Home / Leptin/OB / Human Leptin DuoSet ELISA

Human Leptin DuoSet ELISA

Catalog #: DY398
63 Citations 5 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY398
DY398-05
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Human Leptin / OB ELISA Standard Curve
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Product Details
Procedure
Citations (63)
FAQs
Supplemental Products
Reviews (5)
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human Leptin DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
31.2 - 2,000 pg/mL
Sufficient Materials
For five or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Leptin. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A Human Leptin / OB ELISA Standard Curve View Larger

Human Leptin / OB ELISA Standard Curve

Product Datasheets

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Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Leptin/OB

Leptin is a product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of leptin are obese and diabetic, and have reduced activity, metabolism, and body temperature. Rat leptin shares approximately 96% and 82% sequence identity with the mouse and human protein, respectively. The expression of leptin mRNA is restricted to adipose tissue.

Entrez Gene IDs:
3952 (Human); 16846 (Mouse); 25608 (Rat)
Alternate Names:
FLJ94114; LEP; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Leptin; OB; Obese protein; obese, mouse, homolog of; Obesity factor; OBOBS

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Human Leptin DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

63 Citations: Showing 1 - 10
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  1. The strong inverse association between plasma concentrations of soluble tumor necrosis factor receptors type 1 with adiponectin/leptin ratio in older women
    Authors: Augusto da Costa Teixeira, L;Rocha-Vieira, E;Aparecida Soares, L;Mota de Oliveira, F;Aparecida Oliveira Leopoldino, A;Netto Parentoni, A;Amaral Mendonça, V;Cristina Rodrigues Lacerda, A;
    Cytokine
    Species: Human
    Sample Types: Plasma
  2. Relationships between Circulating Biomarkers and Body Composition Parameters in Patients with Metabolic Syndrome: A Community-Based Study
    Authors: Tarabeih, N;Kalinkovich, A;Ashkenazi, S;Cherny, SS;Shalata, A;Livshits, G;
    International journal of molecular sciences
    Species: Human
    Sample Types: Plasma
  3. Association between biomarkers of redox status and cytokines with different patterns of habitual physical activity in eutrophic and overweight/obese preschoolers: multivariate analysis of a cross-sectional study
    Authors: Viegas, ÂA;Santos, T;Nobre, JNP;Santos, JMD;Silva Lage, VKD;Fernandes, AC;Peixoto, MFD;Morais, RLS;Sartorio, A;Mendonça, VA;Lacerda, ACR;
    BMC public health
    Species: Human
    Sample Types: Plasma
  4. Circadian Variation in Human Milk Hormones and Macronutrients
    Authors: Suwaydi, MA;Lai, CT;Rea, A;Gridneva, Z;Perrella, SL;Wlodek, ME;Geddes, DT;
    Nutrients
    Species: Human
    Sample Types: Breast Milk
  5. Dysregulation of adipokines levels among healthy first-degree relatives of type 2 diabetes patients
    Authors: Purnamasari, D;Simanjuntak, CK;Tricaesario, C;Tahapary, DL;Harbuwono, DS;Yunir, E;
    Heliyon
    Species: Human
    Sample Types: Serum
  6. Effects of an Intervention with Selenium and Coenzyme Q10 on Five Selected Age-Related Biomarkers in Elderly Swedes Low in Selenium: Results That Point to an Anti-Ageing Effect-A Sub-Analysis of a Previous Prospective Double-Blind Placebo-Controlled Randomised Clinical Trial
    Authors: Alehagen, U;Alexander, J;Aaseth, JO;Larsson, A;Svensson, E;Opstad, TB;
    Cells
    Species: Human
    Sample Types: Plasma
  7. Inflammatory biomarkers at different stages of Sarcopenia in older women
    Authors: da Costa Teixeira, LA;Avelar, NCP;Peixoto, MFD;Parentoni, AN;Santos, JMD;Pereira, FSM;Danielewicz, AL;Leopoldino, AAO;Costa, SP;Arrieiro, AN;Soares, LA;da Silva Lage, VK;Prates, ACN;Taiar, R;de Carvalho Bastone, A;Oliveira, VC;Oliveira, MX;Costa, HS;Nobre, JNP;Brant, FP;Duarte, TC;Figueiredo, PHS;Mendonça, VA;Lacerda, ACR;
    Scientific reports
    Species: Human
    Sample Types: Plasma
  8. Hypoxia Preconditioned Serum (HPS) Promotes Proliferation and Chondrogenic Phenotype of Chondrocytes In Vitro
    Authors: Jiang, J;Altammar, J;Cong, X;Ramsauer, L;Steinbacher, V;Dornseifer, U;Schilling, AF;Machens, HG;Moog, P;
    International journal of molecular sciences
    Species: Human
    Sample Types: Serum
  9. Increased plasma lipopolysaccharide-binding protein and altered inflammatory mediators in overweight women suggest a state of subclinical endotoxemia
    Authors: Metz, CN;Xue, X;Chatterjee, PK;Adelson, R;Brines, M;Tracey, KJ;Gregersen, PK;Pavlov, VA;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Plasma
  10. High Glucose Promotes Inflammation and Weakens Placental Defenses against E. coli and S. agalactiae Infection: Protective Role of Insulin and Metformin
    Authors: R Jiménez-Es, D Vargas-Alc, P Flores-Esp, AC Helguera-R, O Villavicen, I Mancilla-H, C Irles, YD Torres-Ram, MY Valdespino, P Velázquez-, R Zamora-Esc, M Islas-Lópe, C Carranco-S, L Díaz, V Zaga-Clave, A Olmos-Orti
    International Journal of Molecular Sciences, 2023-03-09;24(6):.
    Species: Human
    Sample Types: Placental Supernates
  11. Obesity promotes radioresistance through SERPINE1-mediated aggressiveness and DNA repair of triple-negative breast cancer
    Authors: YH Su, YZ Wu, DK Ann, JL Chen, CY Kuo
    Cell Death & Disease, 2023-01-21;14(1):53.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Association between Anti-DENV IgM Serum Prevalence and CD11b Expression by Classical Monocytes in Obesity
    Authors: KB Costa, BCC Garcia, MLB Costa, YG Pena, EAB Figueiredo, MHF Ottoni, JD Santos, V de Oliveir, DB de Oliveir, E Rocha-Viei
    Viruses, 2023-01-14;15(1):.
    Species: Human
    Sample Types: Serum
  13. Adiponectin Is a Contributing Factor of Low Appendicular Lean Mass in Older Community-Dwelling Women: A Cross-Sectional Study
    Authors: LAC Teixeira, JM Dos Santos, AN Parentoni, LP Lima, TC Duarte, FP Brant, CDC Neves, FSM Pereira, NCP Avelar, AL Danielewic, AAO Leopoldino, SP Costa, AN Arrieiro, LA Soares, ACN Prates, JNP Nobre, A de Carvalh, VC de Oliveir, MX Oliveira, PH Scheidt Fi, HS Costa, V Amaral Men, R Taiar, AC Rodrigues
    Journal of Clinical Medicine, 2022-12-02;11(23):.
    Species: Human
    Sample Types: Plasma
  14. Blood orange juice intake modulates plasma and PBMC microRNA expression in overweight and insulin resistance women: impact on MAPK and NFkappaB signaling pathways
    Authors: VC Capetini, BJ Quintanilh, DC de Oliveir, AH Nishioka, LA de Matos, LRP Ferreira, FM Ferreira, GR Sampaio, NMA Hassimotto, FM Lajolo, RA Fock, MM Rogero
    The Journal of nutritional biochemistry, 2022-11-26;0(0):109240.
    Species: Human
    Sample Types: Plasma
  15. Exercise training-induced changes in immunometabolic markers in youth badminton athletes
    Authors: FE Rossi, AJ Maldonado, JM Cholewa, SLG Ribeiro, CA de Araújo, C Figueiredo, T Reichel, K Krüger, FS Lira, LG Minuzzi
    Scientific Reports, 2022-09-15;12(1):15539.
    Species: Human
    Sample Types: Plasma
  16. Association between Leptin (G2548A) and Leptin Receptor (Q223R) Polymorphisms with Plasma Leptin, BMI, Stress, Sleep and Eating Patterns among the Multiethnic Young Malaysian Adult Population from a Healthcare University
    Authors: J Mohanraj, UJA D'Souza, SY Fong, IR Karkada, H Jaiprakash
    Oncogene, 2022-07-21;19(14):.
    Species: Human
    Sample Types: Plasma
  17. Exogenous leptin enhances markers of airway fibrosis in a mouse model of chronic allergic airways disease
    Authors: MD Ihrie, VL McQuade, JT Womble, A Hegde, MS McCravy, CVG Lacuesta, RM Tighe, LG Que, JKL Walker, JL Ingram
    Respiratory Research, 2022-05-24;23(1):131.
    Species: Human
    Sample Types: Serum
  18. Di-(2-ethylhexyl) phthalate substitutes accelerate human adipogenesis through PPARgamma activation and cause oxidative stress and impaired metabolic homeostasis in mature adipocytes
    Authors: A Schaffert, I Karkossa, E Ueberham, R Schlichtin, K Walter, J Arnold, M Blüher, JT Heiker, J Lehmann, M Wabitsch, BI Escher, M von Bergen, K Schubert
    Environment international, 2022-05-06;164(0):107279.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Exercise Training Combined with Calanus Oil Supplementation Improves the Central Cardiodynamic Function in Older Women
    Authors: M Št?pán, K Da?ová, M Matouš, E Krauzová, L Sontáková, M Koc, T Larsen, O Kuda, V Štich, L Rossmeislo, M Šiklová
    Nutrients, 2021-12-29;14(1):.
    Species: Human
    Sample Types: Serum
  20. Plasma Leptin Is Increased in Intensive Care Patients with COVID-19-An Investigation Performed in the PronMed-Cohort
    Authors: A Larsson, M Lipcsey, M Hultström, R Frithiof, M Eriksson
    Biomedicines, 2021-12-21;10(1):.
    Species: Human
    Sample Types: Plasma
  21. Adiponectin and Stnfr2 peripheral levels are associated with cardiovascular risk in patients with schizophrenia
    Authors: ICS Dias, SM de Campos-, ELM Vieira, APL Mota, PS Azevedo, VTDS Anício, FC Guimarães, LM Mantovani, BF Cruz, AL Teixeira, JV Salgado
    Journal of psychiatric research, 2021-11-09;0(0):.
    Species: Human
    Sample Types: Serum
  22. Gestational Weight Gain Influences the Adipokine-Oxidative Stress Association during Pregnancy
    Authors: JM Solis Pare, O Perichart, A Montoya Es, E Reyes Muño, S Espino Y S, V Ortega Cas, D Medina Bas, M Tolentino, M Sanchez Ma, S Nava Salaz, G Estrada Gu
    Obesity facts, 2021-09-14;0(0):1-9.
    Species: Human
    Sample Types: Serum
  23. Altered cardiometabolic profile in girls with central precocious puberty and adipokines: A propensity score matching analysis
    Authors: JN Zurita-Cru, MA Villasís-K, L Manuel-Apo, L Damasio-Sa, A Gutierrez-, G Wakida-Kus, M Padilla-Ro, C Maldonado-, E Garrido-Ma, AJ Rivera-Her, E Nishimura-
    Cytokine, 2021-07-30;0(0):155660.
    Species: Human
    Sample Types: Serum
  24. Fecal microbial transplantation and fiber supplementation in patients with severe obesity and metabolic syndrome: a randomized double-blind, placebo-controlled phase 2 trial
    Authors: V Mocanu, Z Zhang, EC Deehan, DH Kao, N Hotte, S Karmali, DW Birch, KK Samarasing, J Walter, KL Madsen
    Nature Medicine, 2021-07-05;27(7):1272-1279.
    Species: Human
    Sample Types: Serum
  25. Alternatives for the worse: Molecular insights into adverse effects of bisphenol a and substitutes during human adipocyte differentiation
    Authors: A Schaffert, L Krieg, J Weiner, R Schlichtin, E Ueberham, I Karkossa, M Bauer, K Landgraf, KM Junge, M Wabitsch, J Lehmann, BI Escher, AC Zenclussen, A Körner, M Blüher, JT Heiker, M von Bergen, K Schubert
    Environment international, 2021-06-27;156(0):106730.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. The dynamics of human bone marrow adipose tissue in response to feeding and fasting
    Authors: PK Fazeli, MA Bredella, OG Pachon-Peñ, W Zhao, X Zhang, AT Faje, M Resulaj, SP Polineni, TM Holmes, H Lee, EK O'Donnell, OA MacDougald, MC Horowitz, CJ Rosen, A Klibanski
    JCI Insight, 2021-06-22;0(0):.
    Species: Human
    Sample Types: Serum
  27. Predictors of Early-Recurrence Atrial Fibrillation after Catheter Ablation in Women and Men with Abnormal Body Weight
    Authors: J Budzianows, J Hiczkiewic, K ?ojewska, E Kawka, R Rutkowski, K Korybalska
    Journal of Clinical Medicine, 2021-06-18;10(12):.
    Species: Human
    Sample Types: Serum
  28. Reduction in Maternal Energy Intake during Lactation Decreased Maternal Body Weight and Concentrations of Leptin, Insulin and Adiponectin in Human Milk without Affecting Milk Production, Milk Macronutrient Composition or Infant Growth
    Authors: GE Leghi, MJ Netting, CT Lai, A Narayanan, M Dymock, A Rea, ME Wlodek, DT Geddes, BS Muhlhausle
    Nutrients, 2021-05-31;13(6):.
    Species: Human
    Sample Types: Breast Milk
  29. Impact of the Uncoupling Protein 1 on Cardiovascular Risk in Patients with Rheumatoid Arthritis
    Authors: LI Lyngfelt, MC Erlandsson, M Nadali, S Hedjazifar, R Pullerits, KM Andersson, P Brembeck, ST Silfverswä, U Smith, MI Bokarewa
    Cells, 2021-05-07;10(5):.
    Species: Human
    Sample Types: Serum
  30. The effect of Gestational Diabetes Mellitus on the fetal compartment
    Authors: TPB De Luccia, E Ono, R Menon, AU Borbely, R Mattar, L Richardson, ALM da Silva, RM Botelho, MLTLF da Rocha, S Daher
    Journal of reproductive immunology, 2021-03-24;145(0):103314.
    Species: Human
    Sample Types: Serum
  31. Body mass index and serum levels of soluble leptin receptor are sex-specifically related to alcohol binge drinking behavior
    Authors: P Bouna-Pyrr, C Muehle, J Kornhuber, C Weinland, B Lenz
    Psychoneuroendocrinology, 2021-02-25;127(0):105179.
    Species: Human
    Sample Types: Serum
  32. Reduced concentrations of the B cell cytokine Interleukin 38 are associated with cardiovascular disease risk in overweight subjects
    Authors: DM de Graaf, M Jaeger, ICL van den Mu, RT Horst, K Schraa, J Zwaag, M Kox, M Fujita, T Yamauchi, L Mercurio, S Madonna, JHW Rutten, J de Graaf, NP Riksen, FL van de Vee, MG Netea, LAB Joosten, CA Dinarello
    Eur J Immunol, 2020-11-19;0(0):.
    Species: Human
    Sample Types: Plasma
  33. First trimester secreted Frizzled-Related Protein 4 and other adipokine serum concentrations in women developing gestational diabetes mellitus
    Authors: JHN Schuitemak, RHJ Beernink, A Franx, TIFH Cremers, MPH Koster
    PLoS ONE, 2020-11-18;15(11):e0242423.
    Species: Human
    Sample Types: Serum
  34. BCG vaccination in humans inhibits systemic inflammation in a sex-dependent manner
    Authors: VACM Koeken, LCJ de Bree, VP Mourits, SJ Moorlag, J Walk, B Cirovic, RJ Arts, M Jaeger, H Dijkstra, H Lemmers, LA Joosten, CS Benn, R van Crevel, M Netea
    J. Clin. Invest., 2020-10-01;0(0):.
    Species: Human
    Sample Types: Plasma
  35. Maternal Anthropometric Factors and Circulating Adipokines as Predictors of Birth Weight and Length
    Authors: D Mazurek, M Bronkowska
    Int J Environ Res Public Health, 2020-07-03;17(13):.
    Species: Human
    Sample Types: Plasma
  36. Using a cultural dance program to increase sustainable physical activity for breast cancer survivors-A pilot study
    Authors: LWM Loo, K Nishibun, L Welsh, T Makolo, CD Chong, I Pagano, H Yu, EO Bantum
    Complement Ther Med, 2019-09-21;47(0):102197.
    Species: Human
    Sample Types: Plasma
  37. Whey Protein Supplementation Compared to Collagen Increases Blood Nesfatin Concentrations and Decreases Android Fat in Overweight Women: A Randomized Double-Blind Study
    Authors: BM Giglio, RM Schincagli, AS da Silva, ICS Fazani, PA Monteiro, JF Mota, JP Cunha, C Pichard, GD Pimentel
    Nutrients, 2019-09-02;11(9):.
    Species: Human
    Sample Types: Plasma
  38. Growth and differentiation factor 15 is a biomarker for low back pain-associated disability
    Authors: N Tarabeih, A Shalata, S Trofimov, A Kalinkovic, G Livshits
    Cytokine, 2019-02-15;117(0):8-14.
    Species: Human
    Sample Types: Plasma
  39. Impact to short-term high intensity intermittent training on different storages of body fat, leptin and soluble leptin receptor levels in physically active non-obese men: A pilot investigation
    Authors: RS Caldeira, VLG Panissa, DS Inoue, EZ Campos, PA Monteiro, BM Giglio, GD Pimentel, P Hofmann, FS Lira
    Clin Nutr ESPEN, 2018-09-07;28(0):186-192.
    Species: Human
    Sample Types: Serum
  40. Effects of the inspiratory muscle training and aerobic training on respiratory and functional parameters, inflammatory biomarkers, redox status and quality of life in hemodialysis patients: A randomized clinical trial
    Authors: PHS Figueiredo, MMO Lima, HS Costa, JB Martins, OD Flecha, PF Gonçalves, FL Alves, VGB Rodrigues, EHB Maciel, VA Mendonça, ACR Lacerda, ÉLM Vieira, AL Teixeira, F de Paula, CH Balthazar
    PLoS ONE, 2018-07-26;13(7):e0200727.
    Species: Human
    Sample Types: Plasma
  41. Impact of rural-urban environment on metabolic profile and response to a 5-day high-fat diet
    Authors: DL Tahapary, K de Ruiter, F Kurniawan, Y Djuardi, Y Wang, SME Nurdin, E Iskandar, D Minggu, E Yunir, B Guigas, T Supali, PCN Rensen, E Sartono, P Soewondo, DS Harbuwono, JWA Smit, M Yazdanbakh
    Sci Rep, 2018-05-25;8(1):8149.
    Species: Human
    Sample Types: Plasma
  42. The effects of intensified training on resting metabolic rate (RMR), body composition and performance in trained cyclists
    Authors: AL Woods, AJ Rice, LA Garvican-L, AM Wallett, B Lundy, MA Rogers, M Welvaert, S Halson, A McKune, KG Thompson
    PLoS ONE, 2018-02-14;13(2):e0191644.
    Species: Human
    Sample Types: Serum
  43. Gender differences of cannabis smoking on serum leptin levels: population-based study
    Authors: FP Moreira, CD Wiener, JF Oliveira, LDM Souza, RA da Silva, LV Portela, DR Lara, K Jansen, JP Oses
    Rev Bras Psiquiatr, 2018-02-01;0(0):0.
    Species: Human
    Sample Types: Serum
  44. High Expression of STAT3 in Subcutaneous Adipose Tissue Associates with Cardiovascular Risk in Women with Rheumatoid Arthritis
    Authors: M Nadali, R Pullerits, KME Andersson, ST Silfverswä, MC Erlandsson, MI Bokarewa
    Int J Mol Sci, 2017-11-13;18(11):.
    Species: Human
    Sample Types: Serum
  45. The adipokine leptin modulates adventitial pericyte functions by autocrine and paracrine signalling
    Authors: F Riu, SC Slater, EJ Garcia, I Rodriguez-, V Alvino, E Avolio, G Mangialard, A Cordaro, S Satchell, C Zebele, A Caporali, G Angelini, P Madeddu
    Sci Rep, 2017-07-14;7(1):5443.
    Species: Human
    Sample Types: Cell Culture Supernates
  46. Presence of IL-17 in synovial fluid identifies a potential inflammatory osteoarthritic phenotype
    Authors: SJ Snelling, S Bas, GJ Puskas, SG Dakin, D Suva, A Finckh, C Gabay, P Hoffmeyer, AJ Carr, A Lübbeke
    PLoS ONE, 2017-04-11;12(4):e0175109.
    Species: Human
    Sample Types: Serum
  47. Associations between Maternal Body Composition and Appetite Hormones and Macronutrients in Human Milk
    Authors: S Kuganantha, Z Gridneva, CT Lai, AR Hepworth, PJ Mark, F Kakulas, DT Geddes
    Nutrients, 2017-03-09;9(3):.
    Species: Human
    Sample Types: Milk
  48. Obesity-induced diet leads to weight gain, systemic metabolic alterations, adipose tissue inflammation, hepatic steatosis, and oxidative stress in gerbils (Meriones unguiculatus)
    Authors: LL Ventura, NC Fortes, HC Santiago, MV Caliari, MA Gomes, DR Oliveira
    PeerJ, 2017-03-02;5(0):e2967.
    Species: Gerbil
    Sample Types: Tissue Homogenates
  49. Effect of Human Milk Appetite Hormones, Macronutrients, and Infant Characteristics on Gastric Emptying and Breastfeeding Patterns of Term Fully Breastfed Infants
    Authors: Zoya Gridneva
    Nutrients, 2016-12-28;9(1):.
    Species: Human
    Sample Types: Breast Milk
  50. Secretory activity of subcutaneous abdominal adipose tissue in male patients with rheumatoid arthritis and osteoarthritis - association with clinical and laboratory data
    Authors: Ewa Kontny
    Reumatologia, 2016-11-28;54(5):227-235.
    Species: Human
    Sample Types: Tissue Extracts
  51. Association study of arcuate nucleus neuropeptide Y neuron receptor gene variation and serum NPY levels in clozapine treated patients with schizophrenia
    Authors: E Leinonen
    Eur. Psychiatry, 2016-11-10;40(0):13-19.
    Species: Human
    Sample Types: Serum
  52. Leptin Levels Are Higher in Whole Compared to Skim Human Milk, Supporting a Cellular Contribution
    Authors: Foteini Kakulas
    Nutrients, 2016-11-08;8(11):.
    Species: Human
    Sample Types: Milk
  53. A Multifactorial Weight Reduction Programme for Children with Overweight and Asthma: A Randomized Controlled Trial
    PLoS ONE, 2016-06-13;11(6):e0157158.
    Species: Human
    Sample Types: Serum
  54. Characterization of In Vitro Engineered Human Adipose Tissues: Relevant Adipokine Secretion and Impact of TNF-alpha.
    Authors: Aubin K, Safoine M, Proulx M, Audet-Casgrain M, Côté J, Tetu F, Roy A, Fradette J
    PLoS ONE, 2015-09-14;10(9):e0137612.
    Species: Human
    Sample Types: Cell Culture Supernates
  55. Human adipose tissue conditioned media from lean subjects is protective against H2O2 induced neurotoxicity in human SH-SY5Y neuronal cells.
    Authors: Wan Z, Mah D, Simtchouk S, Kluftinger A, Little J
    Int J Mol Sci, 2015-01-06;16(1):1221-31.
    Species: Human
    Sample Types: Cell Culture Supernates
  56. Expression of obesity markers and Persistent Organic Pollutants levels in adipose tissue of obese patients: reinforcing the obesogen hypothesis?
    Authors: Pereira-Fernandes A, Dirinck E, Dirtu A, Malarvannan G, Covaci A, Van Gaal L, Vanparys C, Jorens P, Blust R
    PLoS ONE, 2014-01-10;9(1):e84816.
    Species: Human
    Sample Types: Serum
  57. Endothelial cells from visceral adipose tissue disrupt adipocyte functions in a three-dimensional setting: partial rescue by angiopoietin-1.
    Authors: Pellegrinelli V, Rouault C, Veyrie N, Clement K, Lacasa D
    Diabetes, 2013-10-15;63(2):535-49.
    Species: Human
    Sample Types: Cell Culture Supernates
  58. Performance characteristics of combinations of host biomarkers to identify women with occult placental malaria: a case-control study from Malawi.
    Authors: Conroy AL, Liles WC, Molyneux ME
    PLoS ONE, 2011-12-12;6(12):e28540.
    Species: Human
    Sample Types: Plasma
  59. Adipokine resistin predicts anti-inflammatory effect of glucocorticoids in asthma.
    Authors: Leivo-Korpela S, Lehtimaki L, Vuolteenaho K, Nieminen R, Kankaanranta H, Saarelainen S, Moilanen E
    J Inflamm (Lond), 2011-05-26;8(0):12.
    Species: Human
    Sample Types: Plasma
  60. LIGHT (TNFSF14) inhibits adipose differentiation without affecting adipocyte metabolism.
    Authors: Tiller G, Laumen H, Fischer-Posovszky P, Finck A, Skurk T, Keuper M, Brinkmann U, Wabitsch M, Link D, Hauner H
    Int J Obes (Lond), 2010-06-15;35(2):208-16.
    Species: Human
    Sample Types: Cell Culture Supernates
  61. Regulation of adipokine production in human adipose tissue by propionic acid.
    Authors: Al-Lahham SH, Roelofsen H, Priebe M, Weening D, Dijkstra M, Hoek A, Rezaee F, Venema K, Vonk RJ
    Eur. J. Clin. Invest., 2010-03-25;40(5):401-7.
    Species: Human
    Sample Types: Cell Culture Supernates
  62. Homeostatic regulation of T effector to Treg ratios in an area of seasonal malaria transmission.
    Authors: Finney OC, Nwakanma D, Conway DJ, Walther M, Riley EM
    Eur. J. Immunol., 2009-05-01;39(5):1288-300.
    Species: Human
    Sample Types: Plasma
  63. Relationships between serum leptin and bone markers during stable weight, weight reduction and weight regain in male and female judoists.
    Authors: Prouteau S, Benhamou L, Courteix D
    Eur. J. Endocrinol., 2006-03-01;154(3):389-95.
    Species: Human
    Sample Types: Serum

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Reviews for Human Leptin DuoSet ELISA

Average Rating: 4.8 (Based on 5 Reviews)

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Human Leptin DuoSet ELISA
By Anonymous on 10/17/2023
Sample Tested: Serum
Human Leptin DuoSet ELISA
By Anonymous on 12/04/2019
Sample Tested: EDTA Plasma

diluted 1:50, followed the same procedures in the protocol.

Human Leptin DuoSet ELISA
By Carmen Martin-Ruiz on 08/14/2019
Sample Tested: Human serum

Sample diluted 1/40, very good replicates. Very reliable across plates. Used 384-well plate format

Human Leptin DuoSet ELISA DY398
Human Leptin DuoSet ELISA
By Balaji Mahender on 12/20/2017
Sample Tested: EDTA Plasma
Human Leptin DuoSet ELISA
By Priya Balasubramanian on 08/09/2017
Sample Tested: Serum

serum dilution used - 1:2.5

Human Leptin DuoSet ELISA DY398