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Human Laminin  alpha 3/Laminin-5 Antibody

Catalog #: MAB2144
4 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2144
MAB2144-SP
Laminin alpha 3/Laminin-5 in MCF 10A Human Cell Line.
2 Images
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Laminin  alpha 3/Laminin-5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Laminin alpha 3/Laminin‑5 in Western blots.
Source
Monoclonal Mouse IgG1 Clone # P3H9-2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
Wayner, E.A. (1993) J. Cell Biol. 121(5):1141; Gallatin, W.M. et al. (1989) Proc. Natl. Acad. Sci. USA 86(12):4654. This application was not tested by R&D Systems.
 
Immunohistochemistry
8-25 µg/mL
See below
Immunoprecipitation
Gallatin, W.M. et al. (1989) Proc. Natl. Acad. Sci. 86:4654.
 
Adhesion Blockade
Gonzales, M. et al. (1999) Mol. Biol. Cell 10:259.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Laminin a3/Laminin-5 antibody in MCF 10A Human Cell Line by Immunocytochemistry (ICC). View Larger

Laminin alpha 3/Laminin-5 in MCF 10A Human Cell Line. Laminin a3/Laminin-5 was detected in immersion fixed MCF 10A human breast epithelial cell line using 10 µg/mL Mouse Anti-Human Laminin a3/Laminin-5 Monoclonal Antibody (Catalog # MAB2144) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Laminin a3/Laminin-5 antibody in Human Skin by Immunohistochemistry (IHC-P). View Larger

Laminin alpha 3/Laminin‑5 in Human Skin. Laminin a3/Laminin-5 was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human Laminin a3/Laminin-5 Monoclonal Antibody (Catalog # MAB2144) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to cell in the epiderimis. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Laminin alpha 3/Laminin-5

Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. Through interactions with Integrins, Dystroglycan and other receptors, Laminins contribute to cell differentiation, cell shape and migration, and maintenance of tissue phenotypes and survival (1). Laminin-5, also known as Epiligrin, consists of an alpha 3, beta 3, and gamma 2 chain. It is abundant in transitional epithelium, stratified squamous epithelia, lung mucosa and other epithelial glands and plays an important role in the initiation and maintenance of epithelial cell anchorage to the underlying connective tissue (2, 3).

References
  1. Colognato, H. et al. (2000) Dev. Dyn. 218:213.
  2. Stahl, S. et al. (1997) J. Cell Sci. 110:55.
  3. Gonzales, M. et al. (1999) Mol. Biol. Cell 10:259.
Entrez Gene IDs
3909 (Human)
Alternate Names
a]3B;BM600;E170;LAMA3;Lama3B;LAMNA;LOCS; Epiligrin; LAMA3; Laminin alpha 3; Laminin5; Laminin-5

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Citations for Human Laminin  alpha 3/Laminin-5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Intraosseous transcutaneous amputation prostheses versus dental implants: A comparison between keratinocyte and gingival epithelial cell adhesion in vitro
    Authors: CJ Pendegrass, Centre for Biomedical Engineering, Institute of Orthopaedics and Musculoskeletal Science, University College London, Stanmore, UK, HT Lancashire, C Fontaine, G Chan, P Hosseini et al.
    European Cells and Materials
  2. A Novel Fluorescence-Based Screen of Gene Editing Molecules for Junctional Epidermolysis Bullosa
    Authors: J Zwicklhube, T Kocher, B Liemberger, S Hainzl, J Bischof, D Strunk, AM Raninger, I Gratz, V Wally, C Guttmann-G, JP Hofbauer, JW Bauer, U Koller
    International Journal of Molecular Sciences, 2023-03-08;24(6):.
  3. Functionalized Graphene Nanoparticles Induce Human Mesenchymal Stem Cells to Express Distinct Extracellular Matrix Proteins Mediating Osteogenesis
    Authors: SD Newby, T Masi, CD Griffin, WJ King, A Chipman, S Stephenson, DE Anderson, AS Biris, SE Bourdo, M Dhar
    Int J Nanomedicine, 2020-04-15;15(0):2501-2513.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC/IF
  4. Elastin fragments drive disease progression in a murine model of emphysema.
    Authors: Houghton AM, Quintero PA, Perkins DL, Kobayashi DK, Kelley DG, Marconcini LA, Mecham RP, Senior RM, Shapiro SD
    J. Clin. Invest., 2006-02-09;116(3):753-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Neutralization

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Reviews for Human Laminin  alpha 3/Laminin-5 Antibody

Average Rating: 4.7 (Based on 3 Reviews)

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Human Laminin  alpha 3/Laminin-5 Antibody
By Anonymous on 07/22/2021
Application: WB Sample Tested: Colonic cancer cells Species: Human
Human Laminin  alpha 3/Laminin-5 Antibody
By Anonymous on 07/08/2021
Application: IHC Sample Tested: Embryonic basement membrane Species: Human
Human Laminin  alpha 3/Laminin-5 Antibody
By Michelle Chen on 04/26/2017
Application: Immunocytochemistry/Immunofluorescence Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human
Immunocytochemistry/Immunofluorescence Human Laminin  alpha 3/Laminin-5 Antibody MAB2144