors: CC Lee, CH Chang, SW Chen, PC Fan, SW Chang, YT Chen, YY Nan, PJ Lin, FC Tsai
    PLoS ONE, 2018-09-04;13(9):e0203447.
    Species: Human
    Sample Types: Urine
  38. Hemojuvelin Predicts Acute Kidney Injury and Poor Outcomes Following Cardiac Surgery
    Authors: SW Ko, NH Chi, CH Wu, TM Huang, SJ Chueh, CH Wang, JH Lin, WJ Wang, JT Ting, HM Chang, R Connolly, CH Lai, LJ Tseng, VC Wu, TS Chu
    Sci Rep, 2018-01-31;8(1):1938.
    Species: Human
    Sample Types: Urine
  39. Neutrophil gelatinase-associated lipocalin and acute kidney injury in endovascular aneurysm repair or open aortic repair: a pilot study
    Authors: B Rampoldi, S Tessarolo, P Giubbilini, P Gaia, SD Corino, S Mazza, R Rigolini, MD Poli, E Vianello, MMC Romanelli, E Costa
    Biochem Med (Zagreb), 2018-01-10;28(1):010904.
    Species: Human
    Sample Types: Serum
  40. Prediction of delayed graft function and long-term graft survival by serum and urinary neutrophil gelatinase-associated lipocalin during the early postoperative phase after kidney transplantation
    Authors: HT Maier, MI Ashraf, C Denecke, S Weiss, F Augustin, F Messner, N Vallant, M Böcklein, C Margreiter, G Göbel, J Pratschke, D Öfner-Vela, F Aigner
    PLoS ONE, 2018-01-05;13(1):e0189932.
    Species: Human
    Sample Types: Urine
  41. Real-Time and Non-invasive Monitoring of the Activation of the IRE1?-XBP1 Pathway in Individuals with Hemodynamic Impairment
    Authors: B Fohlen, Q Tavernier, TM Huynh, C Caradeuc, D Le Corre, G Bertho, B Cholley, N Pallet
    EBioMedicine, 2017-12-20;0(0):.
    Species: Human
    Sample Types: Urine
  42. Neutrophil gelatinase associated lipocalin (NGAL) is elevated in type 2 diabetics with carotid artery stenosis and reduced under metformin treatment
    Authors: W Eilenberg, S Stojkovic, A Piechota-P, A Kaider, N Kozakowski, WJ Weninger, J Nanobachvi, J Wojta, I Huk, S Demyanets, C Neumayer
    Cardiovasc Diabetol, 2017-08-08;16(1):98.
    Species: Human
    Sample Types: Serum
  43. Experimental and Human Evidence for Lipocalin-2 (Neutrophil Gelatinase-Associated Lipocalin [NGAL]) in the Development of Cardiac Hypertrophy and heart failure
    Authors: FZ Marques, PR Prestes, SG Byars, SC Ritchie, P Würtz, SK Patel, SA Booth, I Rana, Y Minoda, SP Berzins, CL Curl, JR Bell, B Wai, PM Srivastava, AJ Kangas, P Soininen, S Ruohonen, M Kähönen, T Lehtimäki, E Raitoharju, A Havulinna, M Perola, O Raitakari, V Salomaa, M Ala-Korpel, J Kettunen, M McGlynn, J Kelly, ME Wlodek, PA Lewandowsk, LM Delbridge, LM Burrell, M Inouye, SB Harrap, FJ Charchar
    J Am Heart Assoc, 2017-06-14;6(6):.
    Species: Human
    Sample Types: Plasma
  44. Ascorbic acid ameliorates renal injury in a murine model of contrast-induced nephropathy
    Authors: K Rollins, A Noorani, L Janeckova, T Jones, M Griffiths, MP Baker, JR Boyle
    BMC Nephrol, 2017-03-24;18(1):101.
    Species: Human
    Sample Types: Tissue Homogenates
  45. Follow Up of Value of the Intrarenal Resistivity Indices and Different Renal Biomarkers for Early Identification of Diabetic Nephropathy in Type 1 Diabetic Patients
    Authors: SAE Dayem, AEME Bohy, M Hamed, S Ahmed
    Open Access Maced J Med Sci, 2017-03-20;5(2):188-192.
    Species: Human
    Sample Types: Urine
  46. Lipocalin-2 Promotes Pancreatic Ductal Adenocarcinoma by Regulating Inflammation in the Tumor Microenvironment
    Authors: S Gomez-Chou, A Swidnicka-, N Badi, M Chavez-Tom, GB Lesinski, T Bekaii-Saa, MR Farren, TA Mace, C Schmidt, Y Liu, D Deng, RF Hwang, L Zhou, TT Moore, D Chatterjee, H Wang, X Leng, RB Arlinghaus, CD Logsdon, Z Cruz-Monse
    Cancer Res, 2017-03-01;0(0):.
    Species: Human
    Sample Types: Serum
  47. Altered Systemic Adipokines in Patients with Chronic Urticaria
    Authors: Hoang Kim Tu Trinh
    Int. Arch. Allergy Immunol, 2016-12-01;171(2):102-110.
    Species: Human
    Sample Types: Serum
  48. Perioperative Elevation in Cell-Free DNA Levels in Patients Undergoing Cardiac Surgery: Possible Contribution of Neutrophil Extracellular Traps to Perioperative Renal Dysfunction
    Authors: Koshi Makita
    Anesthesiol Res Pract, 2016-11-02;2016(0):2794364.
    Species: Human
    Sample Types: Serum
  49. Acute Stress Responses Are Early Molecular Events of Retinal Degeneration in Abca4-/-Rdh8-/- Mice After Light Exposure
    Authors: Tanu Parmar
    Invest Ophthalmol Vis Sci, 2016-06-01;57(7):3257-67.
    Species: Human
    Sample Types: Cell Culture Supernates, Cell Lysates
  50. Urinary Neutrophil Gelatinase-Associated Lipocalin (NGAL) in Patients with Obstructive Sleep Apnea
    PLoS ONE, 2016-05-05;11(5):e0154503.
    Species: Human
    Sample Types: Urine
  51. Neonatal acute kidney injury following Valsartan exposure in utero: report of two cases
    Authors: V Drossou-Ag
    Hippokratia, 2016-01-01;20(1):73-75.
    Species: Human
    Sample Types: Urine
  52. Combined Biomarker Analysis for Risk of Acute Kidney Injury in Patients with ST-Segment Elevation Myocardial Infarction.
    Authors: Tung Y, Chang C, Chen Y, Chu P
    PLoS ONE, 2015-04-08;10(4):e0125282.
    Species: Human
    Sample Types: Serum
  53. Lipocalin 2: a new mechanoresponding gene regulating bone homeostasis.
    Authors: Rucci N, Capulli M, Piperni S, Cappariello A, Lau P, Frings-Meuthen P, Heer M, Teti A
    J Bone Miner Res, 2015-02-01;30(2):357-68.
    Species: Human
    Sample Types: Serum
  54. A case-control study of pre-operative levels of serum neutrophil gelatinase-associated lipocalin and other potential inflammatory markers in colorectal cancer.
    Authors: Duvillard L, Ortega-Deballon P, Bourredjem A, Scherrer M, Mantion G, Delhorme J, Deguelte-Lardiere S, Petit J, Bonithon-Kopp C
    BMC Cancer, 2014-12-03;14(0):912.
    Species: Human
    Sample Types: Serum
  55. Growth differentiation factor-15 (GDF-15) levels are associated with cardiac and renal injury in patients undergoing coronary artery bypass grafting with cardiopulmonary bypass.
    Authors: Kahli A, Guenancia C, Zeller M, Grosjean S, Stamboul K, Rochette L, Girard C, Vergely C
    PLoS ONE, 2014-08-29;9(8):e105759.
    Species: Human
    Sample Types: Plasma
  56. Unchanged plasma levels of the soluble urokinase plasminogen activator receptor in elective coronary artery bypass graft surgery patients and cardiopulmonary bypass use.
    Authors: Gozdzik W, Adamik B, Gozdzik A, Rachwalik M, Kustrzycki W, Kubler A
    PLoS ONE, 2014-06-09;9(6):e98923.
    Species: Human
    Sample Types: Plasma
  57. Autophagy activation reduces renal tubular injury induced by urinary proteins.
    Authors: Liu, Wei Jing, Luo, Mian-Na, Tan, Jin, Chen, Wei, Huang, Lei-zhao, Yang, Chen, Pan, Qingjun, Li, Benyi, Liu, Hua-feng
    Autophagy, 2013-11-26;10(2):243-56.
    Species: Human
    Sample Types: Cell Culture Supernates
  58. Urinary L-FABP and its combination with urinary NGAL in early diagnosis of acute kidney injury after cardiac surgery in adult patients.
    Biomarkers, 2012-11-21;18(1):95-101.
    Species: Human
    Sample Types: Urine
  59. Prevalence of Chronic Kidney Disease and Progression of Disease Over Time among Patients Enrolled in the Houston West Nile Virus Cohort.
    Authors: Nolan MS, Podoll AS, Hause AM
    PLoS ONE, 2012-07-06;7(7):e40374.
    Species: Human
    Sample Types: Urine
  60. Lipocalin 2, the TNF-like receptor TWEAKR and its ligand TWEAK act downstream of NFAT1 to regulate breast cancer cell invasion.
    Authors: Gaudineau B, Fougere M, Guaddachi F, Lemoine F, de la Grange P, Jauliac S
    J Cell Sci, 2012-07-05;125(0):4475-86.
    Species: Human
    Sample Types: Cell Culture Supernates
  61. The association between the preoperative serum levels of lipocalin-2 and matrix metalloproteinase-9 (MMP-9) and prognosis of breast cancer.
    BMC Cancer, 2012-05-28;12(0):193.
    Species: Human
    Sample Types: Serum
  62. Indomethacin reduces glomerular and tubular damage markers but not renal inflammation in chronic kidney disease patients: a post-hoc analysis.
    Authors: de Borst MH, Nauta FL, Vogt L, Laverman GD, Gansevoort RT, Navis G
    PLoS ONE, 2012-05-25;7(5):e37957.
    Species: Human
    Sample Types: Urine
  63. Urinary neutrophil gelatinase-associated lipocalin and L-type fatty acid binding protein as diagnostic markers of early acute kidney injury after liver transplantation.
    Authors: Li Y, Zhu M, Xia Q
    Biomarkers, 2012-03-29;17(4):336-42.
    Species: Human
    Sample Types: Urine
  64. Acute kidney injury biomarkers for patients in a coronary care unit: a prospective cohort study.
    Authors: Chen TH, Chang CH, Lin CY, Jenq CC, Chang MY, Tian YC, Hung CC, Fang JT, Yang CW, Wen MS, Lin FC, Chen YC
    PLoS ONE, 2012-02-22;7(2):e32328.
    Species: Human
    Sample Types: Serum
  65. Urinary neutrophil gelatinase-associated lipocalin is a potential biomarker for renal damage in patients with systemic lupus erythematosus.
    Authors: Yang CC, Hsieh SC, Li KJ, Wu CH, Lu MC, Tsai CY, Yu CL
    J. Biomed. Biotechnol., 2012-02-15;2012(0):759313.
    Species: Human
    Sample Types: Urine
  66. Isotretinoin increases skin-surface levels of neutrophil gelatinase-associated lipocalin in patients treated for severe acne.
    Authors: Lumsden KR, Nelson AM, Dispenza MC, Gilliland KL, Cong Z, Zaenglein AL, Thiboutot DM
    Br. J. Dermatol., 2011-08-01;165(2):302-10.
    Species: Human
    Sample Types: Complex Sample Type
  67. Elevated Levels of Neutrophil Gelatinase-Associated Lipocalin in Bile From Patients With Malignant Pancreatobiliary Disease.
    Authors: Zabron AA, Horneffer-van der Sluis VM, Wadsworth CA, Laird F, Gierula M, Thillainayagam AV, Vlavianos P, Westaby D, Taylor-Robinson SD, Edwards RJ, Khan SA
    Am. J. Gastroenterol., 2011-06-14;106(0):1711-7.
    Species: Human
    Sample Types: Bile
  68. Upregulation of lipocalin 2 in adipose tissues of severely obese women: positive relationship with proinflammatory cytokines.
    Authors: Auguet T, Quintero Y, Terra X, Martinez S, Lucas A, Pellitero S, Aguilar C, Hernandez M, Del Castillo D, Richart C
    Obesity (Silver Spring), 2011-03-31;19(12):2295-300.
    Species: Human
    Sample Types: Serum
  69. Urinary excretion of twenty peptides forms an early and accurate diagnostic pattern of acute kidney injury.
    Authors: Metzger J, Kirsch T, Schiffer E, Ulger P, Mentes E, Brand K, Weissinger EM, Haubitz M, Mischak H, Herget-Rosenthal S
    Kidney Int., 2010-09-08;78(12):1252-62.
    Species: Human
    Sample Types: Urine
  70. Circulating levels of matrix metalloproteinase-9 (MMP-9), neutrophil gelatinase-associated lipocalin (NGAL) and their complex MMP-9/NGAL in breast cancer disease.
    Authors: Provatopoulou X, Gounaris A, Kalogera E
    BMC Cancer, 2009-11-04;9(0):390.
    Species: Human
    Sample Types: Serum
  71. Lipocalin 2 regulation by thermal stresses: protective role of Lcn2/NGAL against cold and heat stresses.
    Authors: Roudkenar MH, Halabian R, Roushandeh AM, Nourani MR, Masroori N, Ebrahimi M, Nikogoftar M, Rouhbakhsh M, Bahmani P, Najafabadi AJ, Shokrgozar MA
    Exp. Cell Res., 2009-09-02;315(18):3140-51.
    Species: Human
    Sample Types: Cell Culture Supernates
  72. Lipocalin 2 promotes breast cancer progression.
    Authors: Yang J, Bielenberg DR, Rodig SJ, Doiron R, Clifton MC, Kung AL, Strong RK, Zurakowski D, Moses MA
    Proc. Natl. Acad. Sci. U.S.A., 2009-02-23;106(10):3913-8.
    Species: Human
    Sample Types: Urine
  73. Ex vivo and in vivo regulation of lipocalin-2, a novel adipokine, by insulin.
    Authors: Tan BK, Adya R, Shan X, Syed F, Lewandowski KC, O'Hare JP, Randeva HS
    Diabetes Care, 2008-10-13;32(1):129-31.
    Species: Human
    Sample Types: Cell Culture Supernates
  74. Retinol-binding protein 4 and lipocalin-2 in childhood and adolescent obesity: when children are not just "small adults".
    Authors: Kanaka-Gantenbein C, Margeli A, Pervanidou P, Sakka S, Mastorakos G, Chrousos GP, Papassotiriou I
    Clin. Chem., 2008-05-16;54(7):1176-82.
    Species: Human
    Sample Types: Serum
  75. Serum concentrations of atherogenic proteins neutrophil gelatinase-associated lipocalin and its complex with matrix metalloproteinase-9 are significantly lower in women with polycystic ovary syndrome: hint of a protective mechanism?
    Authors: Diamanti-Kandarakis E, Livadas S, Kandarakis SA, Margeli A, Papassotiriou I
    Eur. J. Endocrinol., 2008-04-01;158(4):525-31.
    Species: Human
    Sample Types: Serum

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Human Lipocalin-2/NGAL Quantikine ELISA Kit
By Andres Ocampo Carrillo on 02/24/2023
Sample Tested: Human retina

The quantification of human lipocalin-2/NGAL was used in retina obtained from a clinical study quantifying diabetic retinopathy. In this model, the disease samples from patients displaying retinopathy were used as those having the pro-inflammatory effect. In the vitreous compartment, lysis of this tissue was used to quantify human lipocalin-2.

Human Lipocalin-2/NGAL Quantikine ELISA Kit DLCN20
Human Lipocalin-2/NGAL Quantikine ELISA Kit
By Anonymous on 10/24/2020
Sample Tested: Urine

Porcine urine samples

Human Lipocalin-2/NGAL Quantikine ELISA Kit DLCN20
Human Lipocalin-2/NGAL Quantikine ELISA Kit
By Stephen Brindley on 04/24/2018
Sample Tested: Urine

Most urine samples fell within the curve at a 1 to 2 dilution. The higher NGAL samples required dilution at 1 to 10

Human Lipocalin-2/NGAL Quantikine ELISA Kit DLCN20
Human Lipocalin-2/NGAL Quantikine ELISA Kit
By Anonymous on 07/18/2017
Sample Tested: Serum

Performed ideally and is probably the best kit for analyzing lipocalin2 out there! Although results should be analyzed at once after completion as saving the ELISA plate over night creates dark spots inside of the wells (see picture/data). Don´t forget to dilute serum!

Human Lipocalin-2/NGAL Quantikine ELISA Kit DLCN20