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Human LIF Biotinylated Antibody

Catalog #: BAF250
4 Citations Datasheet / COA / SDS
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BAF250
Human LIF ELISA Standard Curve.
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Product Details
Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LIF Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human LIF in ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human LIF
Ser23-Phe202
Accession # P15018
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human LIF
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human LIF Monoclonal Antibody (Catalog # MAB2502).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human LIF DuoSet ELISA Kit (Catalog # DY7734-05) for convenient development of a sandwich ELISA.

 

Human LIF Sandwich Immunoassay

Recommended Concentration
Reagent
 

Use in combination with:

Capture Reagent: Human LIF Antibody (Catalog # MAB2502)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA View Larger

Human LIF ELISA Standard Curve. Recombinant Human LIF protein was serially diluted 2-fold and captured by Mouse Anti-Human LIF Monoclonal Antibody (Catalog # MAB2502) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human LIF Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF250) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LIF

LIF is a 36‑67 kDa highly glycosylated polypeptide (1, 2) produced by a variety of cells including T cells (3), monocytes (4), fibroblasts (5), osteoblasts (6) and mast cells (7). Consistent with its many synonyms, LIF exhibits a broad spectrum of effects on both hematopoietic and nonhematopoietic cells. For example, LIF inhibits the differentiation of embryonic stem cells (8), up regulates the synthesis of acute phase proteins in hepatocytes (9), down regulates lipoprotein lipase activity in adipocytes (10), and preferentially induces a cholinergic phenotype in sympathetic neurons (11). The receptor for LIF (LIF R) has been isolated and found to be a 190 kDa type I transmembrane glycoprotein (12). Although this molecule binds LIF, the resultant LIF-LIF R complex is not sufficient to transduce an intracellular signal. This capability is provided by a 130 kDa signal transducing subunit (gp130) that is common to the functional receptors for IL-6, IL-11, CNTF, and Oncostatin M (13, 14). Since gp130 is a ubiquitously expressed membrane protein, the presence of LIF R (membrane-bound or soluble form) ultimately determines the cell’s responsiveness to LIF. Cells known to express LIF R include osteoblasts (6), hepatocytes (15), macrophages (15), neurons (5), and megakaryocytes (16). Human and mouse LIF exhibit 78% sequence homology, and human LIF is biologically active on mouse cells.

References
  1. Van-Vlasselaev, P. et al. (1992) Prog. Growth Factor Res. 4:337.
  2. Gough, N.M. (1992) Growth Factors 7:175.
  3. Anegon, N.M, I. et al. (1991) J. Immunol.147:3973.
  4. Gillett, N.A. et al. (1993) Growth Factors 9:301.
  5. Banner, L. R. and P.H. Patterson (1994) Proc. Natl. Acad. Sci. 91:7109.
  6. Allen, E.H. et al. (1990) J. Cell. Physiol. 145:110.
  7. Lorenzo, J.A. et al. (1994) Clin.Immunol. Immunopathol. 70:260.
  8. Williams, R.L. et al. (1988) Nature 336:684.
  9. Baumann, H. et al. (1989) J. Immunol. 143:1163.
  10. Marshall, M.K. et al. (1994) Endocrinology 135:1412.
  11. Ludham, W.H. et al. (1994) Dev. Biol. 164:5283.
  12. Davis, S. et al. (1993) Science 260:18054.
  13. Gearing, D.P. et al. (1991) EMBO J. 10:28395.
  14. Gearing, D.P. et al. (1991) Science 255:1434.
  15. Hilton, D.J. and N.A. Nicola (1992) J. Biol. Chem. 267:102286.
  16. Hilton, D.J. et al. (1992) Ciba17. Foundation Symposium 167:2278.
Long Name
Leukemia Inhibitory Factor
Entrez Gene IDs
3976 (Human); 16878 (Mouse); 403449 (Canine)
Alternate Names
CDF; D Factor; DIA; differentiation inhibitory activity; differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin; HILDA; HILDAcholinergic differentiation factor; leukemia inhibitory factor (cholinergic differentiation factor); leukemia inhibitory factor; LIF; Melanoma-derived LPL inhibitor; MLPLI

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Citations for Human LIF Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Maternal Leukemia Inhibitory Factor (LIF) Promotes Fetal Neurogenesis via a LIF-ACTH-LIF Signaling Relay Pathway
    Authors: Eriko Simamura, Hiroki Shimada, Nobuaki Higashi, Maimi Uchishiba, Hiroki Otani, Toshihisa Hatta
    Endocrinology
  2. Analysis of the SWI/SNF chromatin-remodeling complex during early heart development and BAF250a repression cardiac gene transcription during P19 cell differentiation.
    Authors: Singh AP, Archer TK.
    Nucleic Acids Res
  3. The reprogrammed host: Chlamydia trachomatis-induced up-regulation of glycoprotein 130 cytokines, transcription factors, and antiapoptotic genes.
    Authors: Hess S, 2019, Rheinheimer C, Tidow F, Bartling G, Kaps C, Lauber J, Buer J, Klos A
    7046, 2001-10-01;44(10):2392-401.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  4. Synthetic signal sequences that enable efficient secretory protein production in the yeast Kluyveromyces marxianus
    Authors: Tohru Yarimizu, Mikiko Nakamura, Hisashi Hoshida, Rinji Akada
    Microbial Cell Factories

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