Human Lgr5/GPR49 Antibody

Catalog #: MAB80781 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB80781-100
MAB80781-SP

Detection of Lgr5/GPR49 in HEK293 Human Cell Line Transfected with Human Lgr5/GPR49 and eGFP by Flow Cytometry.

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All Lgr5/GPR49 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Lgr5/GPR49 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Lgr5/GPR-49 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 750835

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line, CHO derived recombinant human Lgr5/GPR-49
Met1-Ile560
Accession # O75473

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Lgr5/GPR49 in HEK293 Human Cell Line Transfected with Human Lgr5/GPR49 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human Lgr5/GPR49 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB80781) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lgr5/GPR49

GPR49 (G-protein-coupled receptor 49), also called LGR5 (leucine-rich repeat GPR 5) is a seven-transmembrane glycoprotein receptor that negatively regulates of Wnt signaling in the developing intestine. Expression of GPR49 is upregulated in intestinal stem cells and intestinal cancer stem cells and promotes carcinogenesis. GPR49 cDNA encodes 907 amino acids (aa), including a long N-terminal extracellular domain (aa 22-561) with 16 LRR domains. Human GPR49 shares 90% aa sequence identity with mouse and rat GPR45 within aa 22-561.

Long Name

Leucine-rich Repeat Containing G Protein-coupled Receptor 5

Entrez Gene IDs

8549 (Human); 14160 (Mouse); 299802 (Rat); 102120216 (Cynomolgus Monkey)

Alternate Names

FEX; G protein-coupled receptor 49; GPR49; GPR49G-protein coupled receptor HG38; GPR67; G-protein coupled receptor 49; G-protein coupled receptor 67; GRP49; HG38; leucine-rich repeat containing G protein-coupled receptor 5; leucine-rich repeat-containing G protein-coupled receptor 5; leucine-rich repeat-containing G-protein coupled receptor 5; Lgr5; MGC117008; orphan G protein-coupled receptor HG38