Human LIFR alpha PE-conjugated Antibody Summary
Gln45-Ser833
Accession # P42702
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of LIF R alpha in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human LIF Ra PE-conjugated Monoclonal Antibody (Catalog # FAB249P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: LIFR alpha
The activities of the pleiotropic cytokine LIF are mediated through a high-affinity heterodimeric receptor complex consisting of two membrane glycoproteins: an alpha subunit (LIF R alpha, also known as LIF R and CD118) that binds LIF with low affinity and the 130 kDa (gp130) subunit that does not bind LIF by itself, but is required for high-affinity binding of LIF by the complex. The gp130 subunit was first described as the signal transducing subunit of the high-affinity IL-6 receptor complex. Besides LIF, the high-affinity heterodimeric LIF receptor complex has been shown to mediate the activities of oncostatin M (OSM), cardiotrophin-1 and ciliary neurotrophic factor (CNTF).
Human LIF R alpha cDNA encodes a 1097 amino acid (aa) residue precursor type I membrane protein with a 44 aa residue signal peptide, a 789 aa residue extracellular domain, a 26 aa residue transmembrane domain, and a 238 aa residue cytoplasmic domain. LIF R alpha is a member of the cytokine receptor family and has extensive homology to gp130. The extracellular domain of LIF R alpha has two cytokine receptor domains and three fibronectin type III repeats. In mouse, mRNAs encoding a soluble LIF R alpha and lacking transmembrane and intracellular domains, have been isolated. Soluble LIF R alpha has been shown to bind LIF and has LIF antagonistic activity.
- Bazan, J.F. 1990, Proc. Natl. Acad. Sci. USA 87:6934.
- Gearing, D.P. (1994) Guidebook to Cytokines and Their Receptors, Academic Press, p130.
- Pennica D. et al. (1995) J. Biol. Chem. 270:10915.
Product Datasheets
Citations for Human LIFR alpha PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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R-SPONDIN2+ mesenchymal cells form the bud tip progenitor niche during human lung development
Authors: Renee F.C. Hein, Joshua H. Wu, Emily M. Holloway, Tristan Frum, Ansley S. Conchola, Yu-Hwai Tsai et al.
Developmental Cell
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Knockdown of Musashi RNA Binding Proteins Decreases Radioresistance but Enhances Cell Motility and Invasion in Triple-Negative Breast Cancer
Authors: FM Troschel, A Minte, YM Ismail, A Kamal, MS Abdullah, SH Ahmed, M Deffner, B Kemper, L Kiesel, HT Eich, SA Ibrahim, M Götte, B Greve
Int J Mol Sci, 2020-03-21;21(6):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Proteotranscriptomics Reveal Signaling Networks in the Ovarian Cancer Microenvironment
Authors: T Worzfeld, F Finkernage, S Reinartz, A Konzer, T Adhikary, A Nist, T Stiewe, U Wagner, M Looso, J Graumann, R Müller
Mol. Cell Proteomics, 2017-11-15;17(2):270-289.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
A transcriptome-based global map of signaling pathways in the ovarian cancer microenvironment associated with clinical outcome
Genome Biol, 2016-05-23;17(1):108.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Expansive generation of functional airway epithelium from human embryonic stem cells.
Authors: McIntyre B, Alev C, Mechael R, Salci K, Lee J, Fiebig-Comyn A, Guezguez B, Wu Y, Sheng G, Bhatia M
Stem Cells Transl Med, 2013-12-03;3(1):7-17.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Sortilin facilitates signaling of ciliary neurotrophic factor and related helical type 1 cytokines targeting the gp130/leukemia inhibitory factor receptor beta heterodimer.
Authors: Larsen JV, Hansen M, Moller B, Madsen P, Scheller J, Nielsen M, Petersen CM
Mol. Cell. Biol., 2010-06-28;17(0):4175.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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