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Human LIGHT/TNFSF14 PE-conjugated Antibody

Catalog #: FAB664P
5 Citations Datasheet / COA / SDS
Clone 115520 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB664P
Detection of LIGHT/TNFSF14 in Human CD3+T cells by Flow Cytometry.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LIGHT/TNFSF14 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human LIGHT/TNFSF14 in ELISAs. In sandwich immunoassays, no significant cross-reactivity or interference with recombinant human (rh) Fas Ligand, rhAPRIL, recombinant mouse TRANCE, rhTNF-alpha or rhTRAIL is observed.
Source
Monoclonal Mouse IgG1 Clone # 115520
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LIGHT/TNFSF14
Asp74-Val240
Accession # O43557
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of LIGHT/TNFSF14 antibody in Human CD3<SUP>+</SUP>T cells antibody by Flow Cytometry. View Larger

Detection of LIGHT/TNFSF14 in Human CD3+T cells by Flow Cytometry. Human CD3+T cells treated with 1 ng/mL PMA and 1 µg/mL Calcium Ionomycin for 36 hours were stained with Mouse Anti-Human LIGHT/TNFSF14 PE-conjugated Monoclonal Antibody (Catalog # FAB664P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). Cells were gated on CD4+cells using Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: LIGHT/TNFSF14

Human LIGHT, also known as TNFSF14, is a type II membrane protein that is a member of the TNF superfamily. LIGHT is an acronym which stands for "is homologous to lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes". LIGHT has also been called HVEM-L and LT-gamma. LIGHT is a 240 amino acid (aa) protein that contains a 37 aa cytoplasmic domain, a 22 aa transmembrane region, and a 181 aa extracellular domain. Similar to other TNF ligand family members, LIGHT is predicted to assemble as a homotrimer. LIGHT is produced by activated T cells and was first identified by its ability to compete with HSV glycoprotein D for HVEM binding. LIGHT has also been shown to bind to the lymphotoxin beta receptor (LT beta R) and the decoy receptor (DcR3/TR6). LIGHT overexpression in tumor cells induces apoptosis, which can be enhanced by IFN-gamma.

References
  1. Mauri, D.N. et al. (1998) Immunity 8:21.
  2. Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
  3. Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548.
  4. Yu, K-Y. et al. (1999) J. Biol. Chem. 274:13733.
Long Name
TNF Ligand Superfamily Member 14
Entrez Gene IDs
8740 (Human); 50930 (Mouse)
Alternate Names
CD258 antigen; CD258; delta transmembrane LIGHT; Herpes virus entry mediator ligand; Herpesvirus entry mediator ligand; HVEM-L; HVEMLherpesvirus entry mediator-ligand; ligand for herpesvirus entry mediator; LIGHT; LIGHTherpesvirus entry mediator A; LTg; TNFSF14; TR2; tumor necrosis factor (ligand) superfamily, member 14; tumor necrosis factor ligand superfamily member 14; tumor necrosis factor receptor-like 2; tumor necrosis factor superfamily member LIGHT

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Citations for Human LIGHT/TNFSF14 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Shedding "LIGHT" on the Link between Bone and Fat in Obese Children and Adolescents
    Authors: G Brunetti, MF Faienza, L Piacente, G Storlino, A Oranger, G D'Amato, G De Filippo, S Colucci, M Grano
    Int J Mol Sci, 2020-07-03;21(13):.
    Species: Human
    Sample Types: Whole Blood, Whole Cells
    Applications: Cell Culture, Flow Cytometry
  2. Licensed human natural killer cells aid dendritic cell maturation via TNFSF14/LIGHT.
    Authors: Holmes T, Wilson E, Black E, Benest A, Vaz C, Tan B, Tanavde V, Cook G
    Proc Natl Acad Sci U S A, 2014-12-15;111(52):E5688-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. DcR3 binds to ovarian cancer via heparan sulfate proteoglycans and modulates tumor cells response to platinum with corresponding alteration in the expression of BRCA1.
    BMC Cancer, 2012-05-14;12(0):176.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Platelet-associated LIGHT (TNFSF14) mediates adhesion of platelets to human vascular endothelium.
    Authors: Celik S, Langer H, Stellos K, May AE, Shankar V, Kurz K, Katus HA, Gawaz MP, Dengler TJ
    Thromb. Haemost., 2007-10-01;98(4):798-805.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes.
    Authors: Otterdal K, Smith C, Oie E, Pedersen TM, Yndestad A, Stang E, Endresen K, Solum NO, Aukrust P, Damas JK
    Blood, 2006-08-01;108(3):928-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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