Human Lipase I Antibody

Catalog #: AF5909 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5909
AF5909-SP

Detection of Human Lipase I by Western Blot.

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Human Lipase I Antibody Summary

Specificity

Detects human Lipase I in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Lipase I
Arg14-His154
Accession # Q6XZB0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Lipase I by Western Blot. Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Lipase I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5909) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Lipase I at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lipase I

Lipase I (Lipase member I; also mPA‑PLA₁ beta  LPDL and PLA1C) is a 55 kDa member of the PLA₁ subfamily, pancreatic lipase family, AB hydrolase superfamily of enzymes. It is expressed by sperm, where it is apparently secreted and subsequently binds to membrane-associated HSPGs. Lipase I hydrolyzes phosphatidic acid to generate 2‑acyl‑LPA, which then activates one of four G-protein coupled receptors. Human Lipase I precursor is 460 amino acids (aa) in length. It contains a 15 aa signal sequence, followed by an active catalytic site composed of Ser159, Asp183 and His268. Lipase I is known to be phosphorylated on Ser25. There are two isoform variants, both of which show a 34 aa substitution for aa 1‑13, and only one of which shows an eight aa substitution for aa 245‑460. Over aa 14‑154, human Lipase I shares 61% aa identity with mouse Lipase I.

Entrez Gene IDs

149998 (Human); 288322 (Rat)

Alternate Names

Cancer/testis antigen 17; CT17; CT17membrane-associated phospholipase A1 beta; EC 3.1.1; EC 3.1.1.-; EC 3.1.1.3; EC 3.1.1.34; Lipase I; lipase, member I; LIPI; LPD lipase; LPDL; LPDLlipase member I; Membrane-associated phosphatidic acid-selective phospholipase A1-beta; mPA-PLA1 beta; PRED5